Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos

The CRISPR-Cas RNA-guided system has versatile uses in many organisms and allows modification of multiple target sites simultaneously. Generating novel genetically modified mouse and rat models is one valuable application of this system. Through the injection of Cas9 protein instead of mRNA into emb...

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Detalles Bibliográficos
Autores principales: Wang, Liren, Shao, Yanjiao, Guan, Yuting, Li, Liang, Wu, Lijuan, Chen, Fangrui, Liu, Meizhen, Chen, Huaqing, Ma, Yanlin, Ma, Xueyun, Liu, Mingyao, Li, Dali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664917/
https://www.ncbi.nlm.nih.gov/pubmed/26620761
http://dx.doi.org/10.1038/srep17517

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