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MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e

Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone mor...

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Autores principales: Liu, Zhicheng, Zhou, Yuru, Yuan, Yue, Nie, Fang, Peng, Rui, Li, Qianyin, Lyu, Zhongshi, Mao, Zhaomin, Huang, Liyuan, Zhou, Li, Li, Yiman, Hao, Jing, Ni, Dongsheng, Jin, Qianni, Long, Yaoshui, Ju, Pan, Yu, Wen, Liu, Jianing, Hu, Yanxia, Zhou, Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661932/
https://www.ncbi.nlm.nih.gov/pubmed/26610487
http://dx.doi.org/10.3390/ijms161126075
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author Liu, Zhicheng
Zhou, Yuru
Yuan, Yue
Nie, Fang
Peng, Rui
Li, Qianyin
Lyu, Zhongshi
Mao, Zhaomin
Huang, Liyuan
Zhou, Li
Li, Yiman
Hao, Jing
Ni, Dongsheng
Jin, Qianni
Long, Yaoshui
Ju, Pan
Yu, Wen
Liu, Jianing
Hu, Yanxia
Zhou, Qin
author_facet Liu, Zhicheng
Zhou, Yuru
Yuan, Yue
Nie, Fang
Peng, Rui
Li, Qianyin
Lyu, Zhongshi
Mao, Zhaomin
Huang, Liyuan
Zhou, Li
Li, Yiman
Hao, Jing
Ni, Dongsheng
Jin, Qianni
Long, Yaoshui
Ju, Pan
Yu, Wen
Liu, Jianing
Hu, Yanxia
Zhou, Qin
author_sort Liu, Zhicheng
collection PubMed
description Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3′UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7.
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spelling pubmed-46619322015-12-10 MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e Liu, Zhicheng Zhou, Yuru Yuan, Yue Nie, Fang Peng, Rui Li, Qianyin Lyu, Zhongshi Mao, Zhaomin Huang, Liyuan Zhou, Li Li, Yiman Hao, Jing Ni, Dongsheng Jin, Qianni Long, Yaoshui Ju, Pan Yu, Wen Liu, Jianing Hu, Yanxia Zhou, Qin Int J Mol Sci Article Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3′UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7. MDPI 2015-11-24 /pmc/articles/PMC4661932/ /pubmed/26610487 http://dx.doi.org/10.3390/ijms161126075 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhicheng
Zhou, Yuru
Yuan, Yue
Nie, Fang
Peng, Rui
Li, Qianyin
Lyu, Zhongshi
Mao, Zhaomin
Huang, Liyuan
Zhou, Li
Li, Yiman
Hao, Jing
Ni, Dongsheng
Jin, Qianni
Long, Yaoshui
Ju, Pan
Yu, Wen
Liu, Jianing
Hu, Yanxia
Zhou, Qin
MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e
title MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e
title_full MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e
title_fullStr MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e
title_full_unstemmed MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e
title_short MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e
title_sort mir542-3p regulates the epithelial-mesenchymal transition by directly targeting bmp7 in nrk52e
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661932/
https://www.ncbi.nlm.nih.gov/pubmed/26610487
http://dx.doi.org/10.3390/ijms161126075
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