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Exposure to Palladium Nanoparticles Affects Serum Levels of Cytokines in Female Wistar Rats
BACKGROUND: Information currently available on the impact of palladium on the immune system mainly derives from studies assessing the biological effects of palladium salts. However, in the last years, there has been a notable increase in occupational and environmental levels of fine and ultrafine pa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664404/ https://www.ncbi.nlm.nih.gov/pubmed/26618704 http://dx.doi.org/10.1371/journal.pone.0143801 |
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author | Iavicoli, Ivo Fontana, Luca Corbi, Maddalena Leso, Veruscka Marinaccio, Alessandro Leopold, Kerstin Schindl, Roland Sgambato, Alessandro |
author_facet | Iavicoli, Ivo Fontana, Luca Corbi, Maddalena Leso, Veruscka Marinaccio, Alessandro Leopold, Kerstin Schindl, Roland Sgambato, Alessandro |
author_sort | Iavicoli, Ivo |
collection | PubMed |
description | BACKGROUND: Information currently available on the impact of palladium on the immune system mainly derives from studies assessing the biological effects of palladium salts. However, in the last years, there has been a notable increase in occupational and environmental levels of fine and ultrafine palladium particles released from automobile catalytic converters, which may play a role in palladium sensitization. In this context, the evaluation of the possible effects exerted by palladium nanoparticles (Pd-NPs) on the immune system is essential to comprehensively assess palladium immunotoxic potential. AIM: Therefore, the aim of this study was to investigate the effects of Pd-NPs on the immune system of female Wistar rats exposed to this xenobiotic for 14 days, by assessing possible quantitative changes in a number of cytokines: IL-1α, IL-2, IL-4, IL-6, IL-10, IL-12, GM-CSF, INF-γ and TNF-α. METHODS: Twenty rats were randomly divided into four exposure groups and one of control. Animals were given a single tail vein injection of vehicle (control group) and different concentrations of Pd-NPs (0.012, 0.12, 1.2 and 12 μg/kg). A multiplex biometric enzyme linked immunosorbent assay was used to evaluate cytokine serum levels. RESULTS: The mean serum concentrations of all cytokines decreased after the administration of 0.012 μg/kg of Pd-NPs, whereas exceeded the control levels at higher exposure doses. The highest concentration of Pd-NPs (12 μg/kg) induced a significant increase of IL-1α, IL-4, IL-6, IL-10, IL-12, GM-CSF and INF-γ compared to controls. DISCUSSION AND CONCLUSIONS: These results demonstrated that Pd-NP exposure can affect the immune response of rats inducing a stimulatory action that becomes significant at the highest administered dose. Our findings did not show an imbalance between cytokines produced by CD4(+) T helper (Th) cells 1 and 2, thus suggesting a generalized stimulation of the immune system with a simultaneous activation and polarization of the naïve T cells towards Th1 and Th2 phenotype. |
format | Online Article Text |
id | pubmed-4664404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46644042015-12-10 Exposure to Palladium Nanoparticles Affects Serum Levels of Cytokines in Female Wistar Rats Iavicoli, Ivo Fontana, Luca Corbi, Maddalena Leso, Veruscka Marinaccio, Alessandro Leopold, Kerstin Schindl, Roland Sgambato, Alessandro PLoS One Research Article BACKGROUND: Information currently available on the impact of palladium on the immune system mainly derives from studies assessing the biological effects of palladium salts. However, in the last years, there has been a notable increase in occupational and environmental levels of fine and ultrafine palladium particles released from automobile catalytic converters, which may play a role in palladium sensitization. In this context, the evaluation of the possible effects exerted by palladium nanoparticles (Pd-NPs) on the immune system is essential to comprehensively assess palladium immunotoxic potential. AIM: Therefore, the aim of this study was to investigate the effects of Pd-NPs on the immune system of female Wistar rats exposed to this xenobiotic for 14 days, by assessing possible quantitative changes in a number of cytokines: IL-1α, IL-2, IL-4, IL-6, IL-10, IL-12, GM-CSF, INF-γ and TNF-α. METHODS: Twenty rats were randomly divided into four exposure groups and one of control. Animals were given a single tail vein injection of vehicle (control group) and different concentrations of Pd-NPs (0.012, 0.12, 1.2 and 12 μg/kg). A multiplex biometric enzyme linked immunosorbent assay was used to evaluate cytokine serum levels. RESULTS: The mean serum concentrations of all cytokines decreased after the administration of 0.012 μg/kg of Pd-NPs, whereas exceeded the control levels at higher exposure doses. The highest concentration of Pd-NPs (12 μg/kg) induced a significant increase of IL-1α, IL-4, IL-6, IL-10, IL-12, GM-CSF and INF-γ compared to controls. DISCUSSION AND CONCLUSIONS: These results demonstrated that Pd-NP exposure can affect the immune response of rats inducing a stimulatory action that becomes significant at the highest administered dose. Our findings did not show an imbalance between cytokines produced by CD4(+) T helper (Th) cells 1 and 2, thus suggesting a generalized stimulation of the immune system with a simultaneous activation and polarization of the naïve T cells towards Th1 and Th2 phenotype. Public Library of Science 2015-11-30 /pmc/articles/PMC4664404/ /pubmed/26618704 http://dx.doi.org/10.1371/journal.pone.0143801 Text en © 2015 Iavicoli et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Iavicoli, Ivo Fontana, Luca Corbi, Maddalena Leso, Veruscka Marinaccio, Alessandro Leopold, Kerstin Schindl, Roland Sgambato, Alessandro Exposure to Palladium Nanoparticles Affects Serum Levels of Cytokines in Female Wistar Rats |
title | Exposure to Palladium Nanoparticles Affects Serum Levels of Cytokines in Female Wistar Rats |
title_full | Exposure to Palladium Nanoparticles Affects Serum Levels of Cytokines in Female Wistar Rats |
title_fullStr | Exposure to Palladium Nanoparticles Affects Serum Levels of Cytokines in Female Wistar Rats |
title_full_unstemmed | Exposure to Palladium Nanoparticles Affects Serum Levels of Cytokines in Female Wistar Rats |
title_short | Exposure to Palladium Nanoparticles Affects Serum Levels of Cytokines in Female Wistar Rats |
title_sort | exposure to palladium nanoparticles affects serum levels of cytokines in female wistar rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664404/ https://www.ncbi.nlm.nih.gov/pubmed/26618704 http://dx.doi.org/10.1371/journal.pone.0143801 |
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