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In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications
We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and CD NPs are conjugated with cationic FeAC NPs where CD NPs play a role in bio-imaging and Fe...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662025/ https://www.ncbi.nlm.nih.gov/pubmed/26612177 http://dx.doi.org/10.1186/s12951-015-0151-z |
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author | Kang, Kyoung Suk Lee, Hyun Uk Kim, Moon Il Park, So Young Chang, Sung-Jin Park, Ji-Ho Huh, Yun Suk Lee, Jouhahn Yang, Mino Lee, Young-Chul Park, Hyun Gyu |
author_facet | Kang, Kyoung Suk Lee, Hyun Uk Kim, Moon Il Park, So Young Chang, Sung-Jin Park, Ji-Ho Huh, Yun Suk Lee, Jouhahn Yang, Mino Lee, Young-Chul Park, Hyun Gyu |
author_sort | Kang, Kyoung Suk |
collection | PubMed |
description | We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and CD NPs are conjugated with cationic FeAC NPs where CD NPs play a role in bio-imaging and FeAC NPs act as a substrate for CD conjugation and help to uptake of NPs into cancer cells due to positively charged surface of FeAC NPs in physiological media. As increase of CD-FeAC NPs loading in HeLa cell in vitro, it showed slight cytotoxicity at 1000 μg/mL but no cytotoxicity for normal cells up to concentration of 1000 μg/mL confirmed by two 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, with further observations by 4′,6-diamidino-2-phenylindole (DAPI) stained confocal microscopy images, possessing that CD-FeAC NPs can be used as potential drug delivery platforms in cancer cells with simultaneous imaging. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12951-015-0151-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4662025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46620252015-11-28 In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications Kang, Kyoung Suk Lee, Hyun Uk Kim, Moon Il Park, So Young Chang, Sung-Jin Park, Ji-Ho Huh, Yun Suk Lee, Jouhahn Yang, Mino Lee, Young-Chul Park, Hyun Gyu J Nanobiotechnology Research We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and CD NPs are conjugated with cationic FeAC NPs where CD NPs play a role in bio-imaging and FeAC NPs act as a substrate for CD conjugation and help to uptake of NPs into cancer cells due to positively charged surface of FeAC NPs in physiological media. As increase of CD-FeAC NPs loading in HeLa cell in vitro, it showed slight cytotoxicity at 1000 μg/mL but no cytotoxicity for normal cells up to concentration of 1000 μg/mL confirmed by two 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, with further observations by 4′,6-diamidino-2-phenylindole (DAPI) stained confocal microscopy images, possessing that CD-FeAC NPs can be used as potential drug delivery platforms in cancer cells with simultaneous imaging. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12951-015-0151-z) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-26 /pmc/articles/PMC4662025/ /pubmed/26612177 http://dx.doi.org/10.1186/s12951-015-0151-z Text en © Kang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kang, Kyoung Suk Lee, Hyun Uk Kim, Moon Il Park, So Young Chang, Sung-Jin Park, Ji-Ho Huh, Yun Suk Lee, Jouhahn Yang, Mino Lee, Young-Chul Park, Hyun Gyu In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications |
title | In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications |
title_full | In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications |
title_fullStr | In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications |
title_full_unstemmed | In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications |
title_short | In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications |
title_sort | in-vitro cytotoxicity assessment of carbon-nanodot-conjugated fe-aminoclay (cd-feac) and its bio-imaging applications |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662025/ https://www.ncbi.nlm.nih.gov/pubmed/26612177 http://dx.doi.org/10.1186/s12951-015-0151-z |
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