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Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia

Oxygen therapy to maintain tissue oxygenation is one of the cornerstones of critical care. Therefore, hyperoxia is often encountered in critically ill patients. Epidemiologic studies have demonstrated that hyperoxia may affect outcome, although mechanisms are unclear. Immunologic effects might be in...

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Autores principales: Kiers, Dorien, Gerretsen, Jelle, Janssen, Emmy, John, Aaron, Groeneveld, R., van der Hoeven, Johannes G., Scheffer, Gert-Jan, Pickkers, Peter, Kox, Matthijs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663498/
https://www.ncbi.nlm.nih.gov/pubmed/26616217
http://dx.doi.org/10.1038/srep17441
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author Kiers, Dorien
Gerretsen, Jelle
Janssen, Emmy
John, Aaron
Groeneveld, R.
van der Hoeven, Johannes G.
Scheffer, Gert-Jan
Pickkers, Peter
Kox, Matthijs
author_facet Kiers, Dorien
Gerretsen, Jelle
Janssen, Emmy
John, Aaron
Groeneveld, R.
van der Hoeven, Johannes G.
Scheffer, Gert-Jan
Pickkers, Peter
Kox, Matthijs
author_sort Kiers, Dorien
collection PubMed
description Oxygen therapy to maintain tissue oxygenation is one of the cornerstones of critical care. Therefore, hyperoxia is often encountered in critically ill patients. Epidemiologic studies have demonstrated that hyperoxia may affect outcome, although mechanisms are unclear. Immunologic effects might be involved, as hyperoxia was shown to attenuate inflammation and organ damage in preclinical models. However, it remains unclear whether these observations can be ascribed to direct immunosuppressive effects of hyperoxia or to preserved tissue oxygenation. In contrast to these putative anti-inflammatory effects, hyperoxia may elicit an inflammatory response and organ damage in itself, known as oxygen toxicity. Here, we demonstrate that, in the absence of systemic inflammation, short-term hyperoxia (100% O(2) for 2.5 hours in mice and 3.5 hours in humans) does not result in increased levels of inflammatory cytokines in both mice and healthy volunteers. Furthermore, we show that, compared with room air, hyperoxia does not affect the systemic inflammatory response elicited by administration of bacterial endotoxin in mice and man. Finally, neutrophil phagocytosis and ROS generation are unaffected by short-term hyperoxia. Our results indicate that hyperoxia does not exert direct anti-inflammatory effects and temper expectations of using it as an immunomodulatory treatment strategy.
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spelling pubmed-46634982015-12-03 Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia Kiers, Dorien Gerretsen, Jelle Janssen, Emmy John, Aaron Groeneveld, R. van der Hoeven, Johannes G. Scheffer, Gert-Jan Pickkers, Peter Kox, Matthijs Sci Rep Article Oxygen therapy to maintain tissue oxygenation is one of the cornerstones of critical care. Therefore, hyperoxia is often encountered in critically ill patients. Epidemiologic studies have demonstrated that hyperoxia may affect outcome, although mechanisms are unclear. Immunologic effects might be involved, as hyperoxia was shown to attenuate inflammation and organ damage in preclinical models. However, it remains unclear whether these observations can be ascribed to direct immunosuppressive effects of hyperoxia or to preserved tissue oxygenation. In contrast to these putative anti-inflammatory effects, hyperoxia may elicit an inflammatory response and organ damage in itself, known as oxygen toxicity. Here, we demonstrate that, in the absence of systemic inflammation, short-term hyperoxia (100% O(2) for 2.5 hours in mice and 3.5 hours in humans) does not result in increased levels of inflammatory cytokines in both mice and healthy volunteers. Furthermore, we show that, compared with room air, hyperoxia does not affect the systemic inflammatory response elicited by administration of bacterial endotoxin in mice and man. Finally, neutrophil phagocytosis and ROS generation are unaffected by short-term hyperoxia. Our results indicate that hyperoxia does not exert direct anti-inflammatory effects and temper expectations of using it as an immunomodulatory treatment strategy. Nature Publishing Group 2015-11-30 /pmc/articles/PMC4663498/ /pubmed/26616217 http://dx.doi.org/10.1038/srep17441 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kiers, Dorien
Gerretsen, Jelle
Janssen, Emmy
John, Aaron
Groeneveld, R.
van der Hoeven, Johannes G.
Scheffer, Gert-Jan
Pickkers, Peter
Kox, Matthijs
Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia
title Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia
title_full Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia
title_fullStr Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia
title_full_unstemmed Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia
title_short Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia
title_sort short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663498/
https://www.ncbi.nlm.nih.gov/pubmed/26616217
http://dx.doi.org/10.1038/srep17441
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