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Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulato...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663344/ https://www.ncbi.nlm.nih.gov/pubmed/26649045 http://dx.doi.org/10.1155/2016/1628352 |
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author | Zhang, Lei Dang, Rui-Jie Yang, Yan-Mei Cui, Dian-Chao Li, Ping Ni, Yan-Li Hao, Tong Wang, Changyong Jiang, Xiao-Xia Fang, Nan-Zhu |
author_facet | Zhang, Lei Dang, Rui-Jie Yang, Yan-Mei Cui, Dian-Chao Li, Ping Ni, Yan-Li Hao, Tong Wang, Changyong Jiang, Xiao-Xia Fang, Nan-Zhu |
author_sort | Zhang, Lei |
collection | PubMed |
description | As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development. |
format | Online Article Text |
id | pubmed-4663344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46633442015-12-08 Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells Zhang, Lei Dang, Rui-Jie Yang, Yan-Mei Cui, Dian-Chao Li, Ping Ni, Yan-Li Hao, Tong Wang, Changyong Jiang, Xiao-Xia Fang, Nan-Zhu Stem Cells Int Research Article As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development. Hindawi Publishing Corporation 2016 2015-11-16 /pmc/articles/PMC4663344/ /pubmed/26649045 http://dx.doi.org/10.1155/2016/1628352 Text en Copyright © 2016 Lei Zhang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Lei Dang, Rui-Jie Yang, Yan-Mei Cui, Dian-Chao Li, Ping Ni, Yan-Li Hao, Tong Wang, Changyong Jiang, Xiao-Xia Fang, Nan-Zhu Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
title | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
title_full | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
title_fullStr | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
title_full_unstemmed | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
title_short | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
title_sort | delta-like-1 changes the immunomodulatory property of op9 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663344/ https://www.ncbi.nlm.nih.gov/pubmed/26649045 http://dx.doi.org/10.1155/2016/1628352 |
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