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Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves

In the quest for new medicines, the methanolic, hexanic, and aqueous extracts of Adiantum caudatum leaves, obtained by Soxhlet extraction, were analyzed for phenolic and flavonoid contents, and antioxidant and antimicrobial potential. TPCs (total phenolic content) of the methanolic, aqueous and hexa...

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Autores principales: Ahmed, Dildar, Khan, Muhammad Mehboob, Saeed, Ramsha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665467/
https://www.ncbi.nlm.nih.gov/pubmed/26783712
http://dx.doi.org/10.3390/antiox4020394
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author Ahmed, Dildar
Khan, Muhammad Mehboob
Saeed, Ramsha
author_facet Ahmed, Dildar
Khan, Muhammad Mehboob
Saeed, Ramsha
author_sort Ahmed, Dildar
collection PubMed
description In the quest for new medicines, the methanolic, hexanic, and aqueous extracts of Adiantum caudatum leaves, obtained by Soxhlet extraction, were analyzed for phenolic and flavonoid contents, and antioxidant and antimicrobial potential. TPCs (total phenolic content) of the methanolic, aqueous and hexanic extracts were 27.7, 21.1, and 16.7 μg of gallic acid equivalents per mL, respectively, while TFCs (total flavonoid content) were 13.2, 11.6, and 10.0 μg of rutin equivalents per mL, respectively. Antioxidant activities of the extracts in reducing power, FRAP (ferric reducing antioxidant power), phosphomolybdate and ABTS assays follow the same order of methanolic ˃ aqueous ˃ hexanic. In the DPPH assay, however, the aqueous extract exhibited a slightly higher antioxidant activity than the methanolic one. Methanol is therefore a better solvent to extract most of the antioxidant components from A. caudatum leaves. In lipid peroxidation inhibitory assay, the extracts showed almost similar behavior and their activity decreased gradually with time. The aqueous extract was the strongest inhibitor after two days, but the hexanic became the most potent after about three days. The antibacterial potential of the extracts was determined against Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. Towards all the microbes, the aqueous extract was the most potent and the hexanic the least. P. aeruginosa was the most susceptible strain, while the aqueous and methanolic extracts exhibited a slightly higher efficacy against this pathogen than the drug amoxicillin. In conclusion, A. caudatum can potentially provide a remedy against disorders caused by oxidative stress and infections.
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spelling pubmed-46654672016-01-14 Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves Ahmed, Dildar Khan, Muhammad Mehboob Saeed, Ramsha Antioxidants (Basel) Article In the quest for new medicines, the methanolic, hexanic, and aqueous extracts of Adiantum caudatum leaves, obtained by Soxhlet extraction, were analyzed for phenolic and flavonoid contents, and antioxidant and antimicrobial potential. TPCs (total phenolic content) of the methanolic, aqueous and hexanic extracts were 27.7, 21.1, and 16.7 μg of gallic acid equivalents per mL, respectively, while TFCs (total flavonoid content) were 13.2, 11.6, and 10.0 μg of rutin equivalents per mL, respectively. Antioxidant activities of the extracts in reducing power, FRAP (ferric reducing antioxidant power), phosphomolybdate and ABTS assays follow the same order of methanolic ˃ aqueous ˃ hexanic. In the DPPH assay, however, the aqueous extract exhibited a slightly higher antioxidant activity than the methanolic one. Methanol is therefore a better solvent to extract most of the antioxidant components from A. caudatum leaves. In lipid peroxidation inhibitory assay, the extracts showed almost similar behavior and their activity decreased gradually with time. The aqueous extract was the strongest inhibitor after two days, but the hexanic became the most potent after about three days. The antibacterial potential of the extracts was determined against Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. Towards all the microbes, the aqueous extract was the most potent and the hexanic the least. P. aeruginosa was the most susceptible strain, while the aqueous and methanolic extracts exhibited a slightly higher efficacy against this pathogen than the drug amoxicillin. In conclusion, A. caudatum can potentially provide a remedy against disorders caused by oxidative stress and infections. MDPI 2015-06-04 /pmc/articles/PMC4665467/ /pubmed/26783712 http://dx.doi.org/10.3390/antiox4020394 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahmed, Dildar
Khan, Muhammad Mehboob
Saeed, Ramsha
Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves
title Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves
title_full Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves
title_fullStr Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves
title_full_unstemmed Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves
title_short Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves
title_sort comparative analysis of phenolics, flavonoids, and antioxidant and antibacterial potential of methanolic, hexanic and aqueous extracts from adiantum caudatum leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665467/
https://www.ncbi.nlm.nih.gov/pubmed/26783712
http://dx.doi.org/10.3390/antiox4020394
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