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Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis

The aim of the present study was to investigate the effect of endogenous carbon monoxide (CO) on the hydrogen sulfide/cystathionine-γ-lyase (H(2)S/CSE) pathway in cirrhotic rat livers. The rats were allocated at random into four groups: Sham, cirrhosis, cobalt protoporphyrin (CoPP) and zinc protopor...

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Autores principales: GUO, SHI-BIN, DUAN, ZHI-JUN, WANG, QIU-MING, ZHOU, QIN, LI, QING, SUN, XIAO-YU
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665341/
https://www.ncbi.nlm.nih.gov/pubmed/26668593
http://dx.doi.org/10.3892/etm.2015.2823
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author GUO, SHI-BIN
DUAN, ZHI-JUN
WANG, QIU-MING
ZHOU, QIN
LI, QING
SUN, XIAO-YU
author_facet GUO, SHI-BIN
DUAN, ZHI-JUN
WANG, QIU-MING
ZHOU, QIN
LI, QING
SUN, XIAO-YU
author_sort GUO, SHI-BIN
collection PubMed
description The aim of the present study was to investigate the effect of endogenous carbon monoxide (CO) on the hydrogen sulfide/cystathionine-γ-lyase (H(2)S/CSE) pathway in cirrhotic rat livers. The rats were allocated at random into four groups: Sham, cirrhosis, cobalt protoporphyrin (CoPP) and zinc protoporphyrin IX (ZnPP). The expression of hepatic CSE mRNA was evaluated using a quantitative polymerase chain reaction, while CSE protein expression was determined using immunohistochemical analysis. Hematoxylin and eosin staining was performed for the histological evaluation of liver fibrosis. The levels of H(2)S, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and carboxyhemoglobin (COHb) in the arterial blood were determined, in addition to the portal vein pressure. The mRNA and protein expression levels of hepatic CSE and the serum levels of H(2)S were significantly decreased in the cirrhosis group compared with those in the sham group (P<0.05). Compared with the cirrhosis group, rats in the ZnPP group had significantly lower levels of serum ALT, AST and TBIL, arterial COHb and hepatic fibrosis, while hepatic CSE expression and the production of H(2)S were significantly increased (P<0.05). The CoPP group exhibited decreased hepatic CSE expression and H(2)S production, but aggravated hepatic function and fibrosis (P<0.05). In conclusion, the H(2)S/CSE pathway is involved in the formation of liver cirrhosis and serves a crucial function in protecting liver cells against the progression of liver fibrosis. Endogenous CO downregulates hepatic CSE mRNA and protein expression and the production of H(2)S in rats with liver cirrhosis.
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spelling pubmed-46653412015-12-14 Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis GUO, SHI-BIN DUAN, ZHI-JUN WANG, QIU-MING ZHOU, QIN LI, QING SUN, XIAO-YU Exp Ther Med Articles The aim of the present study was to investigate the effect of endogenous carbon monoxide (CO) on the hydrogen sulfide/cystathionine-γ-lyase (H(2)S/CSE) pathway in cirrhotic rat livers. The rats were allocated at random into four groups: Sham, cirrhosis, cobalt protoporphyrin (CoPP) and zinc protoporphyrin IX (ZnPP). The expression of hepatic CSE mRNA was evaluated using a quantitative polymerase chain reaction, while CSE protein expression was determined using immunohistochemical analysis. Hematoxylin and eosin staining was performed for the histological evaluation of liver fibrosis. The levels of H(2)S, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and carboxyhemoglobin (COHb) in the arterial blood were determined, in addition to the portal vein pressure. The mRNA and protein expression levels of hepatic CSE and the serum levels of H(2)S were significantly decreased in the cirrhosis group compared with those in the sham group (P<0.05). Compared with the cirrhosis group, rats in the ZnPP group had significantly lower levels of serum ALT, AST and TBIL, arterial COHb and hepatic fibrosis, while hepatic CSE expression and the production of H(2)S were significantly increased (P<0.05). The CoPP group exhibited decreased hepatic CSE expression and H(2)S production, but aggravated hepatic function and fibrosis (P<0.05). In conclusion, the H(2)S/CSE pathway is involved in the formation of liver cirrhosis and serves a crucial function in protecting liver cells against the progression of liver fibrosis. Endogenous CO downregulates hepatic CSE mRNA and protein expression and the production of H(2)S in rats with liver cirrhosis. D.A. Spandidos 2015-12 2015-10-22 /pmc/articles/PMC4665341/ /pubmed/26668593 http://dx.doi.org/10.3892/etm.2015.2823 Text en Copyright: © Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
GUO, SHI-BIN
DUAN, ZHI-JUN
WANG, QIU-MING
ZHOU, QIN
LI, QING
SUN, XIAO-YU
Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis
title Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis
title_full Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis
title_fullStr Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis
title_full_unstemmed Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis
title_short Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis
title_sort endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665341/
https://www.ncbi.nlm.nih.gov/pubmed/26668593
http://dx.doi.org/10.3892/etm.2015.2823
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