Cargando…

An improved SYBR Green-1-based fluorescence method for the routine monitoring of Plasmodium falciparum resistance to anti-malarial drugs

BACKGROUND: The recently introduced SYBR Green1 (SG) assay for testing parasites susceptibility to anti-malarial drugs needs further improvement. This has been necessitated by various setbacks, the major one being the low fluorescence intensity associated with it use. This shortcoming diminishes the...

Descripción completa

Detalles Bibliográficos
Autores principales:	Dery, Victor, Duah, Nancy O., Ayanful-Torgby, Ruth, Matrevi, Sena A., Anto, Francis, Quashie, Neils B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665889/ https://www.ncbi.nlm.nih.gov/pubmed/26625907 http://dx.doi.org/10.1186/s12936-015-1011-x

Ejemplares similares

A SYBR Green 1-based in vitro test of susceptibility of Ghanaian Plasmodium falciparum clinical isolates to a panel of anti-malarial drugs
por: Quashie, Neils B, et al.
Publicado: (2013)

Genetic diversity of Plasmodium falciparum isolates from uncomplicated malaria cases in Ghana over a decade
por: Duah, Nancy O., et al.
Publicado: (2016)

Plasmodium falciparum genetic factors rather than host factors are likely to drive resistance to ACT in Ghana
por: Hodoameda, Peter, et al.
Publicado: (2020)

Seasonal variations in Plasmodium falciparum parasite prevalence assessed by varying diagnostic tests in asymptomatic children in southern Ghana
por: Ayanful-Torgby, Ruth, et al.
Publicado: (2018)

Amplification of GTP-cyclohydrolase 1 gene in Plasmodium falciparum isolates with the quadruple mutant of dihydrofolate reductase and dihydropteroate synthase genes in Ghana
por: Osei, Musah, et al.
Publicado: (2018)

An improved and highly sensitive microfluorimetric method for assessing susceptibility of Plasmodium falciparum to antimalarial drugs in vitro
por: Quashie, Neils B, et al.
Publicado: (2006)

Novel pfk13 polymorphisms in Plasmodium falciparum population in Ghana
por: Matrevi, Sena Adzoa, et al.
Publicado: (2022)

Increased pfmdr1 gene copy number and the decline in pfcrt and pfmdr1 resistance alleles in Ghanaian Plasmodium falciparum isolates after the change of anti-malarial drug treatment policy
por: Duah, Nancy O, et al.
Publicado: (2013)

Prevalence of Plasmodium falciparum delayed clearance associated polymorphisms in adaptor protein complex 2 mu subunit (pfap2mu) and ubiquitin specific protease 1 (pfubp1) genes in Ghanaian isolates
por: Adams, Tryphena, et al.
Publicado: (2018)

Transcriptome-module phenotype association study implicates extracellular vesicles biogenesis in Plasmodium falciparum artemisinin resistance
por: Tandoh, Kwesi Z., et al.
Publicado: (2022)

Real-time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
por: Ferreira, Isabel D, et al.
Publicado: (2006)

Plasmodium falciparum Malaria Parasites in Ghana Show Signatures of Balancing Selection at Artemisinin Resistance Predisposing Background Genes
por: Tandoh, Kwesi Z, et al.
Publicado: (2021)

Therapeutic efficacy of artesunate–amodiaquine and artemether–lumefantrine combinations for uncomplicated malaria in 10 sentinel sites across Ghana: 2015–2017
por: Abuaku, Benjamin, et al.
Publicado: (2019)

Seasonal variations in Plasmodium falciparum genetic diversity and multiplicity of infection in asymptomatic children living in southern Ghana
por: Adjah, Joshua, et al.
Publicado: (2018)

Plasmodium falciparum genotype and gametocyte prevalence in children with uncomplicated malaria in coastal Ghana
por: Ayanful-Torgby, Ruth, et al.
Publicado: (2016)

