Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population

DNA oxidative lesions are widely considered as a potential risk factor for colorectal cancer development. The aim of this work was to determine the role of the efficiency of base excision repair, both in lymphocytes and in epithelial tissue, in patients with CRC and healthy subjects. SNPs were ident...

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Autores principales: Kabzinski, J., Mucha, B., Cuchra, M., Markiewicz, L., Przybylowska, K., Dziki, A., Dziki, L., Majsterek, I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663340/
https://www.ncbi.nlm.nih.gov/pubmed/26649135
http://dx.doi.org/10.1155/2016/3125989
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author Kabzinski, J.
Mucha, B.
Cuchra, M.
Markiewicz, L.
Przybylowska, K.
Dziki, A.
Dziki, L.
Majsterek, I.
author_facet Kabzinski, J.
Mucha, B.
Cuchra, M.
Markiewicz, L.
Przybylowska, K.
Dziki, A.
Dziki, L.
Majsterek, I.
author_sort Kabzinski, J.
collection PubMed
description DNA oxidative lesions are widely considered as a potential risk factor for colorectal cancer development. The aim of this work was to determine the role of the efficiency of base excision repair, both in lymphocytes and in epithelial tissue, in patients with CRC and healthy subjects. SNPs were identified within genes responsible for steps following glycosylase action in BER, and patients and healthy subjects were genotyped. A radioisotopic BER assay was used for assessing repair efficiency and TaqMan for genotyping. Decreased BER activity was observed in lymphocyte extract from CRC patients and in cancer tissue extract, compared to healthy subjects. In addition, polymorphisms of EXO1, LIG3, and PolB may modulate the risk of colorectal cancer by decreasing (PolB) or increasing (LIG3 and EXO1) the chance of malignant transformation.
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spelling pubmed-46633402015-12-08 Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population Kabzinski, J. Mucha, B. Cuchra, M. Markiewicz, L. Przybylowska, K. Dziki, A. Dziki, L. Majsterek, I. Oxid Med Cell Longev Research Article DNA oxidative lesions are widely considered as a potential risk factor for colorectal cancer development. The aim of this work was to determine the role of the efficiency of base excision repair, both in lymphocytes and in epithelial tissue, in patients with CRC and healthy subjects. SNPs were identified within genes responsible for steps following glycosylase action in BER, and patients and healthy subjects were genotyped. A radioisotopic BER assay was used for assessing repair efficiency and TaqMan for genotyping. Decreased BER activity was observed in lymphocyte extract from CRC patients and in cancer tissue extract, compared to healthy subjects. In addition, polymorphisms of EXO1, LIG3, and PolB may modulate the risk of colorectal cancer by decreasing (PolB) or increasing (LIG3 and EXO1) the chance of malignant transformation. Hindawi Publishing Corporation 2016 2015-11-16 /pmc/articles/PMC4663340/ /pubmed/26649135 http://dx.doi.org/10.1155/2016/3125989 Text en Copyright © 2016 J. Kabzinski et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kabzinski, J.
Mucha, B.
Cuchra, M.
Markiewicz, L.
Przybylowska, K.
Dziki, A.
Dziki, L.
Majsterek, I.
Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population
title Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population
title_full Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population
title_fullStr Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population
title_full_unstemmed Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population
title_short Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population
title_sort efficiency of base excision repair of oxidative dna damage and its impact on the risk of colorectal cancer in the polish population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663340/
https://www.ncbi.nlm.nih.gov/pubmed/26649135
http://dx.doi.org/10.1155/2016/3125989
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