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Functional Cross-Talking between Differentially Expressed and Alternatively Spliced Genes in Human Liver Cancer Cells Treated with Berberine

Berberine has been identified with anti-proliferative effects on various cancer cells. Many researchers have been trying to elucidate the anti-cancer mechanisms of berberine based on differentially expressed genes. However, differentially alternative splicing genes induced by berberine might also co...

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Autores principales: Sheng, Zhen, Sun, Yi, Zhu, Ruixin, Jiao, Na, Tang, Kailin, Cao, Zhiwei, Ma, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659683/
https://www.ncbi.nlm.nih.gov/pubmed/26606055
http://dx.doi.org/10.1371/journal.pone.0143742
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author Sheng, Zhen
Sun, Yi
Zhu, Ruixin
Jiao, Na
Tang, Kailin
Cao, Zhiwei
Ma, Chao
author_facet Sheng, Zhen
Sun, Yi
Zhu, Ruixin
Jiao, Na
Tang, Kailin
Cao, Zhiwei
Ma, Chao
author_sort Sheng, Zhen
collection PubMed
description Berberine has been identified with anti-proliferative effects on various cancer cells. Many researchers have been trying to elucidate the anti-cancer mechanisms of berberine based on differentially expressed genes. However, differentially alternative splicing genes induced by berberine might also contribute to its pharmacological actions and have not been reported yet. Moreover, the potential functional cross-talking between the two sets of genes deserves further exploration. In this study, RNA-seq technology was used to detect the differentially expressed genes and differentially alternative spliced genes in BEL-7402 cancer cells induced by berberine. Functional enrichment analysis indicated that these genes were mainly enriched in the p53 and cell cycle signalling pathway. In addition, it was statistically proven that the two sets of genes were locally co-enriched along chromosomes, closely connected to each other based on protein-protein interaction and functionally similar on Gene Ontology tree. These results suggested that the two sets of genes regulated by berberine might be functionally cross-talked and jointly contribute to its cell cycle arresting effect. It has provided new clues for further researches on the pharmacological mechanisms of berberine as well as the other botanical drugs.
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spelling pubmed-46596832015-12-02 Functional Cross-Talking between Differentially Expressed and Alternatively Spliced Genes in Human Liver Cancer Cells Treated with Berberine Sheng, Zhen Sun, Yi Zhu, Ruixin Jiao, Na Tang, Kailin Cao, Zhiwei Ma, Chao PLoS One Research Article Berberine has been identified with anti-proliferative effects on various cancer cells. Many researchers have been trying to elucidate the anti-cancer mechanisms of berberine based on differentially expressed genes. However, differentially alternative splicing genes induced by berberine might also contribute to its pharmacological actions and have not been reported yet. Moreover, the potential functional cross-talking between the two sets of genes deserves further exploration. In this study, RNA-seq technology was used to detect the differentially expressed genes and differentially alternative spliced genes in BEL-7402 cancer cells induced by berberine. Functional enrichment analysis indicated that these genes were mainly enriched in the p53 and cell cycle signalling pathway. In addition, it was statistically proven that the two sets of genes were locally co-enriched along chromosomes, closely connected to each other based on protein-protein interaction and functionally similar on Gene Ontology tree. These results suggested that the two sets of genes regulated by berberine might be functionally cross-talked and jointly contribute to its cell cycle arresting effect. It has provided new clues for further researches on the pharmacological mechanisms of berberine as well as the other botanical drugs. Public Library of Science 2015-11-25 /pmc/articles/PMC4659683/ /pubmed/26606055 http://dx.doi.org/10.1371/journal.pone.0143742 Text en © 2015 Sheng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sheng, Zhen
Sun, Yi
Zhu, Ruixin
Jiao, Na
Tang, Kailin
Cao, Zhiwei
Ma, Chao
Functional Cross-Talking between Differentially Expressed and Alternatively Spliced Genes in Human Liver Cancer Cells Treated with Berberine
title Functional Cross-Talking between Differentially Expressed and Alternatively Spliced Genes in Human Liver Cancer Cells Treated with Berberine
title_full Functional Cross-Talking between Differentially Expressed and Alternatively Spliced Genes in Human Liver Cancer Cells Treated with Berberine
title_fullStr Functional Cross-Talking between Differentially Expressed and Alternatively Spliced Genes in Human Liver Cancer Cells Treated with Berberine
title_full_unstemmed Functional Cross-Talking between Differentially Expressed and Alternatively Spliced Genes in Human Liver Cancer Cells Treated with Berberine
title_short Functional Cross-Talking between Differentially Expressed and Alternatively Spliced Genes in Human Liver Cancer Cells Treated with Berberine
title_sort functional cross-talking between differentially expressed and alternatively spliced genes in human liver cancer cells treated with berberine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659683/
https://www.ncbi.nlm.nih.gov/pubmed/26606055
http://dx.doi.org/10.1371/journal.pone.0143742
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