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Identification of Phenolic Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Euphorbia Tirucalli L.
Bioactive compounds extracted from natural sources can benefit human health. The aim of this work was to determine total phenolic content and antioxidant activity in extracts of Euphorbia tirucalli L. followed by identification and quantification of the phenolic compounds, as well as their antibacte...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665452/ https://www.ncbi.nlm.nih.gov/pubmed/26784670 http://dx.doi.org/10.3390/antiox3010159 |
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author | de Araújo, Keline Medeiros de Lima, Alessandro Silva, Jurandy do N. Rodrigues, Larissa L. Amorim, Adriany G. N. Quelemes, Patrick V. dos Santos, Raimunda C. Rocha, Jefferson A. de Andrades, Éryka O. Leite, José Roberto S. A. Mancini-Filho, Jorge da Trindade, Reginaldo Almeida |
author_facet | de Araújo, Keline Medeiros de Lima, Alessandro Silva, Jurandy do N. Rodrigues, Larissa L. Amorim, Adriany G. N. Quelemes, Patrick V. dos Santos, Raimunda C. Rocha, Jefferson A. de Andrades, Éryka O. Leite, José Roberto S. A. Mancini-Filho, Jorge da Trindade, Reginaldo Almeida |
author_sort | de Araújo, Keline Medeiros |
collection | PubMed |
description | Bioactive compounds extracted from natural sources can benefit human health. The aim of this work was to determine total phenolic content and antioxidant activity in extracts of Euphorbia tirucalli L. followed by identification and quantification of the phenolic compounds, as well as their antibacterial activities. Antioxidant activities were determined by DPPH and ABTS(•+) assay. Identification of phenolic compounds was performed using high-performance liquid chromatography (HPLC), and antimicrobial activities were verified by agar dilution methods and MIC values. Total phenolic content ranged from 7.73 to 30.54 mg/100 g gallic acid equivalent. Extracts from dry plants showed higher antioxidant activities than those from fresh ones. The DPPH EC(50) values were approximately 12.15 μg/mL and 16.59 μg/mL, respectively. Antioxidant activity measured by the ABTS method yielded values higher than 718.99 μM trolox/g for dry plants, while by the Rancimat(®) system yielded protection factors exceeding 1 for all extracts, comparable to synthetic BHT. Ferulic acid was the principal phenolic compound identified and quantified through HPLC-UV in all extracts. The extracts proved effective inhibitory potential for Staphylococcus epidermidis and Staphylococcus aureus. These results showed that extracts of Euphorbia tirucalli L. have excellent antioxidant capacity and moderate antimicrobial activity. These can be attributed to the high concentration of ferulic acid. |
format | Online Article Text |
id | pubmed-4665452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-46654522016-01-14 Identification of Phenolic Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Euphorbia Tirucalli L. de Araújo, Keline Medeiros de Lima, Alessandro Silva, Jurandy do N. Rodrigues, Larissa L. Amorim, Adriany G. N. Quelemes, Patrick V. dos Santos, Raimunda C. Rocha, Jefferson A. de Andrades, Éryka O. Leite, José Roberto S. A. Mancini-Filho, Jorge da Trindade, Reginaldo Almeida Antioxidants (Basel) Article Bioactive compounds extracted from natural sources can benefit human health. The aim of this work was to determine total phenolic content and antioxidant activity in extracts of Euphorbia tirucalli L. followed by identification and quantification of the phenolic compounds, as well as their antibacterial activities. Antioxidant activities were determined by DPPH and ABTS(•+) assay. Identification of phenolic compounds was performed using high-performance liquid chromatography (HPLC), and antimicrobial activities were verified by agar dilution methods and MIC values. Total phenolic content ranged from 7.73 to 30.54 mg/100 g gallic acid equivalent. Extracts from dry plants showed higher antioxidant activities than those from fresh ones. The DPPH EC(50) values were approximately 12.15 μg/mL and 16.59 μg/mL, respectively. Antioxidant activity measured by the ABTS method yielded values higher than 718.99 μM trolox/g for dry plants, while by the Rancimat(®) system yielded protection factors exceeding 1 for all extracts, comparable to synthetic BHT. Ferulic acid was the principal phenolic compound identified and quantified through HPLC-UV in all extracts. The extracts proved effective inhibitory potential for Staphylococcus epidermidis and Staphylococcus aureus. These results showed that extracts of Euphorbia tirucalli L. have excellent antioxidant capacity and moderate antimicrobial activity. These can be attributed to the high concentration of ferulic acid. MDPI 2014-03-17 /pmc/articles/PMC4665452/ /pubmed/26784670 http://dx.doi.org/10.3390/antiox3010159 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article de Araújo, Keline Medeiros de Lima, Alessandro Silva, Jurandy do N. Rodrigues, Larissa L. Amorim, Adriany G. N. Quelemes, Patrick V. dos Santos, Raimunda C. Rocha, Jefferson A. de Andrades, Éryka O. Leite, José Roberto S. A. Mancini-Filho, Jorge da Trindade, Reginaldo Almeida Identification of Phenolic Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Euphorbia Tirucalli L. |
title | Identification of Phenolic Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Euphorbia Tirucalli L. |
title_full | Identification of Phenolic Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Euphorbia Tirucalli L. |
title_fullStr | Identification of Phenolic Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Euphorbia Tirucalli L. |
title_full_unstemmed | Identification of Phenolic Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Euphorbia Tirucalli L. |
title_short | Identification of Phenolic Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Euphorbia Tirucalli L. |
title_sort | identification of phenolic compounds and evaluation of antioxidant and antimicrobial properties of euphorbia tirucalli l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665452/ https://www.ncbi.nlm.nih.gov/pubmed/26784670 http://dx.doi.org/10.3390/antiox3010159 |
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