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Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry

A rapid and simple analytical method for L-carnitine was developed for infant and toddler formulas by liquid chromatography tandem mass spectrometry (LC-MS/MS). A 0.3 g of infant formula and toddler formula sample was mixed in a 50 mL conical tube with 9 mL water and 1 mL 0.1 M hydrochloric acid (HC...

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Autores principales: Ahn, Jang-Hyuk, Kwak, Byung-Man, Park, Jung-Min, Kim, Na-Kyeoung, Kim, Jin-Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Food Science of Animal Resources 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662188/
https://www.ncbi.nlm.nih.gov/pubmed/26761670
http://dx.doi.org/10.5851/kosfa.2014.34.6.749
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author Ahn, Jang-Hyuk
Kwak, Byung-Man
Park, Jung-Min
Kim, Na-Kyeoung
Kim, Jin-Man
author_facet Ahn, Jang-Hyuk
Kwak, Byung-Man
Park, Jung-Min
Kim, Na-Kyeoung
Kim, Jin-Man
author_sort Ahn, Jang-Hyuk
collection PubMed
description A rapid and simple analytical method for L-carnitine was developed for infant and toddler formulas by liquid chromatography tandem mass spectrometry (LC-MS/MS). A 0.3 g of infant formula and toddler formula sample was mixed in a 50 mL conical tube with 9 mL water and 1 mL 0.1 M hydrochloric acid (HCl) to chemical extraction. Then, chloroform was used for removing a lipid fraction. After centrifuged, L-carnitine was separated and quantified using LC-MS/MS with electrospray ionization (ESI) mode. The precursor ion for L-carnitine was m/z 162, and product ions were m/z 103 (quantitative) and m/z 85 (qualitative), respectively. The results for spiked recovery test were in the range of 93.18-95.64% and the result for certified reference material (SRM 1849a) was within the range of the certificated values. This method could be implemented in many laboratories that require time and labor saving.
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spelling pubmed-46621882016-01-04 Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry Ahn, Jang-Hyuk Kwak, Byung-Man Park, Jung-Min Kim, Na-Kyeoung Kim, Jin-Man Korean J Food Sci Anim Resour Article A rapid and simple analytical method for L-carnitine was developed for infant and toddler formulas by liquid chromatography tandem mass spectrometry (LC-MS/MS). A 0.3 g of infant formula and toddler formula sample was mixed in a 50 mL conical tube with 9 mL water and 1 mL 0.1 M hydrochloric acid (HCl) to chemical extraction. Then, chloroform was used for removing a lipid fraction. After centrifuged, L-carnitine was separated and quantified using LC-MS/MS with electrospray ionization (ESI) mode. The precursor ion for L-carnitine was m/z 162, and product ions were m/z 103 (quantitative) and m/z 85 (qualitative), respectively. The results for spiked recovery test were in the range of 93.18-95.64% and the result for certified reference material (SRM 1849a) was within the range of the certificated values. This method could be implemented in many laboratories that require time and labor saving. Korean Society for Food Science of Animal Resources 2014 2014-12-31 /pmc/articles/PMC4662188/ /pubmed/26761670 http://dx.doi.org/10.5851/kosfa.2014.34.6.749 Text en Copyright © 2014, Korean Society for Food Science of Animal Resources http://creativecommons.org/licences/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Ahn, Jang-Hyuk
Kwak, Byung-Man
Park, Jung-Min
Kim, Na-Kyeoung
Kim, Jin-Man
Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry
title Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry
title_full Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry
title_fullStr Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry
title_full_unstemmed Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry
title_short Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry
title_sort rapid determination of l-carnitine in infant and toddler formulas by liquid chromatography tandem mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662188/
https://www.ncbi.nlm.nih.gov/pubmed/26761670
http://dx.doi.org/10.5851/kosfa.2014.34.6.749
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