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In Utero Exposure to Diethylhexyl Phthalate Affects Rat Brain Development: A Behavioral and Genomic Approach

Diethylhexyl phthalate (DEHP) is one of the most widely utilized phthalate plasticizers. Previous studies have demonstrated that gestational or postnatal DEHP exposure induced adverse effects on rat brain development and function. In this study, we investigated the effects of gestational DEHP exposu...

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Autores principales: Lin, Han, Yuan, Kaiming, Li, Linyan, Liu, Shiwen, Li, Senlin, Hu, Guoxin, Lian, Qing-Quan, Ge, Ren-Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661608/
https://www.ncbi.nlm.nih.gov/pubmed/26516888
http://dx.doi.org/10.3390/ijerph121113696
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author Lin, Han
Yuan, Kaiming
Li, Linyan
Liu, Shiwen
Li, Senlin
Hu, Guoxin
Lian, Qing-Quan
Ge, Ren-Shan
author_facet Lin, Han
Yuan, Kaiming
Li, Linyan
Liu, Shiwen
Li, Senlin
Hu, Guoxin
Lian, Qing-Quan
Ge, Ren-Shan
author_sort Lin, Han
collection PubMed
description Diethylhexyl phthalate (DEHP) is one of the most widely utilized phthalate plasticizers. Previous studies have demonstrated that gestational or postnatal DEHP exposure induced adverse effects on rat brain development and function. In this study, we investigated the effects of gestational DEHP exposure on gene expression profiling in neonatal rat brain and cognitive function change at adulthood. Adult Sprague Dawley dams were orally treated with 10 or 750 mg/kg DEHP from gestational day 12 to 21. Some male pups were euthanized at postnatal day 1 for gene expression profiling, and the rest males were retained for water maze testing on postnatal day (PND) 56. DEHP showed dose-dependent impairment of learning and spatial memory from PND 56 to 63. Genome-wide microarray analysis showed that 10 and 750 mg/kg DEHP altered the gene expression in the neonatal rat brain. Ccnd1 and Cdc2, two critical genes for neuron proliferation, were significantly down-regulated by DEHP. Interestingly, 750 mg/kg DEHP significantly increased Pmch level. Our study demonstrated the changed gene expression patterns after in utero DEHP exposure might partially contribute to the deficit of cognitive function at adulthood.
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spelling pubmed-46616082015-12-10 In Utero Exposure to Diethylhexyl Phthalate Affects Rat Brain Development: A Behavioral and Genomic Approach Lin, Han Yuan, Kaiming Li, Linyan Liu, Shiwen Li, Senlin Hu, Guoxin Lian, Qing-Quan Ge, Ren-Shan Int J Environ Res Public Health Article Diethylhexyl phthalate (DEHP) is one of the most widely utilized phthalate plasticizers. Previous studies have demonstrated that gestational or postnatal DEHP exposure induced adverse effects on rat brain development and function. In this study, we investigated the effects of gestational DEHP exposure on gene expression profiling in neonatal rat brain and cognitive function change at adulthood. Adult Sprague Dawley dams were orally treated with 10 or 750 mg/kg DEHP from gestational day 12 to 21. Some male pups were euthanized at postnatal day 1 for gene expression profiling, and the rest males were retained for water maze testing on postnatal day (PND) 56. DEHP showed dose-dependent impairment of learning and spatial memory from PND 56 to 63. Genome-wide microarray analysis showed that 10 and 750 mg/kg DEHP altered the gene expression in the neonatal rat brain. Ccnd1 and Cdc2, two critical genes for neuron proliferation, were significantly down-regulated by DEHP. Interestingly, 750 mg/kg DEHP significantly increased Pmch level. Our study demonstrated the changed gene expression patterns after in utero DEHP exposure might partially contribute to the deficit of cognitive function at adulthood. MDPI 2015-10-28 2015-11 /pmc/articles/PMC4661608/ /pubmed/26516888 http://dx.doi.org/10.3390/ijerph121113696 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Han
Yuan, Kaiming
Li, Linyan
Liu, Shiwen
Li, Senlin
Hu, Guoxin
Lian, Qing-Quan
Ge, Ren-Shan
In Utero Exposure to Diethylhexyl Phthalate Affects Rat Brain Development: A Behavioral and Genomic Approach
title In Utero Exposure to Diethylhexyl Phthalate Affects Rat Brain Development: A Behavioral and Genomic Approach
title_full In Utero Exposure to Diethylhexyl Phthalate Affects Rat Brain Development: A Behavioral and Genomic Approach
title_fullStr In Utero Exposure to Diethylhexyl Phthalate Affects Rat Brain Development: A Behavioral and Genomic Approach
title_full_unstemmed In Utero Exposure to Diethylhexyl Phthalate Affects Rat Brain Development: A Behavioral and Genomic Approach
title_short In Utero Exposure to Diethylhexyl Phthalate Affects Rat Brain Development: A Behavioral and Genomic Approach
title_sort in utero exposure to diethylhexyl phthalate affects rat brain development: a behavioral and genomic approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661608/
https://www.ncbi.nlm.nih.gov/pubmed/26516888
http://dx.doi.org/10.3390/ijerph121113696
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