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Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI

CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in atherosclerosis have shown that CD163 expression is increased at inflammatory sites, pointing at the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging of CD163 expressing macrophages is...

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Autores principales: Tarin, Carlos, Carril, Monica, Martin-Ventura, Jose Luis, Markuerkiaga, Irati, Padro, Daniel, Llamas-Granda, Patricia, Moreno, Juan Antonio, García, Isabel, Genicio, Nuria, Plaza-Garcia, Sandra, Blanco-Colio, Luis Miguel, Penades, Soledad, Egido, Jesus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663748/
https://www.ncbi.nlm.nih.gov/pubmed/26616677
http://dx.doi.org/10.1038/srep17135
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author Tarin, Carlos
Carril, Monica
Martin-Ventura, Jose Luis
Markuerkiaga, Irati
Padro, Daniel
Llamas-Granda, Patricia
Moreno, Juan Antonio
García, Isabel
Genicio, Nuria
Plaza-Garcia, Sandra
Blanco-Colio, Luis Miguel
Penades, Soledad
Egido, Jesus
author_facet Tarin, Carlos
Carril, Monica
Martin-Ventura, Jose Luis
Markuerkiaga, Irati
Padro, Daniel
Llamas-Granda, Patricia
Moreno, Juan Antonio
García, Isabel
Genicio, Nuria
Plaza-Garcia, Sandra
Blanco-Colio, Luis Miguel
Penades, Soledad
Egido, Jesus
author_sort Tarin, Carlos
collection PubMed
description CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in atherosclerosis have shown that CD163 expression is increased at inflammatory sites, pointing at the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging of CD163 expressing macrophages is an interesting strategy in order to detect atherosclerotic plaques. We have prepared a targeted probe based on gold-coated iron oxide nanoparticles vectorized with an anti-CD163 antibody for the specific detection of CD163 by MRI. Firstly, the specificity of the targeted probe was validated in vitro by incubation of the probe with CD163(+) or (−) macrophages. The probe was able to selectively detect CD163(+) macrophages both in human and murine cells. Subsequently, the targeted probe was injected in 16 weeks old apoE deficient mice developing atherosclerotic lesions and the pararenal abdominal aorta was imaged by MRI. The accumulation of probe in the site of interest increased over time and the signal intensity decreased significantly 48 hours after the injection. Hence, we have developed a highly sensitive targeted probe capable of detecting CD163-expressing macrophages that could provide useful information about the state of the atheromatous lesions.
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spelling pubmed-46637482015-12-03 Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI Tarin, Carlos Carril, Monica Martin-Ventura, Jose Luis Markuerkiaga, Irati Padro, Daniel Llamas-Granda, Patricia Moreno, Juan Antonio García, Isabel Genicio, Nuria Plaza-Garcia, Sandra Blanco-Colio, Luis Miguel Penades, Soledad Egido, Jesus Sci Rep Article CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in atherosclerosis have shown that CD163 expression is increased at inflammatory sites, pointing at the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging of CD163 expressing macrophages is an interesting strategy in order to detect atherosclerotic plaques. We have prepared a targeted probe based on gold-coated iron oxide nanoparticles vectorized with an anti-CD163 antibody for the specific detection of CD163 by MRI. Firstly, the specificity of the targeted probe was validated in vitro by incubation of the probe with CD163(+) or (−) macrophages. The probe was able to selectively detect CD163(+) macrophages both in human and murine cells. Subsequently, the targeted probe was injected in 16 weeks old apoE deficient mice developing atherosclerotic lesions and the pararenal abdominal aorta was imaged by MRI. The accumulation of probe in the site of interest increased over time and the signal intensity decreased significantly 48 hours after the injection. Hence, we have developed a highly sensitive targeted probe capable of detecting CD163-expressing macrophages that could provide useful information about the state of the atheromatous lesions. Nature Publishing Group 2015-11-30 /pmc/articles/PMC4663748/ /pubmed/26616677 http://dx.doi.org/10.1038/srep17135 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tarin, Carlos
Carril, Monica
Martin-Ventura, Jose Luis
Markuerkiaga, Irati
Padro, Daniel
Llamas-Granda, Patricia
Moreno, Juan Antonio
García, Isabel
Genicio, Nuria
Plaza-Garcia, Sandra
Blanco-Colio, Luis Miguel
Penades, Soledad
Egido, Jesus
Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI
title Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI
title_full Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI
title_fullStr Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI
title_full_unstemmed Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI
title_short Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI
title_sort targeted gold-coated iron oxide nanoparticles for cd163 detection in atherosclerosis by mri
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663748/
https://www.ncbi.nlm.nih.gov/pubmed/26616677
http://dx.doi.org/10.1038/srep17135
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