QUANTITY: An Isobaric Tag for Quantitative Glycomics

Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics - analysis of glycans at global level - however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As...

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Autores principales: Yang, Shuang, Wang, Meiyao, Chen, Lijun, Yin, Bojiao, Song, Guoqiang, Turko, Illarion V., Phinney, Karen W., Betenbaugh, Michael J., Zhang, Hui, Li, Shuwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663469/
https://www.ncbi.nlm.nih.gov/pubmed/26616285
http://dx.doi.org/10.1038/srep17585
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author Yang, Shuang
Wang, Meiyao
Chen, Lijun
Yin, Bojiao
Song, Guoqiang
Turko, Illarion V.
Phinney, Karen W.
Betenbaugh, Michael J.
Zhang, Hui
Li, Shuwei
author_facet Yang, Shuang
Wang, Meiyao
Chen, Lijun
Yin, Bojiao
Song, Guoqiang
Turko, Illarion V.
Phinney, Karen W.
Betenbaugh, Michael J.
Zhang, Hui
Li, Shuwei
author_sort Yang, Shuang
collection PubMed
description Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics - analysis of glycans at global level - however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.
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spelling pubmed-46634692015-12-03 QUANTITY: An Isobaric Tag for Quantitative Glycomics Yang, Shuang Wang, Meiyao Chen, Lijun Yin, Bojiao Song, Guoqiang Turko, Illarion V. Phinney, Karen W. Betenbaugh, Michael J. Zhang, Hui Li, Shuwei Sci Rep Article Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics - analysis of glycans at global level - however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering. Nature Publishing Group 2015-11-30 /pmc/articles/PMC4663469/ /pubmed/26616285 http://dx.doi.org/10.1038/srep17585 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yang, Shuang
Wang, Meiyao
Chen, Lijun
Yin, Bojiao
Song, Guoqiang
Turko, Illarion V.
Phinney, Karen W.
Betenbaugh, Michael J.
Zhang, Hui
Li, Shuwei
QUANTITY: An Isobaric Tag for Quantitative Glycomics
title QUANTITY: An Isobaric Tag for Quantitative Glycomics
title_full QUANTITY: An Isobaric Tag for Quantitative Glycomics
title_fullStr QUANTITY: An Isobaric Tag for Quantitative Glycomics
title_full_unstemmed QUANTITY: An Isobaric Tag for Quantitative Glycomics
title_short QUANTITY: An Isobaric Tag for Quantitative Glycomics
title_sort quantity: an isobaric tag for quantitative glycomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663469/
https://www.ncbi.nlm.nih.gov/pubmed/26616285
http://dx.doi.org/10.1038/srep17585
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