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IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES
The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto de Medicina Tropical
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660453/ https://www.ncbi.nlm.nih.gov/pubmed/26603231 http://dx.doi.org/10.1590/S0036-46652015000500010 |
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author | CORRAL, Marcelo Andreetta de PAULA, Fabiana Martins GOTTARDI, Maiara MEISEL, Dirce Mary Correia Lima CASTILHO, Vera Lucia Pagliusi GONÇALVES, Elenice Messias do Nascimento CHIEFFI, Pedro Paulo GRYSCHEK, Ronaldo Cesar Borges |
author_facet | CORRAL, Marcelo Andreetta de PAULA, Fabiana Martins GOTTARDI, Maiara MEISEL, Dirce Mary Correia Lima CASTILHO, Vera Lucia Pagliusi GONÇALVES, Elenice Messias do Nascimento CHIEFFI, Pedro Paulo GRYSCHEK, Ronaldo Cesar Borges |
author_sort | CORRAL, Marcelo Andreetta |
collection | PubMed |
description | The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis. |
format | Online Article Text |
id | pubmed-4660453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-46604532015-12-10 IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES CORRAL, Marcelo Andreetta de PAULA, Fabiana Martins GOTTARDI, Maiara MEISEL, Dirce Mary Correia Lima CASTILHO, Vera Lucia Pagliusi GONÇALVES, Elenice Messias do Nascimento CHIEFFI, Pedro Paulo GRYSCHEK, Ronaldo Cesar Borges Rev Inst Med Trop Sao Paulo Brief Communication The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis. Instituto de Medicina Tropical 2015 /pmc/articles/PMC4660453/ /pubmed/26603231 http://dx.doi.org/10.1590/S0036-46652015000500010 Text en http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Brief Communication CORRAL, Marcelo Andreetta de PAULA, Fabiana Martins GOTTARDI, Maiara MEISEL, Dirce Mary Correia Lima CASTILHO, Vera Lucia Pagliusi GONÇALVES, Elenice Messias do Nascimento CHIEFFI, Pedro Paulo GRYSCHEK, Ronaldo Cesar Borges IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES |
title | IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES |
title_full | IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES |
title_fullStr | IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES |
title_full_unstemmed | IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES |
title_short | IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES |
title_sort | immunodiagnosis of human strongyloidiasis: use of six different antigenic fractions from strongyloides venezuelensis parasitic females |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660453/ https://www.ncbi.nlm.nih.gov/pubmed/26603231 http://dx.doi.org/10.1590/S0036-46652015000500010 |
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