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Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai

The Pacific abalone Haliotis discus hannai is used for commercial aquaculture in Korea. We examined the transcriptome of Pacific abalone Haliotis discus hannai siblings using NGS technology to identify genes associated with high growth rates. Pacific abalones grown for 200 days post-fertilization we...

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Autores principales: Choi, Mi-Jin, Kim, Gun-Do, Kim, Jong-Myoung, Lim, Han Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661900/
https://www.ncbi.nlm.nih.gov/pubmed/26593905
http://dx.doi.org/10.3390/ijms161126042
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author Choi, Mi-Jin
Kim, Gun-Do
Kim, Jong-Myoung
Lim, Han Kyu
author_facet Choi, Mi-Jin
Kim, Gun-Do
Kim, Jong-Myoung
Lim, Han Kyu
author_sort Choi, Mi-Jin
collection PubMed
description The Pacific abalone Haliotis discus hannai is used for commercial aquaculture in Korea. We examined the transcriptome of Pacific abalone Haliotis discus hannai siblings using NGS technology to identify genes associated with high growth rates. Pacific abalones grown for 200 days post-fertilization were divided into small-, medium-, and large-size groups with mean weights of 0.26 ± 0.09 g, 1.43 ± 0.405 g, and 5.24 ± 1.09 g, respectively. RNA isolated from the soft tissues of each group was subjected to RNA sequencing. Approximately 1%–3% of the transcripts were differentially expressed in abalones, depending on the growth rate. RT-PCR was carried out on thirty four genes selected to confirm the relative differences in expression detected by RNA sequencing. Six differentially-expressed genes were identified as associated with faster growth of the Pacific abalone. These include five up-regulated genes (including one specific to females) encoding transcripts homologous to incilarin A, perlucin, transforming growth factor-beta-induced protein immunoglobulin-heavy chain 3 (ig-h3), vitelline envelope zona pellucida domain 4, and defensin, and one down-regulated gene encoding tomoregulin in large abalones. Most of the transcripts were expressed predominantly in the hepatopancreas. The genes identified in this study will lead to development of markers for identification of high-growth-rate abalones and female abalones.
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spelling pubmed-46619002015-12-10 Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai Choi, Mi-Jin Kim, Gun-Do Kim, Jong-Myoung Lim, Han Kyu Int J Mol Sci Article The Pacific abalone Haliotis discus hannai is used for commercial aquaculture in Korea. We examined the transcriptome of Pacific abalone Haliotis discus hannai siblings using NGS technology to identify genes associated with high growth rates. Pacific abalones grown for 200 days post-fertilization were divided into small-, medium-, and large-size groups with mean weights of 0.26 ± 0.09 g, 1.43 ± 0.405 g, and 5.24 ± 1.09 g, respectively. RNA isolated from the soft tissues of each group was subjected to RNA sequencing. Approximately 1%–3% of the transcripts were differentially expressed in abalones, depending on the growth rate. RT-PCR was carried out on thirty four genes selected to confirm the relative differences in expression detected by RNA sequencing. Six differentially-expressed genes were identified as associated with faster growth of the Pacific abalone. These include five up-regulated genes (including one specific to females) encoding transcripts homologous to incilarin A, perlucin, transforming growth factor-beta-induced protein immunoglobulin-heavy chain 3 (ig-h3), vitelline envelope zona pellucida domain 4, and defensin, and one down-regulated gene encoding tomoregulin in large abalones. Most of the transcripts were expressed predominantly in the hepatopancreas. The genes identified in this study will lead to development of markers for identification of high-growth-rate abalones and female abalones. MDPI 2015-11-18 /pmc/articles/PMC4661900/ /pubmed/26593905 http://dx.doi.org/10.3390/ijms161126042 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Choi, Mi-Jin
Kim, Gun-Do
Kim, Jong-Myoung
Lim, Han Kyu
Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai
title Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai
title_full Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai
title_fullStr Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai
title_full_unstemmed Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai
title_short Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai
title_sort differentially-expressed genes associated with faster growth of the pacific abalone, haliotis discus hannai
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661900/
https://www.ncbi.nlm.nih.gov/pubmed/26593905
http://dx.doi.org/10.3390/ijms161126042
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