Cargando…
Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction
This article describes additional data related to a research article entitled “Kinetics of Quadruplex to Duplex Conversion” (Mendoza et al. 2015 [1]). We followed the opening reaction of a series of intramolecular G-quadruplex structures by the addition of their corresponding complementary strand. F...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660241/ https://www.ncbi.nlm.nih.gov/pubmed/26693518 http://dx.doi.org/10.1016/j.dib.2015.10.039 |
_version_ | 1782402763606458368 |
---|---|
author | Mendoza, Oscar Elezgaray, Juan Mergny, Jean-Louis |
author_facet | Mendoza, Oscar Elezgaray, Juan Mergny, Jean-Louis |
author_sort | Mendoza, Oscar |
collection | PubMed |
description | This article describes additional data related to a research article entitled “Kinetics of Quadruplex to Duplex Conversion” (Mendoza et al. 2015 [1]). We followed the opening reaction of a series of intramolecular G-quadruplex structures by the addition of their corresponding complementary strand. Fluorolabeled complementary strands allowed to monitor the reaction in real-time. An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction. We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case. In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA). |
format | Online Article Text |
id | pubmed-4660241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-46602412015-12-21 Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction Mendoza, Oscar Elezgaray, Juan Mergny, Jean-Louis Data Brief Data Article This article describes additional data related to a research article entitled “Kinetics of Quadruplex to Duplex Conversion” (Mendoza et al. 2015 [1]). We followed the opening reaction of a series of intramolecular G-quadruplex structures by the addition of their corresponding complementary strand. Fluorolabeled complementary strands allowed to monitor the reaction in real-time. An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction. We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case. In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA). Elsevier 2015-11-05 /pmc/articles/PMC4660241/ /pubmed/26693518 http://dx.doi.org/10.1016/j.dib.2015.10.039 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Mendoza, Oscar Elezgaray, Juan Mergny, Jean-Louis Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction |
title | Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction |
title_full | Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction |
title_fullStr | Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction |
title_full_unstemmed | Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction |
title_short | Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction |
title_sort | spectroscopic data for the g-quadruplex dna to duplex dna reaction |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660241/ https://www.ncbi.nlm.nih.gov/pubmed/26693518 http://dx.doi.org/10.1016/j.dib.2015.10.039 |
work_keys_str_mv | AT mendozaoscar spectroscopicdataforthegquadruplexdnatoduplexdnareaction AT elezgarayjuan spectroscopicdataforthegquadruplexdnatoduplexdnareaction AT mergnyjeanlouis spectroscopicdataforthegquadruplexdnatoduplexdnareaction |