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Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils

The main objective of this study was the screening of some selected aromatic plants very popular in Greece, with respect to their total phenolic content, antioxidant capacity, reducing activity, and oxidative stability. All plants were extracted with the conventional method, reflux with methanol. Th...

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Autores principales: Proestos, Charalampos, Lytoudi, Konstantina, Mavromelanidou, Olga Konstantina, Zoumpoulakis, Panagiotis, Sinanoglou, Vassileia J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665401/
https://www.ncbi.nlm.nih.gov/pubmed/26787619
http://dx.doi.org/10.3390/antiox2010011
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author Proestos, Charalampos
Lytoudi, Konstantina
Mavromelanidou, Olga Konstantina
Zoumpoulakis, Panagiotis
Sinanoglou, Vassileia J.
author_facet Proestos, Charalampos
Lytoudi, Konstantina
Mavromelanidou, Olga Konstantina
Zoumpoulakis, Panagiotis
Sinanoglou, Vassileia J.
author_sort Proestos, Charalampos
collection PubMed
description The main objective of this study was the screening of some selected aromatic plants very popular in Greece, with respect to their total phenolic content, antioxidant capacity, reducing activity, and oxidative stability. All plants were extracted with the conventional method, reflux with methanol. The essential oils of the plants were also analyzed for their antioxidant properties. The total phenolic content was determined by the Folin-Ciocalteu method using gallic acid as the standard, while the phenolic substances were identified and quantified by High Performance Liquid Chromatography (HPLC) coupled with a multi-wavelength ultraviolet-visible (UV-vis) detector. The antioxidant capacity of the plant extracts was measured by their ability to scavenge free radicals such as (a) DPPH (2,2-diphenyl-1-picrylhydrazyl) and, (b) ABTS (2,2′-azinobis-(3-ethylbenzothiaziline-6-sulfonate). The Folin-Ciocalteu method proved the existence of antioxidants in the aromatic plant extracts. Taking into account the results of the DPPH and ABTS methods, the free radical scavenging capacity was confirmed. Eventually, all plants exhibited low but noticeable protection levels against lipid oxidation, as determined by the Rancimat test.
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spelling pubmed-46654012016-01-14 Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils Proestos, Charalampos Lytoudi, Konstantina Mavromelanidou, Olga Konstantina Zoumpoulakis, Panagiotis Sinanoglou, Vassileia J. Antioxidants (Basel) Article The main objective of this study was the screening of some selected aromatic plants very popular in Greece, with respect to their total phenolic content, antioxidant capacity, reducing activity, and oxidative stability. All plants were extracted with the conventional method, reflux with methanol. The essential oils of the plants were also analyzed for their antioxidant properties. The total phenolic content was determined by the Folin-Ciocalteu method using gallic acid as the standard, while the phenolic substances were identified and quantified by High Performance Liquid Chromatography (HPLC) coupled with a multi-wavelength ultraviolet-visible (UV-vis) detector. The antioxidant capacity of the plant extracts was measured by their ability to scavenge free radicals such as (a) DPPH (2,2-diphenyl-1-picrylhydrazyl) and, (b) ABTS (2,2′-azinobis-(3-ethylbenzothiaziline-6-sulfonate). The Folin-Ciocalteu method proved the existence of antioxidants in the aromatic plant extracts. Taking into account the results of the DPPH and ABTS methods, the free radical scavenging capacity was confirmed. Eventually, all plants exhibited low but noticeable protection levels against lipid oxidation, as determined by the Rancimat test. MDPI 2013-01-04 /pmc/articles/PMC4665401/ /pubmed/26787619 http://dx.doi.org/10.3390/antiox2010011 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Proestos, Charalampos
Lytoudi, Konstantina
Mavromelanidou, Olga Konstantina
Zoumpoulakis, Panagiotis
Sinanoglou, Vassileia J.
Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils
title Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils
title_full Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils
title_fullStr Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils
title_full_unstemmed Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils
title_short Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils
title_sort antioxidant capacity of selected plant extracts and their essential oils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665401/
https://www.ncbi.nlm.nih.gov/pubmed/26787619
http://dx.doi.org/10.3390/antiox2010011
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