Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay

This data article contains three figures and three videos related to the research article entitled “Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization” Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (...

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Autores principales: Zhang, Min, Song, Aihong, Lai, Siqiang, Qiu, Lisha, Huang, Yunlong, Chen, Qiang, Zhu, Bing, Xu, Dongsheng, Zheng, Jialin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659780/
https://www.ncbi.nlm.nih.gov/pubmed/26693502
http://dx.doi.org/10.1016/j.dib.2015.09.048
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author Zhang, Min
Song, Aihong
Lai, Siqiang
Qiu, Lisha
Huang, Yunlong
Chen, Qiang
Zhu, Bing
Xu, Dongsheng
Zheng, Jialin C.
author_facet Zhang, Min
Song, Aihong
Lai, Siqiang
Qiu, Lisha
Huang, Yunlong
Chen, Qiang
Zhu, Bing
Xu, Dongsheng
Zheng, Jialin C.
author_sort Zhang, Min
collection PubMed
description This data article contains three figures and three videos related to the research article entitled “Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization” Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (NPC) migration and polarization. The current article describes the neurosphere method used to culture NPCs. NPCs in neurospheres and monolayer were characterized using immunocytochemistry method with antibodies against two classic NPC markers: nestin and SOX2. The article also describes method to obtain sufficient protein lysates from NPCs in the stripe assay. When protein lysates were subjected to Rac1 affinity precipitation, Rac1-GTP was detected in the pull-down samples. In addition, the articles provides live cell imaging data to better understand CXCL12-mediated cellular migration and polarization.
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spelling pubmed-46597802015-12-21 Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay Zhang, Min Song, Aihong Lai, Siqiang Qiu, Lisha Huang, Yunlong Chen, Qiang Zhu, Bing Xu, Dongsheng Zheng, Jialin C. Data Brief Data Article This data article contains three figures and three videos related to the research article entitled “Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization” Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (NPC) migration and polarization. The current article describes the neurosphere method used to culture NPCs. NPCs in neurospheres and monolayer were characterized using immunocytochemistry method with antibodies against two classic NPC markers: nestin and SOX2. The article also describes method to obtain sufficient protein lysates from NPCs in the stripe assay. When protein lysates were subjected to Rac1 affinity precipitation, Rac1-GTP was detected in the pull-down samples. In addition, the articles provides live cell imaging data to better understand CXCL12-mediated cellular migration and polarization. Elsevier 2015-10-28 /pmc/articles/PMC4659780/ /pubmed/26693502 http://dx.doi.org/10.1016/j.dib.2015.09.048 Text en © 2015 Published by Elsevier Inc. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Data Article
Zhang, Min
Song, Aihong
Lai, Siqiang
Qiu, Lisha
Huang, Yunlong
Chen, Qiang
Zhu, Bing
Xu, Dongsheng
Zheng, Jialin C.
Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_full Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_fullStr Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_full_unstemmed Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_short Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_sort living cell imaging and rac1-gtp levels of cxcl12-treated migrating neural progenitor cells in stripe assay
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659780/
https://www.ncbi.nlm.nih.gov/pubmed/26693502
http://dx.doi.org/10.1016/j.dib.2015.09.048
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