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Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice

BACKGROUND AND OBJECTIVES: Haemorrhagic septicaemia (HS), caused by Pasteurella multocida, is the most important bacterial disease of cattle and buffaloes in India. Oil adjuvant vaccine (OAV) is the most potent vaccine available for the control of HS. The study aims to evaluate the effect of alum co...

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Autores principales: Kumar, Sujeet, Chaturvedi, Vinod Kumar, Kumar, Bablu, Kumar, Pankaj, Somarajan, Sudha Rani, Mishra, Anil Kumar, Sharma, Bhaskar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662783/
https://www.ncbi.nlm.nih.gov/pubmed/26622968
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author Kumar, Sujeet
Chaturvedi, Vinod Kumar
Kumar, Bablu
Kumar, Pankaj
Somarajan, Sudha Rani
Mishra, Anil Kumar
Sharma, Bhaskar
author_facet Kumar, Sujeet
Chaturvedi, Vinod Kumar
Kumar, Bablu
Kumar, Pankaj
Somarajan, Sudha Rani
Mishra, Anil Kumar
Sharma, Bhaskar
author_sort Kumar, Sujeet
collection PubMed
description BACKGROUND AND OBJECTIVES: Haemorrhagic septicaemia (HS), caused by Pasteurella multocida, is the most important bacterial disease of cattle and buffaloes in India. Oil adjuvant vaccine (OAV) is the most potent vaccine available for the control of HS. The study aims to evaluate the effect of alum co-adjuvantation of OAV on emulsion stability and immune response. MATERIALS AND METHODS: Two different oil adjuvant vaccines viz., standard oil adjuvant vaccine (OAV) and alum precipitated oil adjuvant vaccine (A–OAV) were prepared with Pasteurella multocida antigen. Emulsion stability was tested by centrifugation, storage at 37 °C for 3 months and microscopy. Immune responses were evaluated by ELISA antibody titer, CD4, CD8 T cell populations and survival post challenge by P. multocida in mice. RESULTS: The separation of aqueous and oil phase of emulsion by centrifugation and storage test were 0 and 6.76% in A-OAV as compared to 11.00 and 26.39% in OAV, respectively. The mean droplet size was significantly smaller (p<0.01) in A–OAV as compared to OAV. The A–OAV recorded higher ELISA antibody titer (p<0.05) up to 21st days post vaccination, and higher CD4 (p>0.05) and CD8 T cell (p<0.05) populations compared to OAV. The A–OAV group conferred 100% protection after challenge with both 100 LD(50) and 1000 LD(50) as compared to 100 and 60% respective protection by OAV group. CONCLUSION: The results indicates that A–OAV had better emulsion stability, produces higher level of CD4, CD8 T cells and antibody titer with better protection compared to oil adjuvant vaccine.
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spelling pubmed-46627832015-11-30 Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice Kumar, Sujeet Chaturvedi, Vinod Kumar Kumar, Bablu Kumar, Pankaj Somarajan, Sudha Rani Mishra, Anil Kumar Sharma, Bhaskar Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Haemorrhagic septicaemia (HS), caused by Pasteurella multocida, is the most important bacterial disease of cattle and buffaloes in India. Oil adjuvant vaccine (OAV) is the most potent vaccine available for the control of HS. The study aims to evaluate the effect of alum co-adjuvantation of OAV on emulsion stability and immune response. MATERIALS AND METHODS: Two different oil adjuvant vaccines viz., standard oil adjuvant vaccine (OAV) and alum precipitated oil adjuvant vaccine (A–OAV) were prepared with Pasteurella multocida antigen. Emulsion stability was tested by centrifugation, storage at 37 °C for 3 months and microscopy. Immune responses were evaluated by ELISA antibody titer, CD4, CD8 T cell populations and survival post challenge by P. multocida in mice. RESULTS: The separation of aqueous and oil phase of emulsion by centrifugation and storage test were 0 and 6.76% in A-OAV as compared to 11.00 and 26.39% in OAV, respectively. The mean droplet size was significantly smaller (p<0.01) in A–OAV as compared to OAV. The A–OAV recorded higher ELISA antibody titer (p<0.05) up to 21st days post vaccination, and higher CD4 (p>0.05) and CD8 T cell (p<0.05) populations compared to OAV. The A–OAV group conferred 100% protection after challenge with both 100 LD(50) and 1000 LD(50) as compared to 100 and 60% respective protection by OAV group. CONCLUSION: The results indicates that A–OAV had better emulsion stability, produces higher level of CD4, CD8 T cells and antibody titer with better protection compared to oil adjuvant vaccine. Tehran University of Medical Sciences 2015-04 /pmc/articles/PMC4662783/ /pubmed/26622968 Text en © Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Kumar, Sujeet
Chaturvedi, Vinod Kumar
Kumar, Bablu
Kumar, Pankaj
Somarajan, Sudha Rani
Mishra, Anil Kumar
Sharma, Bhaskar
Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice
title Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice
title_full Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice
title_fullStr Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice
title_full_unstemmed Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice
title_short Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice
title_sort effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662783/
https://www.ncbi.nlm.nih.gov/pubmed/26622968
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