Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy

Current measurements of the biomechanical properties of cells require physical contact with cells or lack sub-cellular resolution. Here, we developed a label-free optical microscopy technique based on Brillouin light scattering capable of measuring intracellular longitudinal modulus with optical res...

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Detalles Bibliográficos
Autores principales: Scarcelli, Giuliano, Polacheck, William J., Nia, Hadi T., Patel, Kripa, Grodzinsky, Alan J., Kamm, Roger D., Yun, Seok Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666809/
https://www.ncbi.nlm.nih.gov/pubmed/26436482
http://dx.doi.org/10.1038/nmeth.3616
Descripción
Sumario:Current measurements of the biomechanical properties of cells require physical contact with cells or lack sub-cellular resolution. Here, we developed a label-free optical microscopy technique based on Brillouin light scattering capable of measuring intracellular longitudinal modulus with optical resolution. We obtained 3D Brillouin maps of cells in 2D and 3D microenvironments, which reveal mechanical changes due to cytoskeletal modulation and cell volume regulation.

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