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IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES

The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF...

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Autores principales: CORRAL, Marcelo Andreetta, de PAULA, Fabiana Martins, GOTTARDI, Maiara, MEISEL, Dirce Mary Correia Lima, CASTILHO, Vera Lucia Pagliusi, GONÇALVES, Elenice Messias do Nascimento, CHIEFFI, Pedro Paulo, GRYSCHEK, Ronaldo Cesar Borges
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660453/
https://www.ncbi.nlm.nih.gov/pubmed/26603231
http://dx.doi.org/10.1590/S0036-46652015000500010
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author CORRAL, Marcelo Andreetta
de PAULA, Fabiana Martins
GOTTARDI, Maiara
MEISEL, Dirce Mary Correia Lima
CASTILHO, Vera Lucia Pagliusi
GONÇALVES, Elenice Messias do Nascimento
CHIEFFI, Pedro Paulo
GRYSCHEK, Ronaldo Cesar Borges
author_facet CORRAL, Marcelo Andreetta
de PAULA, Fabiana Martins
GOTTARDI, Maiara
MEISEL, Dirce Mary Correia Lima
CASTILHO, Vera Lucia Pagliusi
GONÇALVES, Elenice Messias do Nascimento
CHIEFFI, Pedro Paulo
GRYSCHEK, Ronaldo Cesar Borges
author_sort CORRAL, Marcelo Andreetta
collection PubMed
description The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.
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spelling pubmed-46604532015-12-10 IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES CORRAL, Marcelo Andreetta de PAULA, Fabiana Martins GOTTARDI, Maiara MEISEL, Dirce Mary Correia Lima CASTILHO, Vera Lucia Pagliusi GONÇALVES, Elenice Messias do Nascimento CHIEFFI, Pedro Paulo GRYSCHEK, Ronaldo Cesar Borges Rev Inst Med Trop Sao Paulo Brief Communication The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis. Instituto de Medicina Tropical 2015 /pmc/articles/PMC4660453/ /pubmed/26603231 http://dx.doi.org/10.1590/S0036-46652015000500010 Text en http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Brief Communication
CORRAL, Marcelo Andreetta
de PAULA, Fabiana Martins
GOTTARDI, Maiara
MEISEL, Dirce Mary Correia Lima
CASTILHO, Vera Lucia Pagliusi
GONÇALVES, Elenice Messias do Nascimento
CHIEFFI, Pedro Paulo
GRYSCHEK, Ronaldo Cesar Borges
IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES
title IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES
title_full IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES
title_fullStr IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES
title_full_unstemmed IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES
title_short IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES
title_sort immunodiagnosis of human strongyloidiasis: use of six different antigenic fractions from strongyloides venezuelensis parasitic females
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660453/
https://www.ncbi.nlm.nih.gov/pubmed/26603231
http://dx.doi.org/10.1590/S0036-46652015000500010
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