Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae

In order to study the effects of Nosema ceranae infection on honey bee microRNA (miRNA) expression, we deep-sequenced honey bee miRNAs daily across a full 6-day parasite reproduction cycle. Seventeen miRNAs were differentially expressed in honey bees infected by N. ceranae that potentially target ov...

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Detalles Bibliográficos
Autores principales: Huang, Qiang, Chen, Yanping, Wang, Rui Wu, Schwarz, Ryan S., Evans, Jay D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664923/
https://www.ncbi.nlm.nih.gov/pubmed/26620304
http://dx.doi.org/10.1038/srep17494
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author Huang, Qiang
Chen, Yanping
Wang, Rui Wu
Schwarz, Ryan S.
Evans, Jay D.
author_facet Huang, Qiang
Chen, Yanping
Wang, Rui Wu
Schwarz, Ryan S.
Evans, Jay D.
author_sort Huang, Qiang
collection PubMed
description In order to study the effects of Nosema ceranae infection on honey bee microRNA (miRNA) expression, we deep-sequenced honey bee miRNAs daily across a full 6-day parasite reproduction cycle. Seventeen miRNAs were differentially expressed in honey bees infected by N. ceranae that potentially target over 400 genes predicted to primarily involve ion binding, signaling, the nucleus, transmembrane transport, and DNA binding. Based on Enzyme Code analysis, nine biological pathways were identified by screening target genes against the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, seven of which involved metabolism. Our results suggest that differentially expressed miRNAs regulate metabolism related genes of host honey bees in response to N. ceranae infection.
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spelling pubmed-46649232015-12-03 Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae Huang, Qiang Chen, Yanping Wang, Rui Wu Schwarz, Ryan S. Evans, Jay D. Sci Rep Article In order to study the effects of Nosema ceranae infection on honey bee microRNA (miRNA) expression, we deep-sequenced honey bee miRNAs daily across a full 6-day parasite reproduction cycle. Seventeen miRNAs were differentially expressed in honey bees infected by N. ceranae that potentially target over 400 genes predicted to primarily involve ion binding, signaling, the nucleus, transmembrane transport, and DNA binding. Based on Enzyme Code analysis, nine biological pathways were identified by screening target genes against the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, seven of which involved metabolism. Our results suggest that differentially expressed miRNAs regulate metabolism related genes of host honey bees in response to N. ceranae infection. Nature Publishing Group 2015-12-01 /pmc/articles/PMC4664923/ /pubmed/26620304 http://dx.doi.org/10.1038/srep17494 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Huang, Qiang
Chen, Yanping
Wang, Rui Wu
Schwarz, Ryan S.
Evans, Jay D.
Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae
title Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae
title_full Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae
title_fullStr Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae
title_full_unstemmed Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae
title_short Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae
title_sort honey bee micrornas respond to infection by the microsporidian parasite nosema ceranae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664923/
https://www.ncbi.nlm.nih.gov/pubmed/26620304
http://dx.doi.org/10.1038/srep17494
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AT wangruiwu honeybeemicrornasrespondtoinfectionbythemicrosporidianparasitenosemaceranae
AT schwarzryans honeybeemicrornasrespondtoinfectionbythemicrosporidianparasitenosemaceranae
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