Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system

We present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor...

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Autores principales: Gang, Lin, Yao, Yu-chen, Liu, Ying-fu, Li, Yi-peng, Yang, Kai, Lu, Lei, Cheng, Yuan-chi, Chen, Xu-yi, Tu, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660754/
https://www.ncbi.nlm.nih.gov/pubmed/26692858
http://dx.doi.org/10.4103/1673-5374.167759
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author Gang, Lin
Yao, Yu-chen
Liu, Ying-fu
Li, Yi-peng
Yang, Kai
Lu, Lei
Cheng, Yuan-chi
Chen, Xu-yi
Tu, Yue
author_facet Gang, Lin
Yao, Yu-chen
Liu, Ying-fu
Li, Yi-peng
Yang, Kai
Lu, Lei
Cheng, Yuan-chi
Chen, Xu-yi
Tu, Yue
author_sort Gang, Lin
collection PubMed
description We present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor antagonist peptide (NEP1–40), which promotes axonal growth. Primary cultured oligodendrocytes suppressed axonal growth in the rat hippocampus, but NEP1–40 stimulated axonal growth in the co-culture system. Our results confirm the validity of the neuron-oligodendrocyte co-culture system as an assay for the evaluation of drugs for axon regeneration in the central nervous system.
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spelling pubmed-46607542015-12-11 Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system Gang, Lin Yao, Yu-chen Liu, Ying-fu Li, Yi-peng Yang, Kai Lu, Lei Cheng, Yuan-chi Chen, Xu-yi Tu, Yue Neural Regen Res Research Article We present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor antagonist peptide (NEP1–40), which promotes axonal growth. Primary cultured oligodendrocytes suppressed axonal growth in the rat hippocampus, but NEP1–40 stimulated axonal growth in the co-culture system. Our results confirm the validity of the neuron-oligodendrocyte co-culture system as an assay for the evaluation of drugs for axon regeneration in the central nervous system. Medknow Publications & Media Pvt Ltd 2015-10 /pmc/articles/PMC4660754/ /pubmed/26692858 http://dx.doi.org/10.4103/1673-5374.167759 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Gang, Lin
Yao, Yu-chen
Liu, Ying-fu
Li, Yi-peng
Yang, Kai
Lu, Lei
Cheng, Yuan-chi
Chen, Xu-yi
Tu, Yue
Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system
title Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system
title_full Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system
title_fullStr Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system
title_full_unstemmed Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system
title_short Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system
title_sort co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660754/
https://www.ncbi.nlm.nih.gov/pubmed/26692858
http://dx.doi.org/10.4103/1673-5374.167759
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