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A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes
PIWI interacting RNAs (piRNAs) are highly expressed in germline cells and are involved in maintaining genome integrity by silencing transposons. These are also involved in DNA/histone methylation and gene expression regulation in somatic cells of invertebrates. The functions of piRNAs in somatic cel...
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666397/ https://www.ncbi.nlm.nih.gov/pubmed/26405199 http://dx.doi.org/10.1093/nar/gkv954 |
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| author | Zhong, Fudi Zhou, Nan Wu, Kang Guo, Yubiao Tan, Weiping Zhang, Hong Zhang, Xue Geng, Guannan Pan, Ting Luo, Haihua Zhang, Yijun Xu, Zhibin Liu, Jun Liu, Bingfeng Gao, Wenchao Liu, Chao Ren, Liangliang Li, Jun Zhou, Jie Zhang, Hui |
| author_facet | Zhong, Fudi Zhou, Nan Wu, Kang Guo, Yubiao Tan, Weiping Zhang, Hong Zhang, Xue Geng, Guannan Pan, Ting Luo, Haihua Zhang, Yijun Xu, Zhibin Liu, Jun Liu, Bingfeng Gao, Wenchao Liu, Chao Ren, Liangliang Li, Jun Zhou, Jie Zhang, Hui |
| author_sort | Zhong, Fudi |
| collection | PubMed |
| description | PIWI interacting RNAs (piRNAs) are highly expressed in germline cells and are involved in maintaining genome integrity by silencing transposons. These are also involved in DNA/histone methylation and gene expression regulation in somatic cells of invertebrates. The functions of piRNAs in somatic cells of vertebrates, however, remain elusive. We found that snoRNA-derived and C (C′)/D′ (D)-box conserved piRNAs are abundant in human CD4 primary T-lymphocytes. piRNA (piR30840) significantly downregulated interleukin-4 (IL-4) via sequence complementarity binding to pre-mRNA intron, which subsequently inhibited the development of Th2 T-lymphocytes. Piwil4 and Ago4 are associated with this piRNA, and this complex further interacts with Trf4-Air2-Mtr4 Polyadenylation (TRAMP) complex, which leads to the decay of targeted pre-mRNA through nuclear exosomes. Taken together, we demonstrate a novel piRNA mechanism in regulating gene expression in highly differentiated somatic cells and a possible novel target for allergy therapeutics. |
| format | Online Article Text |
| id | pubmed-4666397 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| publisher | Oxford University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-46663972015-12-02 A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes Zhong, Fudi Zhou, Nan Wu, Kang Guo, Yubiao Tan, Weiping Zhang, Hong Zhang, Xue Geng, Guannan Pan, Ting Luo, Haihua Zhang, Yijun Xu, Zhibin Liu, Jun Liu, Bingfeng Gao, Wenchao Liu, Chao Ren, Liangliang Li, Jun Zhou, Jie Zhang, Hui Nucleic Acids Res RNA PIWI interacting RNAs (piRNAs) are highly expressed in germline cells and are involved in maintaining genome integrity by silencing transposons. These are also involved in DNA/histone methylation and gene expression regulation in somatic cells of invertebrates. The functions of piRNAs in somatic cells of vertebrates, however, remain elusive. We found that snoRNA-derived and C (C′)/D′ (D)-box conserved piRNAs are abundant in human CD4 primary T-lymphocytes. piRNA (piR30840) significantly downregulated interleukin-4 (IL-4) via sequence complementarity binding to pre-mRNA intron, which subsequently inhibited the development of Th2 T-lymphocytes. Piwil4 and Ago4 are associated with this piRNA, and this complex further interacts with Trf4-Air2-Mtr4 Polyadenylation (TRAMP) complex, which leads to the decay of targeted pre-mRNA through nuclear exosomes. Taken together, we demonstrate a novel piRNA mechanism in regulating gene expression in highly differentiated somatic cells and a possible novel target for allergy therapeutics. Oxford University Press 2015-12-02 2015-09-23 /pmc/articles/PMC4666397/ /pubmed/26405199 http://dx.doi.org/10.1093/nar/gkv954 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| spellingShingle | RNA Zhong, Fudi Zhou, Nan Wu, Kang Guo, Yubiao Tan, Weiping Zhang, Hong Zhang, Xue Geng, Guannan Pan, Ting Luo, Haihua Zhang, Yijun Xu, Zhibin Liu, Jun Liu, Bingfeng Gao, Wenchao Liu, Chao Ren, Liangliang Li, Jun Zhou, Jie Zhang, Hui A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes |
| title | A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes |
| title_full | A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes |
| title_fullStr | A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes |
| title_full_unstemmed | A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes |
| title_short | A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes |
| title_sort | snorna-derived pirna interacts with human interleukin-4 pre-mrna and induces its decay in nuclear exosomes |
| topic | RNA |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666397/ https://www.ncbi.nlm.nih.gov/pubmed/26405199 http://dx.doi.org/10.1093/nar/gkv954 |
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