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Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5

Bacteria capable of degrading polyhydroxyalkanoates (PHA) by secreting extracellular depolymerase enzymes were isolated from water and soil samples collected from various environments in Malaysia. A total of 8 potential degraders exhibited clear zones on poly(3-hydroxybutyrate) [P(3HB)] based agar,...

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Autores principales: Vigneswari, S., Lee, T. S., Bhubalan, Kesaven, Amirul, A. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664802/
https://www.ncbi.nlm.nih.gov/pubmed/26664741
http://dx.doi.org/10.1155/2015/212159
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author Vigneswari, S.
Lee, T. S.
Bhubalan, Kesaven
Amirul, A. A.
author_facet Vigneswari, S.
Lee, T. S.
Bhubalan, Kesaven
Amirul, A. A.
author_sort Vigneswari, S.
collection PubMed
description Bacteria capable of degrading polyhydroxyalkanoates (PHA) by secreting extracellular depolymerase enzymes were isolated from water and soil samples collected from various environments in Malaysia. A total of 8 potential degraders exhibited clear zones on poly(3-hydroxybutyrate) [P(3HB)] based agar, indicating the presence of extracellular PHA depolymerase. Among the isolates, DP5 exhibited the largest clearing zone with a degradation index of 6.0. The highest degradation activity of P(3HB) was also observed with depolymerase enzyme of DP5 in mineral salt medium containing P(3HB). Based on biochemical characterization and 16S rRNA cloning and sequencing, isolate DP5 was found to belong to the genus Acidovorax and subsequently named as Acidovorax sp. DP5. The highest extracellular depolymerase enzyme activity was achieved when 0.25% (w/v) of P(3HB) and 1 g/L of urea were used as carbon and nitrogen source, respectively, in the culture media. The most suitable assay condition of the depolymerase enzyme in response to pH and temperature was tested. The depolymerase produced by strain Acidovorax sp. DP5 showed high percentage of degradation with P(3HB) films in an alkaline condition with pH 9 and at a temperature of 40°C.
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spelling pubmed-46648022015-12-09 Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5 Vigneswari, S. Lee, T. S. Bhubalan, Kesaven Amirul, A. A. Enzyme Res Research Article Bacteria capable of degrading polyhydroxyalkanoates (PHA) by secreting extracellular depolymerase enzymes were isolated from water and soil samples collected from various environments in Malaysia. A total of 8 potential degraders exhibited clear zones on poly(3-hydroxybutyrate) [P(3HB)] based agar, indicating the presence of extracellular PHA depolymerase. Among the isolates, DP5 exhibited the largest clearing zone with a degradation index of 6.0. The highest degradation activity of P(3HB) was also observed with depolymerase enzyme of DP5 in mineral salt medium containing P(3HB). Based on biochemical characterization and 16S rRNA cloning and sequencing, isolate DP5 was found to belong to the genus Acidovorax and subsequently named as Acidovorax sp. DP5. The highest extracellular depolymerase enzyme activity was achieved when 0.25% (w/v) of P(3HB) and 1 g/L of urea were used as carbon and nitrogen source, respectively, in the culture media. The most suitable assay condition of the depolymerase enzyme in response to pH and temperature was tested. The depolymerase produced by strain Acidovorax sp. DP5 showed high percentage of degradation with P(3HB) films in an alkaline condition with pH 9 and at a temperature of 40°C. Hindawi Publishing Corporation 2015 2015-11-17 /pmc/articles/PMC4664802/ /pubmed/26664741 http://dx.doi.org/10.1155/2015/212159 Text en Copyright © 2015 S. Vigneswari et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vigneswari, S.
Lee, T. S.
Bhubalan, Kesaven
Amirul, A. A.
Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5
title Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5
title_full Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5
title_fullStr Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5
title_full_unstemmed Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5
title_short Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5
title_sort extracellular polyhydroxyalkanoate depolymerase by acidovorax sp. dp5
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664802/
https://www.ncbi.nlm.nih.gov/pubmed/26664741
http://dx.doi.org/10.1155/2015/212159
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