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Expanded endothelial progenitor cells mitigate lung injury in septic mice
Endothelial progenitor cells (EPCs) improve survival and reduce organ failure in cecal ligation and puncture-induced sepsis; however, expanded EPCs may represent an even better approach for vascular repair. To date, no study has compared the effects of non-expanded EPCs (EPC-NEXP) with those of expa...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660838/ https://www.ncbi.nlm.nih.gov/pubmed/26611795 http://dx.doi.org/10.1186/s13287-015-0226-7 |
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author | Güldner, Andreas Maron-Gutierrez, Tatiana Abreu, Soraia Carvalho Xisto, Debora Gonçalves Senegaglia, Alexandra Cristina Barcelos, Patty Rose da Silva Silva, Johnatas Dutra Brofman, Paulo Gama de Abreu, Marcelo Rocco, Patricia Rieken Macedo |
author_facet | Güldner, Andreas Maron-Gutierrez, Tatiana Abreu, Soraia Carvalho Xisto, Debora Gonçalves Senegaglia, Alexandra Cristina Barcelos, Patty Rose da Silva Silva, Johnatas Dutra Brofman, Paulo Gama de Abreu, Marcelo Rocco, Patricia Rieken Macedo |
author_sort | Güldner, Andreas |
collection | PubMed |
description | Endothelial progenitor cells (EPCs) improve survival and reduce organ failure in cecal ligation and puncture-induced sepsis; however, expanded EPCs may represent an even better approach for vascular repair. To date, no study has compared the effects of non-expanded EPCs (EPC-NEXP) with those of expanded EPCs (EPC-EXP) and mesenchymal stromal cells of human (MSC-HUMAN) and mouse (MSC-MICE) origin in experimental sepsis. One day after cecal ligation and puncture sepsis induction, BALB/c mice were randomized to receive saline, EPC-EXP, EPC-NEXP, MSC-HUMAN or MSC-MICE (1 × 10(5)) intravenously. EPC-EXP, EPC-NEXP, MSC-HUMAN, and MSC-MICE displayed differences in phenotypic characterization. On days 1 and 3, cecal ligation and puncture mice showed decreased survival rate, and increased elastance, diffuse alveolar damage, and levels of interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor-α, vascular endothelial growth factor, and platelet-derived growth factor in lung tissue. EPC-EXP and MSC-HUMAN had reduced elastance, diffuse alveolar damage, and platelet-derived growth factor compared to no-cell treatment. Tumor necrosis factor-α levels decreased in the EPC-EXP, MSC-HUMAN, and MSC-MICE groups. IL-1β levels decreased in the EPC-EXP group, while IL-10 decreased in the MSC-MICE. IL-6 levels decreased both in the EPC-EXP and MSC-MICE groups. Vascular endothelial growth factor levels were reduced regardless of therapy. In conclusion, EPC-EXP and MSC-HUMAN yielded better lung function and reduced histologic damage in septic mice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-015-0226-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4660838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46608382015-11-27 Expanded endothelial progenitor cells mitigate lung injury in septic mice Güldner, Andreas Maron-Gutierrez, Tatiana Abreu, Soraia Carvalho Xisto, Debora Gonçalves Senegaglia, Alexandra Cristina Barcelos, Patty Rose da Silva Silva, Johnatas Dutra Brofman, Paulo Gama de Abreu, Marcelo Rocco, Patricia Rieken Macedo Stem Cell Res Ther Short Report Endothelial progenitor cells (EPCs) improve survival and reduce organ failure in cecal ligation and puncture-induced sepsis; however, expanded EPCs may represent an even better approach for vascular repair. To date, no study has compared the effects of non-expanded EPCs (EPC-NEXP) with those of expanded EPCs (EPC-EXP) and mesenchymal stromal cells of human (MSC-HUMAN) and mouse (MSC-MICE) origin in experimental sepsis. One day after cecal ligation and puncture sepsis induction, BALB/c mice were randomized to receive saline, EPC-EXP, EPC-NEXP, MSC-HUMAN or MSC-MICE (1 × 10(5)) intravenously. EPC-EXP, EPC-NEXP, MSC-HUMAN, and MSC-MICE displayed differences in phenotypic characterization. On days 1 and 3, cecal ligation and puncture mice showed decreased survival rate, and increased elastance, diffuse alveolar damage, and levels of interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor-α, vascular endothelial growth factor, and platelet-derived growth factor in lung tissue. EPC-EXP and MSC-HUMAN had reduced elastance, diffuse alveolar damage, and platelet-derived growth factor compared to no-cell treatment. Tumor necrosis factor-α levels decreased in the EPC-EXP, MSC-HUMAN, and MSC-MICE groups. IL-1β levels decreased in the EPC-EXP group, while IL-10 decreased in the MSC-MICE. IL-6 levels decreased both in the EPC-EXP and MSC-MICE groups. Vascular endothelial growth factor levels were reduced regardless of therapy. In conclusion, EPC-EXP and MSC-HUMAN yielded better lung function and reduced histologic damage in septic mice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-015-0226-7) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-26 /pmc/articles/PMC4660838/ /pubmed/26611795 http://dx.doi.org/10.1186/s13287-015-0226-7 Text en © Güldner et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Güldner, Andreas Maron-Gutierrez, Tatiana Abreu, Soraia Carvalho Xisto, Debora Gonçalves Senegaglia, Alexandra Cristina Barcelos, Patty Rose da Silva Silva, Johnatas Dutra Brofman, Paulo Gama de Abreu, Marcelo Rocco, Patricia Rieken Macedo Expanded endothelial progenitor cells mitigate lung injury in septic mice |
title | Expanded endothelial progenitor cells mitigate lung injury in septic mice |
title_full | Expanded endothelial progenitor cells mitigate lung injury in septic mice |
title_fullStr | Expanded endothelial progenitor cells mitigate lung injury in septic mice |
title_full_unstemmed | Expanded endothelial progenitor cells mitigate lung injury in septic mice |
title_short | Expanded endothelial progenitor cells mitigate lung injury in septic mice |
title_sort | expanded endothelial progenitor cells mitigate lung injury in septic mice |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660838/ https://www.ncbi.nlm.nih.gov/pubmed/26611795 http://dx.doi.org/10.1186/s13287-015-0226-7 |
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