Cargando…

De novo transcriptome assembly of two different peach cultivars grown in Korea

Peach (Prunus persica) is one of the most popular stone fruits worldwide. Next generation sequencing (NGS) has facilitated genome and transcriptome analyses of several stone fruit trees. In this study, we conducted de novo transcriptome analyses of two peach cultivars grown in Korea. Leaves of two c...

Descripción completa

Detalles Bibliográficos
Autores principales: Jo, Yeonhwa, Chu, Hyosub, Cho, Jin Kyong, Choi, Hoseong, Lian, Sen, Cho, Won Kyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664764/
https://www.ncbi.nlm.nih.gov/pubmed/26697390
http://dx.doi.org/10.1016/j.gdata.2015.10.014
_version_ 1782403482402160640
author Jo, Yeonhwa
Chu, Hyosub
Cho, Jin Kyong
Choi, Hoseong
Lian, Sen
Cho, Won Kyong
author_facet Jo, Yeonhwa
Chu, Hyosub
Cho, Jin Kyong
Choi, Hoseong
Lian, Sen
Cho, Won Kyong
author_sort Jo, Yeonhwa
collection PubMed
description Peach (Prunus persica) is one of the most popular stone fruits worldwide. Next generation sequencing (NGS) has facilitated genome and transcriptome analyses of several stone fruit trees. In this study, we conducted de novo transcriptome analyses of two peach cultivars grown in Korea. Leaves of two cultivars, referred to as Jangtaek and Mibaek, were harvested and used for library preparation. The two prepared libraries were paired-end sequenced by the HiSeq2000 system. We obtained 8.14 GB and 9.62 GB sequence data from Jangtaek and Mibaek (NCBI accession numbers: SRS1056585 and SRS1056587), respectively. The Trinity program was used to assemble two transcriptomes de novo, resulting in 110,477 (Jangtaek) and 136,196 (Mibaek) transcripts. TransDecoder identified possible coding regions in assembled transcripts. The identified proteins were subjected to BLASTP search against NCBI's non-redundant database for functional annotation. This study provides transcriptome data for two peach cultivars, which might be useful for genetic marker development and comparative transcriptome analyses.
format Online
Article
Text
id pubmed-4664764
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-46647642015-12-22 De novo transcriptome assembly of two different peach cultivars grown in Korea Jo, Yeonhwa Chu, Hyosub Cho, Jin Kyong Choi, Hoseong Lian, Sen Cho, Won Kyong Genom Data Data in Brief Peach (Prunus persica) is one of the most popular stone fruits worldwide. Next generation sequencing (NGS) has facilitated genome and transcriptome analyses of several stone fruit trees. In this study, we conducted de novo transcriptome analyses of two peach cultivars grown in Korea. Leaves of two cultivars, referred to as Jangtaek and Mibaek, were harvested and used for library preparation. The two prepared libraries were paired-end sequenced by the HiSeq2000 system. We obtained 8.14 GB and 9.62 GB sequence data from Jangtaek and Mibaek (NCBI accession numbers: SRS1056585 and SRS1056587), respectively. The Trinity program was used to assemble two transcriptomes de novo, resulting in 110,477 (Jangtaek) and 136,196 (Mibaek) transcripts. TransDecoder identified possible coding regions in assembled transcripts. The identified proteins were subjected to BLASTP search against NCBI's non-redundant database for functional annotation. This study provides transcriptome data for two peach cultivars, which might be useful for genetic marker development and comparative transcriptome analyses. Elsevier 2015-10-19 /pmc/articles/PMC4664764/ /pubmed/26697390 http://dx.doi.org/10.1016/j.gdata.2015.10.014 Text en © 2015 The Authors. Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data in Brief
Jo, Yeonhwa
Chu, Hyosub
Cho, Jin Kyong
Choi, Hoseong
Lian, Sen
Cho, Won Kyong
De novo transcriptome assembly of two different peach cultivars grown in Korea
title De novo transcriptome assembly of two different peach cultivars grown in Korea
title_full De novo transcriptome assembly of two different peach cultivars grown in Korea
title_fullStr De novo transcriptome assembly of two different peach cultivars grown in Korea
title_full_unstemmed De novo transcriptome assembly of two different peach cultivars grown in Korea
title_short De novo transcriptome assembly of two different peach cultivars grown in Korea
title_sort de novo transcriptome assembly of two different peach cultivars grown in korea
topic Data in Brief
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664764/
https://www.ncbi.nlm.nih.gov/pubmed/26697390
http://dx.doi.org/10.1016/j.gdata.2015.10.014
work_keys_str_mv AT joyeonhwa denovotranscriptomeassemblyoftwodifferentpeachcultivarsgrowninkorea
AT chuhyosub denovotranscriptomeassemblyoftwodifferentpeachcultivarsgrowninkorea
AT chojinkyong denovotranscriptomeassemblyoftwodifferentpeachcultivarsgrowninkorea
AT choihoseong denovotranscriptomeassemblyoftwodifferentpeachcultivarsgrowninkorea
AT liansen denovotranscriptomeassemblyoftwodifferentpeachcultivarsgrowninkorea
AT chowonkyong denovotranscriptomeassemblyoftwodifferentpeachcultivarsgrowninkorea