The kin17 Protein in Murine Melanoma Cells

kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line...

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Autores principales: Ramos, Anelise C., Gaspar, Vanessa P., Kelmer, Sabrina M. G., Sellani, Tarciso A., Batista, Ana G. U., De Lima Neto, Quirino A., Rodrigues, Elaine G., Fernandez, Maria A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661930/
https://www.ncbi.nlm.nih.gov/pubmed/26610484
http://dx.doi.org/10.3390/ijms161126072
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author Ramos, Anelise C.
Gaspar, Vanessa P.
Kelmer, Sabrina M. G.
Sellani, Tarciso A.
Batista, Ana G. U.
De Lima Neto, Quirino A.
Rodrigues, Elaine G.
Fernandez, Maria A.
author_facet Ramos, Anelise C.
Gaspar, Vanessa P.
Kelmer, Sabrina M. G.
Sellani, Tarciso A.
Batista, Ana G. U.
De Lima Neto, Quirino A.
Rodrigues, Elaine G.
Fernandez, Maria A.
author_sort Ramos, Anelise C.
collection PubMed
description kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10CR. Nuclear and chromatin-associated protein fractions were analyzed, and kin17 was detected in all fractions, with an elevated concentration observed in the chromatin-associated fraction of the clone with low metastatic potential, suggesting that the kin17 expression level could be a marker of melanoma.
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spelling pubmed-46619302015-12-10 The kin17 Protein in Murine Melanoma Cells Ramos, Anelise C. Gaspar, Vanessa P. Kelmer, Sabrina M. G. Sellani, Tarciso A. Batista, Ana G. U. De Lima Neto, Quirino A. Rodrigues, Elaine G. Fernandez, Maria A. Int J Mol Sci Article kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10CR. Nuclear and chromatin-associated protein fractions were analyzed, and kin17 was detected in all fractions, with an elevated concentration observed in the chromatin-associated fraction of the clone with low metastatic potential, suggesting that the kin17 expression level could be a marker of melanoma. MDPI 2015-11-24 /pmc/articles/PMC4661930/ /pubmed/26610484 http://dx.doi.org/10.3390/ijms161126072 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramos, Anelise C.
Gaspar, Vanessa P.
Kelmer, Sabrina M. G.
Sellani, Tarciso A.
Batista, Ana G. U.
De Lima Neto, Quirino A.
Rodrigues, Elaine G.
Fernandez, Maria A.
The kin17 Protein in Murine Melanoma Cells
title The kin17 Protein in Murine Melanoma Cells
title_full The kin17 Protein in Murine Melanoma Cells
title_fullStr The kin17 Protein in Murine Melanoma Cells
title_full_unstemmed The kin17 Protein in Murine Melanoma Cells
title_short The kin17 Protein in Murine Melanoma Cells
title_sort kin17 protein in murine melanoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661930/
https://www.ncbi.nlm.nih.gov/pubmed/26610484
http://dx.doi.org/10.3390/ijms161126072
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