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Ribosome profiling reveals an important role for translational control in circadian gene expression

Physiological and behavioral circadian rhythms are driven by a conserved transcriptional/translational negative feedback loop in mammals. Although most core clock factors are transcription factors, post-transcriptional control introduces delays that are critical for circadian oscillations. Little wo...

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Detalles Bibliográficos
Autores principales: Jang, Christopher, Lahens, Nicholas F., Hogenesch, John B., Sehgal, Amita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665005/
https://www.ncbi.nlm.nih.gov/pubmed/26338483
http://dx.doi.org/10.1101/gr.191296.115
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author Jang, Christopher
Lahens, Nicholas F.
Hogenesch, John B.
Sehgal, Amita
author_facet Jang, Christopher
Lahens, Nicholas F.
Hogenesch, John B.
Sehgal, Amita
author_sort Jang, Christopher
collection PubMed
description Physiological and behavioral circadian rhythms are driven by a conserved transcriptional/translational negative feedback loop in mammals. Although most core clock factors are transcription factors, post-transcriptional control introduces delays that are critical for circadian oscillations. Little work has been done on circadian regulation of translation, so to address this deficit we conducted ribosome profiling experiments in a human cell model for an autonomous clock. We found that most rhythmic gene expression occurs with little delay between transcription and translation, suggesting that the lag in the accumulation of some clock proteins relative to their mRNAs does not arise from regulated translation. Nevertheless, we found that translation occurs in a circadian fashion for many genes, sometimes imposing an additional level of control on rhythmically expressed mRNAs and, in other cases, conferring rhythms on noncycling mRNAs. Most cyclically transcribed RNAs are translated at one of two major times in a 24-h day, while rhythmic translation of most noncyclic RNAs is phased to a single time of day. Unexpectedly, we found that the clock also regulates the formation of cytoplasmic processing (P) bodies, which control the fate of mRNAs, suggesting circadian coordination of mRNA metabolism and translation.
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spelling pubmed-46650052016-06-01 Ribosome profiling reveals an important role for translational control in circadian gene expression Jang, Christopher Lahens, Nicholas F. Hogenesch, John B. Sehgal, Amita Genome Res Research Physiological and behavioral circadian rhythms are driven by a conserved transcriptional/translational negative feedback loop in mammals. Although most core clock factors are transcription factors, post-transcriptional control introduces delays that are critical for circadian oscillations. Little work has been done on circadian regulation of translation, so to address this deficit we conducted ribosome profiling experiments in a human cell model for an autonomous clock. We found that most rhythmic gene expression occurs with little delay between transcription and translation, suggesting that the lag in the accumulation of some clock proteins relative to their mRNAs does not arise from regulated translation. Nevertheless, we found that translation occurs in a circadian fashion for many genes, sometimes imposing an additional level of control on rhythmically expressed mRNAs and, in other cases, conferring rhythms on noncycling mRNAs. Most cyclically transcribed RNAs are translated at one of two major times in a 24-h day, while rhythmic translation of most noncyclic RNAs is phased to a single time of day. Unexpectedly, we found that the clock also regulates the formation of cytoplasmic processing (P) bodies, which control the fate of mRNAs, suggesting circadian coordination of mRNA metabolism and translation. Cold Spring Harbor Laboratory Press 2015-12 /pmc/articles/PMC4665005/ /pubmed/26338483 http://dx.doi.org/10.1101/gr.191296.115 Text en © 2015 Jang et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Jang, Christopher
Lahens, Nicholas F.
Hogenesch, John B.
Sehgal, Amita
Ribosome profiling reveals an important role for translational control in circadian gene expression
title Ribosome profiling reveals an important role for translational control in circadian gene expression
title_full Ribosome profiling reveals an important role for translational control in circadian gene expression
title_fullStr Ribosome profiling reveals an important role for translational control in circadian gene expression
title_full_unstemmed Ribosome profiling reveals an important role for translational control in circadian gene expression
title_short Ribosome profiling reveals an important role for translational control in circadian gene expression
title_sort ribosome profiling reveals an important role for translational control in circadian gene expression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665005/
https://www.ncbi.nlm.nih.gov/pubmed/26338483
http://dx.doi.org/10.1101/gr.191296.115
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