Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy

Current measurements of the biomechanical properties of cells require physical contact with cells or lack sub-cellular resolution. Here, we developed a label-free optical microscopy technique based on Brillouin light scattering capable of measuring intracellular longitudinal modulus with optical res...

Descripción completa

Detalles Bibliográficos
Autores principales: Scarcelli, Giuliano, Polacheck, William J., Nia, Hadi T., Patel, Kripa, Grodzinsky, Alan J., Kamm, Roger D., Yun, Seok Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666809/
https://www.ncbi.nlm.nih.gov/pubmed/26436482
http://dx.doi.org/10.1038/nmeth.3616
_version_ 1782403744182304768
author Scarcelli, Giuliano
Polacheck, William J.
Nia, Hadi T.
Patel, Kripa
Grodzinsky, Alan J.
Kamm, Roger D.
Yun, Seok Hyun
author_facet Scarcelli, Giuliano
Polacheck, William J.
Nia, Hadi T.
Patel, Kripa
Grodzinsky, Alan J.
Kamm, Roger D.
Yun, Seok Hyun
author_sort Scarcelli, Giuliano
collection PubMed
description Current measurements of the biomechanical properties of cells require physical contact with cells or lack sub-cellular resolution. Here, we developed a label-free optical microscopy technique based on Brillouin light scattering capable of measuring intracellular longitudinal modulus with optical resolution. We obtained 3D Brillouin maps of cells in 2D and 3D microenvironments, which reveal mechanical changes due to cytoskeletal modulation and cell volume regulation.
format Online
Article
Text
id pubmed-4666809
institution National Center for Biotechnology Information
language English
publishDate 2015
record_format MEDLINE/PubMed
spelling pubmed-46668092016-05-18 Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy Scarcelli, Giuliano Polacheck, William J. Nia, Hadi T. Patel, Kripa Grodzinsky, Alan J. Kamm, Roger D. Yun, Seok Hyun Nat Methods Article Current measurements of the biomechanical properties of cells require physical contact with cells or lack sub-cellular resolution. Here, we developed a label-free optical microscopy technique based on Brillouin light scattering capable of measuring intracellular longitudinal modulus with optical resolution. We obtained 3D Brillouin maps of cells in 2D and 3D microenvironments, which reveal mechanical changes due to cytoskeletal modulation and cell volume regulation. 2015-10-05 2015-12 /pmc/articles/PMC4666809/ /pubmed/26436482 http://dx.doi.org/10.1038/nmeth.3616 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Scarcelli, Giuliano
Polacheck, William J.
Nia, Hadi T.
Patel, Kripa
Grodzinsky, Alan J.
Kamm, Roger D.
Yun, Seok Hyun
Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy
title Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy
title_full Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy
title_fullStr Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy
title_full_unstemmed Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy
title_short Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy
title_sort noncontact three-dimensional mapping of intracellular hydro-mechanical properties by brillouin microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666809/
https://www.ncbi.nlm.nih.gov/pubmed/26436482
http://dx.doi.org/10.1038/nmeth.3616
work_keys_str_mv AT scarcelligiuliano noncontactthreedimensionalmappingofintracellularhydromechanicalpropertiesbybrillouinmicroscopy
AT polacheckwilliamj noncontactthreedimensionalmappingofintracellularhydromechanicalpropertiesbybrillouinmicroscopy
AT niahadit noncontactthreedimensionalmappingofintracellularhydromechanicalpropertiesbybrillouinmicroscopy
AT patelkripa noncontactthreedimensionalmappingofintracellularhydromechanicalpropertiesbybrillouinmicroscopy
AT grodzinskyalanj noncontactthreedimensionalmappingofintracellularhydromechanicalpropertiesbybrillouinmicroscopy
AT kammrogerd noncontactthreedimensionalmappingofintracellularhydromechanicalpropertiesbybrillouinmicroscopy
AT yunseokhyun noncontactthreedimensionalmappingofintracellularhydromechanicalpropertiesbybrillouinmicroscopy

Ejemplares similares