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Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation

Soy isoflavones, genistein, daidzein and its metabolite equol, as well as β-carotene have been reported to be effective for maintaining bone health. However, it remains to be elucidated whether combining soy isoflavones with β-carotene is beneficial to bone formation. This study investigated the com...

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Autores principales: Nishide, Yoriko, Tousen, Yuko, Tadaishi, Miki, Inada, Masaki, Miyaura, Chisato, Kruger, Marlena C., Ishimi, Yoshiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661612/
https://www.ncbi.nlm.nih.gov/pubmed/26516892
http://dx.doi.org/10.3390/ijerph121113750
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author Nishide, Yoriko
Tousen, Yuko
Tadaishi, Miki
Inada, Masaki
Miyaura, Chisato
Kruger, Marlena C.
Ishimi, Yoshiko
author_facet Nishide, Yoriko
Tousen, Yuko
Tadaishi, Miki
Inada, Masaki
Miyaura, Chisato
Kruger, Marlena C.
Ishimi, Yoshiko
author_sort Nishide, Yoriko
collection PubMed
description Soy isoflavones, genistein, daidzein and its metabolite equol, as well as β-carotene have been reported to be effective for maintaining bone health. However, it remains to be elucidated whether combining soy isoflavones with β-carotene is beneficial to bone formation. This study investigated the combined effect of soy isoflavones and β-carotene on the differentiation of MC3T3-E1 preosteoblastic cells. Daidzein and genistein alone did not affect cell growth but increased alkaline phosphatase (ALP) activity. Beta-carotene alone inhibited cell growth and markedly enhanced ALP activity. Soy isoflavones combined with β-carotene resulted in higher ALP activity than treatment with isoflavones or β-carotene alone. We observed significant main effects of β-carotene on the enhanced expression of Runx2, ALP, and ostepontin mRNA, whereas there was a significant main effect of soy isoflavones on the expression of osterix mRNA. To investigate how β-carotene affected osteoblast differentiation, MC3T3-E1 cells were treated with retinoic acid receptor (RAR) pan-antagonist combined with β-carotene. Osteopontin and ALP mRNA expression levels, which were increased following treatment with β-carotene, were significantly suppressed by the RAR pan-antagonist. This suggests treatment with β-carotene enhanced early osteoblastic differentiation, at least in part via RAR signaling. These results indicate that a combination of isoflavones and β-carotene may be useful for maintaining a positive balance of bone turnover by inducing osteoblast differentiation.
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spelling pubmed-46616122015-12-10 Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation Nishide, Yoriko Tousen, Yuko Tadaishi, Miki Inada, Masaki Miyaura, Chisato Kruger, Marlena C. Ishimi, Yoshiko Int J Environ Res Public Health Article Soy isoflavones, genistein, daidzein and its metabolite equol, as well as β-carotene have been reported to be effective for maintaining bone health. However, it remains to be elucidated whether combining soy isoflavones with β-carotene is beneficial to bone formation. This study investigated the combined effect of soy isoflavones and β-carotene on the differentiation of MC3T3-E1 preosteoblastic cells. Daidzein and genistein alone did not affect cell growth but increased alkaline phosphatase (ALP) activity. Beta-carotene alone inhibited cell growth and markedly enhanced ALP activity. Soy isoflavones combined with β-carotene resulted in higher ALP activity than treatment with isoflavones or β-carotene alone. We observed significant main effects of β-carotene on the enhanced expression of Runx2, ALP, and ostepontin mRNA, whereas there was a significant main effect of soy isoflavones on the expression of osterix mRNA. To investigate how β-carotene affected osteoblast differentiation, MC3T3-E1 cells were treated with retinoic acid receptor (RAR) pan-antagonist combined with β-carotene. Osteopontin and ALP mRNA expression levels, which were increased following treatment with β-carotene, were significantly suppressed by the RAR pan-antagonist. This suggests treatment with β-carotene enhanced early osteoblastic differentiation, at least in part via RAR signaling. These results indicate that a combination of isoflavones and β-carotene may be useful for maintaining a positive balance of bone turnover by inducing osteoblast differentiation. MDPI 2015-10-28 2015-11 /pmc/articles/PMC4661612/ /pubmed/26516892 http://dx.doi.org/10.3390/ijerph121113750 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nishide, Yoriko
Tousen, Yuko
Tadaishi, Miki
Inada, Masaki
Miyaura, Chisato
Kruger, Marlena C.
Ishimi, Yoshiko
Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation
title Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation
title_full Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation
title_fullStr Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation
title_full_unstemmed Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation
title_short Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation
title_sort combined effects of soy isoflavones and β-carotene on osteoblast differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661612/
https://www.ncbi.nlm.nih.gov/pubmed/26516892
http://dx.doi.org/10.3390/ijerph121113750
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