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Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts
Quiescent stem cells contribute to tissue homeostasis and repair in adult mammals. We identified a tumor suppressor PRDM2, as an epigenetic regulator induced in quiescent muscle stem cells as well as in cultured quiescent myoblasts. To delineate the functions of PRDM2 in muscle cells, we compared th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664777/ https://www.ncbi.nlm.nih.gov/pubmed/26697392 http://dx.doi.org/10.1016/j.gdata.2015.10.004 |
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author | Cheedipudi, Sirisha Gala, Hardik P. Puri, Deepika Dhawan, Jyotsna |
author_facet | Cheedipudi, Sirisha Gala, Hardik P. Puri, Deepika Dhawan, Jyotsna |
author_sort | Cheedipudi, Sirisha |
collection | PubMed |
description | Quiescent stem cells contribute to tissue homeostasis and repair in adult mammals. We identified a tumor suppressor PRDM2, as an epigenetic regulator induced in quiescent muscle stem cells as well as in cultured quiescent myoblasts. To delineate the functions of PRDM2 in muscle cells, we compared the gene expression profiles of control and PRDM2 knockdown myoblasts in growing, differentiating and quiescent conditions (GEO accession number: GSE 58676). To identify the direct targets of PRDM2 and the promoters co-associated with H3K9me2 (mark catalyzed by PRDM2), ChIP-Chip analysis was performed (GSE58748). In this report we discuss in detail the methodology used to identify PRDM2 regulated genes and classify them into potential direct and indirect targets. |
format | Online Article Text |
id | pubmed-4664777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-46647772015-12-22 Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts Cheedipudi, Sirisha Gala, Hardik P. Puri, Deepika Dhawan, Jyotsna Genom Data Data in Brief Quiescent stem cells contribute to tissue homeostasis and repair in adult mammals. We identified a tumor suppressor PRDM2, as an epigenetic regulator induced in quiescent muscle stem cells as well as in cultured quiescent myoblasts. To delineate the functions of PRDM2 in muscle cells, we compared the gene expression profiles of control and PRDM2 knockdown myoblasts in growing, differentiating and quiescent conditions (GEO accession number: GSE 58676). To identify the direct targets of PRDM2 and the promoters co-associated with H3K9me2 (mark catalyzed by PRDM2), ChIP-Chip analysis was performed (GSE58748). In this report we discuss in detail the methodology used to identify PRDM2 regulated genes and classify them into potential direct and indirect targets. Elsevier 2015-10-23 /pmc/articles/PMC4664777/ /pubmed/26697392 http://dx.doi.org/10.1016/j.gdata.2015.10.004 Text en © 2015 Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Data in Brief Cheedipudi, Sirisha Gala, Hardik P. Puri, Deepika Dhawan, Jyotsna Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts |
title | Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts |
title_full | Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts |
title_fullStr | Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts |
title_full_unstemmed | Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts |
title_short | Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts |
title_sort | identification of prdm2 regulated genes in quiescent c2c12 myoblasts |
topic | Data in Brief |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664777/ https://www.ncbi.nlm.nih.gov/pubmed/26697392 http://dx.doi.org/10.1016/j.gdata.2015.10.004 |
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