Cargando…

Characterisation of Lipid Changes in Ethylene-Promoted Senescence and Its Retardation by Suppression of Phospholipase Dδ in Arabidopsis Leaves

Ethylene and abscisic acid (ABA) both accelerate senescence of detached Arabidopsis leaves. We previously showed that suppression of Phospholipase Dδ (PLDδ) retarded ABA-promoted senescence. Here, we report that ethylene-promoted senescence is retarded in detached leaves lacking PLDδ. We further use...

Descripción completa

Detalles Bibliográficos
Autores principales: Jia, Yanxia, Li, Weiqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663248/
https://www.ncbi.nlm.nih.gov/pubmed/26648950
http://dx.doi.org/10.3389/fpls.2015.01045
_version_ 1782403259823030272
author Jia, Yanxia
Li, Weiqi
author_facet Jia, Yanxia
Li, Weiqi
author_sort Jia, Yanxia
collection PubMed
description Ethylene and abscisic acid (ABA) both accelerate senescence of detached Arabidopsis leaves. We previously showed that suppression of Phospholipase Dδ (PLDδ) retarded ABA-promoted senescence. Here, we report that ethylene-promoted senescence is retarded in detached leaves lacking PLDδ. We further used lipidomics to comparatively profile the molecular species of membrane lipids between wild-type and PLDδ-knockout (PLDδ-KO) Arabidopsis during ethylene-promoted senescence. Lipid profiling revealed that ethylene caused a decrease in all lipids levels, except phosphatidic acid (PA), caused increases in the ratios of digalactosyl diglyceride/monogalactosyl diglyceride (MGDG) and phosphatidylcholine (PC)/phosphatidylethanolamine (PE), and caused degradation of plastidic lipids before that of extraplastidic lipids in wild-type plants. The accelerated degradation of plastidic lipids during ethylene-promoted senescence in wild-type plants was attenuated in PLDδ-KO plants. No obvious differences in substrate and product of PLDδ-catalyzed phospholipid hydrolysis were detected between wild-type and PLDδ-KO plants, which indicated that the retardation of ethylene-promoted senescence by suppressing PLDδ might not be related to the role of PLDδ in catalyzing phospholipid degradation. In contrast, higher plastidic lipid content, especially of MGDG, in PLDδ-KO plants was crucial for maintaining photosynthetic activity. The lower relative content of PA and higher PC/PE ratio in PLDδ-KO plants might contribute to maintaining cell membrane integrity. The integrity of the cell membrane in PLDδ-KO plants facilitated maintenance of the membrane function and of the proteins associated with the membrane. Taking these findings together, higher plastidic lipid content and the integrity of the cell membrane in PLDδ-KO plants might contribute to the retardation of ethylene-promoted senescence by the suppression of PLDδ.
format Online
Article
Text
id pubmed-4663248
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-46632482015-12-08 Characterisation of Lipid Changes in Ethylene-Promoted Senescence and Its Retardation by Suppression of Phospholipase Dδ in Arabidopsis Leaves Jia, Yanxia Li, Weiqi Front Plant Sci Plant Science Ethylene and abscisic acid (ABA) both accelerate senescence of detached Arabidopsis leaves. We previously showed that suppression of Phospholipase Dδ (PLDδ) retarded ABA-promoted senescence. Here, we report that ethylene-promoted senescence is retarded in detached leaves lacking PLDδ. We further used lipidomics to comparatively profile the molecular species of membrane lipids between wild-type and PLDδ-knockout (PLDδ-KO) Arabidopsis during ethylene-promoted senescence. Lipid profiling revealed that ethylene caused a decrease in all lipids levels, except phosphatidic acid (PA), caused increases in the ratios of digalactosyl diglyceride/monogalactosyl diglyceride (MGDG) and phosphatidylcholine (PC)/phosphatidylethanolamine (PE), and caused degradation of plastidic lipids before that of extraplastidic lipids in wild-type plants. The accelerated degradation of plastidic lipids during ethylene-promoted senescence in wild-type plants was attenuated in PLDδ-KO plants. No obvious differences in substrate and product of PLDδ-catalyzed phospholipid hydrolysis were detected between wild-type and PLDδ-KO plants, which indicated that the retardation of ethylene-promoted senescence by suppressing PLDδ might not be related to the role of PLDδ in catalyzing phospholipid degradation. In contrast, higher plastidic lipid content, especially of MGDG, in PLDδ-KO plants was crucial for maintaining photosynthetic activity. The lower relative content of PA and higher PC/PE ratio in PLDδ-KO plants might contribute to maintaining cell membrane integrity. The integrity of the cell membrane in PLDδ-KO plants facilitated maintenance of the membrane function and of the proteins associated with the membrane. Taking these findings together, higher plastidic lipid content and the integrity of the cell membrane in PLDδ-KO plants might contribute to the retardation of ethylene-promoted senescence by the suppression of PLDδ. Frontiers Media S.A. 2015-11-30 /pmc/articles/PMC4663248/ /pubmed/26648950 http://dx.doi.org/10.3389/fpls.2015.01045 Text en Copyright © 2015 Jia and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Jia, Yanxia
Li, Weiqi
Characterisation of Lipid Changes in Ethylene-Promoted Senescence and Its Retardation by Suppression of Phospholipase Dδ in Arabidopsis Leaves
title Characterisation of Lipid Changes in Ethylene-Promoted Senescence and Its Retardation by Suppression of Phospholipase Dδ in Arabidopsis Leaves
title_full Characterisation of Lipid Changes in Ethylene-Promoted Senescence and Its Retardation by Suppression of Phospholipase Dδ in Arabidopsis Leaves
title_fullStr Characterisation of Lipid Changes in Ethylene-Promoted Senescence and Its Retardation by Suppression of Phospholipase Dδ in Arabidopsis Leaves
title_full_unstemmed Characterisation of Lipid Changes in Ethylene-Promoted Senescence and Its Retardation by Suppression of Phospholipase Dδ in Arabidopsis Leaves
title_short Characterisation of Lipid Changes in Ethylene-Promoted Senescence and Its Retardation by Suppression of Phospholipase Dδ in Arabidopsis Leaves
title_sort characterisation of lipid changes in ethylene-promoted senescence and its retardation by suppression of phospholipase dδ in arabidopsis leaves
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663248/
https://www.ncbi.nlm.nih.gov/pubmed/26648950
http://dx.doi.org/10.3389/fpls.2015.01045
work_keys_str_mv AT jiayanxia characterisationoflipidchangesinethylenepromotedsenescenceanditsretardationbysuppressionofphospholipaseddinarabidopsisleaves
AT liweiqi characterisationoflipidchangesinethylenepromotedsenescenceanditsretardationbysuppressionofphospholipaseddinarabidopsisleaves