Formaldehyde scavengers function as novel antigen retrieval agents

Antigen retrieval agents improve the detection of formaldehyde-fixed proteins, but how they work is not well understood. We demonstrate that formaldehyde scavenging represents a key characteristic associated with effective antigen retrieval; under controlled temperature and pH conditions, scavenging...

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Detalles Bibliográficos
Autores principales: Vollert, Craig T., Moree, Wilna J., Gregory, Steven, Bark, Steven J., Eriksen, Jason L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661444/
https://www.ncbi.nlm.nih.gov/pubmed/26612041
http://dx.doi.org/10.1038/srep17322
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author Vollert, Craig T.
Moree, Wilna J.
Gregory, Steven
Bark, Steven J.
Eriksen, Jason L.
author_facet Vollert, Craig T.
Moree, Wilna J.
Gregory, Steven
Bark, Steven J.
Eriksen, Jason L.
author_sort Vollert, Craig T.
collection PubMed
description Antigen retrieval agents improve the detection of formaldehyde-fixed proteins, but how they work is not well understood. We demonstrate that formaldehyde scavenging represents a key characteristic associated with effective antigen retrieval; under controlled temperature and pH conditions, scavenging improves the typical antigen retrieval process through reversal of formaldehyde-protein adduct formation. This approach provides a rational framework for the identification and development of more effective antigen retrieval agents.
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spelling pubmed-46614442015-12-02 Formaldehyde scavengers function as novel antigen retrieval agents Vollert, Craig T. Moree, Wilna J. Gregory, Steven Bark, Steven J. Eriksen, Jason L. Sci Rep Article Antigen retrieval agents improve the detection of formaldehyde-fixed proteins, but how they work is not well understood. We demonstrate that formaldehyde scavenging represents a key characteristic associated with effective antigen retrieval; under controlled temperature and pH conditions, scavenging improves the typical antigen retrieval process through reversal of formaldehyde-protein adduct formation. This approach provides a rational framework for the identification and development of more effective antigen retrieval agents. Nature Publishing Group 2015-11-27 /pmc/articles/PMC4661444/ /pubmed/26612041 http://dx.doi.org/10.1038/srep17322 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Vollert, Craig T.
Moree, Wilna J.
Gregory, Steven
Bark, Steven J.
Eriksen, Jason L.
Formaldehyde scavengers function as novel antigen retrieval agents
title Formaldehyde scavengers function as novel antigen retrieval agents
title_full Formaldehyde scavengers function as novel antigen retrieval agents
title_fullStr Formaldehyde scavengers function as novel antigen retrieval agents
title_full_unstemmed Formaldehyde scavengers function as novel antigen retrieval agents
title_short Formaldehyde scavengers function as novel antigen retrieval agents
title_sort formaldehyde scavengers function as novel antigen retrieval agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661444/
https://www.ncbi.nlm.nih.gov/pubmed/26612041
http://dx.doi.org/10.1038/srep17322
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