Assessment of artemisinin tolerance in Plasmodium falciparum clinical isolates in children with uncomplicated malaria in Ghana
por: Ahorhorlu, Samuel Yao, et al.
Publicado: (2023)

Optimization of a multi-well colorimetric assay to determine haem species in Plasmodium falciparum in the presence of anti-malarials
por: Combrinck, Jill M., et al.
Publicado: (2015)

Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
por: Smith-Aguasca, Rebecca, et al.
Publicado: (2019)

Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays
por: Wilson, Danny W, et al.
Publicado: (2010)

Prevalence of G6PD deficiency and Plasmodium falciparum parasites in asymptomatic school children living in southern Ghana
por: Amoah, Linda Eva, et al.
Publicado: (2016)

Estimation of parasite age and synchrony status in Plasmodium falciparum infections
por: Ciuffreda, Laura, et al.
Publicado: (2020)

A new double-antibody sandwich ELISA targeting Plasmodium falciparum aldolase to evaluate anti-malarial drug sensitivity
por: Tritten, Lucienne, et al.
Publicado: (2009)

Recent Molecular Assessment of Plasmodium vivax and Plasmodium falciparum Asymptomatic Infections in Botswana
por: Motshoge, Thato, et al.
Publicado: (2021)

Assessment of LED fluorescence microscopy for the diagnosis of Plasmodium falciparum infections in Gabon
por: Lenz, Dominic, et al.
Publicado: (2011)

Drying anti-malarial drugs in vitro tests to outsource SYBR green assays
por: Traore, Karim, et al.
Publicado: (2015)

Diversity and immune responses against Plasmodium falciparum gametocytes in non-febrile school children living in Southern Ghana
por: Amoah, Linda E., et al.
Publicado: (2019)

Therapeutic efficacy of dihydroartemisinin-piperaquine combination for the treatment of uncomplicated malaria in Ghana
por: Abuaku, Benjamin, et al.
Publicado: (2023)

Assessing the Roles of Molecular Markers of Antimalarial Drug Resistance and the Host Pharmacogenetics in Drug-Resistant Malaria
por: Hodoameda, Peter, et al.
Publicado: (2022)

Persistent Plasmodium falciparum infections enhance transmission-reducing immunity development
por: Ayanful-Torgby, Ruth, et al.
Publicado: (2021)

Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A
por: Jang, Jin Woo, et al.
Publicado: (2014)

A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition
por: Scipioni, Alexandra, et al.
Publicado: (2008)

Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma
por: Tyagi, Anuj Kumar, et al.
Publicado: (2013)

Improved negative selection protocol for Plasmodium berghei in the rodent malarial model
por: Orr, Rachael Y, et al.
Publicado: (2012)

A protein-centric approach for the identification of folate enzymes from the malarial parasite, Plasmodium falciparum, using OFFGEL™ solution-based isoelectric focussing and mass spectrometry
por: O'Cualain, Ronan DM, et al.
Publicado: (2010)

Limit of blank and limit of detection of Plasmodium falciparum thick blood smear microscopy in a routine setting in Central Africa
por: Joanny, Fanny, et al.
Publicado: (2014)

Fluorescence detection of malachite green in fish based on aptamer and SYBR Green I
por: Wang, Jia, et al.
Publicado: (2022)

In vivo transcriptional profiling of Plasmodium falciparum
por: Daily, Johanna P, et al.
Publicado: (2004)

Laser-induced inactivation of Plasmodium falciparum
por: LeBlanc, Danielle, et al.
Publicado: (2012)

New synchronization method for Plasmodium falciparum
por: Ranford-Cartwright, Lisa C, et al.
Publicado: (2010)

Comparison of Plasmodium falciparum transfection methods
por: Skinner-Adams, Tina S, et al.
Publicado: (2003)

Cannot write session to /tmp/vufind_sessions/sess_8dbbrrg8bb4ld6rllukdj7e1uq