Cargando…
Cytotoxicity of glass ionomer cements containing silver nanoparticles
BACKGROUND: Some studies have investigated the possibility of incorporating silver nanoparticles (NAg) into dental materials to improve their antibacterial properties. However, the potential toxic effect of this material on pulp cells should be investigated in order to avoid additional damage to the...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medicina Oral S.L.
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663065/ https://www.ncbi.nlm.nih.gov/pubmed/26644839 http://dx.doi.org/10.4317/jced.52566 |
_version_ | 1782403245221609472 |
---|---|
author | Siqueira, Patrícia-Correia Magalhães, Ana-Paula-Rodrigues Pires, Wanessa-Carvalho Pereira, Flávia-Castro Silveira-Lacerda, Elisângela-Paula Carrião, Marcus-Santos Bakuzis, Andris-Figueiroa Souza-Costa, Carlos-Alberto Lopes, Lawrence-Gonzaga Estrela, Carlos |
author_facet | Siqueira, Patrícia-Correia Magalhães, Ana-Paula-Rodrigues Pires, Wanessa-Carvalho Pereira, Flávia-Castro Silveira-Lacerda, Elisângela-Paula Carrião, Marcus-Santos Bakuzis, Andris-Figueiroa Souza-Costa, Carlos-Alberto Lopes, Lawrence-Gonzaga Estrela, Carlos |
author_sort | Siqueira, Patrícia-Correia |
collection | PubMed |
description | BACKGROUND: Some studies have investigated the possibility of incorporating silver nanoparticles (NAg) into dental materials to improve their antibacterial properties. However, the potential toxic effect of this material on pulp cells should be investigated in order to avoid additional damage to the pulp tissue. This study evaluated the cytotoxicity of conventional and resin-modified glass ionomer cements (GIC) with and without addition of NAg. MATERIAL AND METHODS: NAg were added to the materials at two different concentrations by weight: 0.1% and 0.2%. Specimens with standardized dimensions were prepared, immersed in 400 µL of culture medium and incubated at 37°C and 5% CO2 for 48 h to prepare GIC liquid extracts, which were then incubated in contact with cells for 48 h. Culture medium and 0.78% NAg solution were used as negative and positive controls, respectively. Cell viability was determined by MTT and Trypan Blue assays. ANOVA and the Tukey test (α=0.05) were used for statistical analyses. RESULTS: Both tests revealed a significant decrease in cell viability in all groups of resin modified cements (p<0.001). There were no statistically significant differences between groups with and without NAg (p>0.05). The differences in cell viability between any group of conventional GIC and the negative control were not statistically significant (p>0.05). CONCLUSIONS: NAg did not affect the cytotoxicity of the GIC under evaluation. Key words:Glass ionomer cements, totoxicity, cell culture techniques, nanotechnology, metal nanoparticles. |
format | Online Article Text |
id | pubmed-4663065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medicina Oral S.L. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46630652015-12-07 Cytotoxicity of glass ionomer cements containing silver nanoparticles Siqueira, Patrícia-Correia Magalhães, Ana-Paula-Rodrigues Pires, Wanessa-Carvalho Pereira, Flávia-Castro Silveira-Lacerda, Elisângela-Paula Carrião, Marcus-Santos Bakuzis, Andris-Figueiroa Souza-Costa, Carlos-Alberto Lopes, Lawrence-Gonzaga Estrela, Carlos J Clin Exp Dent Research BACKGROUND: Some studies have investigated the possibility of incorporating silver nanoparticles (NAg) into dental materials to improve their antibacterial properties. However, the potential toxic effect of this material on pulp cells should be investigated in order to avoid additional damage to the pulp tissue. This study evaluated the cytotoxicity of conventional and resin-modified glass ionomer cements (GIC) with and without addition of NAg. MATERIAL AND METHODS: NAg were added to the materials at two different concentrations by weight: 0.1% and 0.2%. Specimens with standardized dimensions were prepared, immersed in 400 µL of culture medium and incubated at 37°C and 5% CO2 for 48 h to prepare GIC liquid extracts, which were then incubated in contact with cells for 48 h. Culture medium and 0.78% NAg solution were used as negative and positive controls, respectively. Cell viability was determined by MTT and Trypan Blue assays. ANOVA and the Tukey test (α=0.05) were used for statistical analyses. RESULTS: Both tests revealed a significant decrease in cell viability in all groups of resin modified cements (p<0.001). There were no statistically significant differences between groups with and without NAg (p>0.05). The differences in cell viability between any group of conventional GIC and the negative control were not statistically significant (p>0.05). CONCLUSIONS: NAg did not affect the cytotoxicity of the GIC under evaluation. Key words:Glass ionomer cements, totoxicity, cell culture techniques, nanotechnology, metal nanoparticles. Medicina Oral S.L. 2015-12-01 /pmc/articles/PMC4663065/ /pubmed/26644839 http://dx.doi.org/10.4317/jced.52566 Text en Copyright: © 2015 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Siqueira, Patrícia-Correia Magalhães, Ana-Paula-Rodrigues Pires, Wanessa-Carvalho Pereira, Flávia-Castro Silveira-Lacerda, Elisângela-Paula Carrião, Marcus-Santos Bakuzis, Andris-Figueiroa Souza-Costa, Carlos-Alberto Lopes, Lawrence-Gonzaga Estrela, Carlos Cytotoxicity of glass ionomer cements containing silver nanoparticles |
title | Cytotoxicity of glass ionomer cements containing silver nanoparticles |
title_full | Cytotoxicity of glass ionomer cements containing silver nanoparticles |
title_fullStr | Cytotoxicity of glass ionomer cements containing silver nanoparticles |
title_full_unstemmed | Cytotoxicity of glass ionomer cements containing silver nanoparticles |
title_short | Cytotoxicity of glass ionomer cements containing silver nanoparticles |
title_sort | cytotoxicity of glass ionomer cements containing silver nanoparticles |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663065/ https://www.ncbi.nlm.nih.gov/pubmed/26644839 http://dx.doi.org/10.4317/jced.52566 |
work_keys_str_mv | AT siqueirapatriciacorreia cytotoxicityofglassionomercementscontainingsilvernanoparticles AT magalhaesanapaularodrigues cytotoxicityofglassionomercementscontainingsilvernanoparticles AT pireswanessacarvalho cytotoxicityofglassionomercementscontainingsilvernanoparticles AT pereiraflaviacastro cytotoxicityofglassionomercementscontainingsilvernanoparticles AT silveiralacerdaelisangelapaula cytotoxicityofglassionomercementscontainingsilvernanoparticles AT carriaomarcussantos cytotoxicityofglassionomercementscontainingsilvernanoparticles AT bakuzisandrisfigueiroa cytotoxicityofglassionomercementscontainingsilvernanoparticles AT souzacostacarlosalberto cytotoxicityofglassionomercementscontainingsilvernanoparticles AT lopeslawrencegonzaga cytotoxicityofglassionomercementscontainingsilvernanoparticles AT estrelacarlos cytotoxicityofglassionomercementscontainingsilvernanoparticles |