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Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates

This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatme...

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Autores principales: Kim, Dongwook, Kim, Hee-Jin, Chae, Hyun-Seok, Park, Nam-Gun, Kim, Young-Boong, Jang, Aera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Food Science of Animal Resources 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662201/
https://www.ncbi.nlm.nih.gov/pubmed/26761683
http://dx.doi.org/10.5851/kosfa.2014.34.6.844
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author Kim, Dongwook
Kim, Hee-Jin
Chae, Hyun-Seok
Park, Nam-Gun
Kim, Young-Boong
Jang, Aera
author_facet Kim, Dongwook
Kim, Hee-Jin
Chae, Hyun-Seok
Park, Nam-Gun
Kim, Young-Boong
Jang, Aera
author_sort Kim, Dongwook
collection PubMed
description This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatment with LMP from JHLB was evaluated. Determination of the antioxidant activity of various concentrations of LMP from JHLB were carried out by assessing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethybenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). The DPPH radical scavenging activity of LMP from JHLB (20 mg/mL) was 92.21% and ABTS radical scavenging activity (15 mg/mL) was 99.50%. FRAP activity (30 mg/mL) was 364.72 μM/TE and ORAC activity (1 mg/mL) was 101.85 μM/TE. The anti-wrinkle potential was assessed by evaluating the elastase- and collagenase inhibition potential of these LMP. We found that 200 mg/mL of LMP from JHLB inhibited elastase activity by 41.32%, and 100 mg/mL of LMP from JHLB inhibited collagenase activity by 91.32%. The cell viability of untreated HS68 human dermal fibroblasts was 45% when exposed to a UVB radiation dose of 100 mJ/cm(2). After 24 h of incubation with 500 μg/mL LMP from JHLB, the cell viability increased to 60%. These results indicate that LMP from JHLB has potential utility as an anti-oxidant and anti-wrinkle agent in the food and cosmetic industry. Additional in vivo tests should be carried out to further characterize these potential benefits.
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spelling pubmed-46622012016-01-04 Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates Kim, Dongwook Kim, Hee-Jin Chae, Hyun-Seok Park, Nam-Gun Kim, Young-Boong Jang, Aera Korean J Food Sci Anim Resour Article This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatment with LMP from JHLB was evaluated. Determination of the antioxidant activity of various concentrations of LMP from JHLB were carried out by assessing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethybenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). The DPPH radical scavenging activity of LMP from JHLB (20 mg/mL) was 92.21% and ABTS radical scavenging activity (15 mg/mL) was 99.50%. FRAP activity (30 mg/mL) was 364.72 μM/TE and ORAC activity (1 mg/mL) was 101.85 μM/TE. The anti-wrinkle potential was assessed by evaluating the elastase- and collagenase inhibition potential of these LMP. We found that 200 mg/mL of LMP from JHLB inhibited elastase activity by 41.32%, and 100 mg/mL of LMP from JHLB inhibited collagenase activity by 91.32%. The cell viability of untreated HS68 human dermal fibroblasts was 45% when exposed to a UVB radiation dose of 100 mJ/cm(2). After 24 h of incubation with 500 μg/mL LMP from JHLB, the cell viability increased to 60%. These results indicate that LMP from JHLB has potential utility as an anti-oxidant and anti-wrinkle agent in the food and cosmetic industry. Additional in vivo tests should be carried out to further characterize these potential benefits. Korean Society for Food Science of Animal Resources 2014 2014-12-31 /pmc/articles/PMC4662201/ /pubmed/26761683 http://dx.doi.org/10.5851/kosfa.2014.34.6.844 Text en Copyright © 2014, Korean Society for Food Science of Animal Resources http://creativecommons.org/licences/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kim, Dongwook
Kim, Hee-Jin
Chae, Hyun-Seok
Park, Nam-Gun
Kim, Young-Boong
Jang, Aera
Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates
title Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates
title_full Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates
title_fullStr Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates
title_full_unstemmed Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates
title_short Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates
title_sort anti-oxidation and anti-wrinkling effects of jeju horse leg bone hydrolysates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662201/
https://www.ncbi.nlm.nih.gov/pubmed/26761683
http://dx.doi.org/10.5851/kosfa.2014.34.6.844
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