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Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins

The targeting signals and mechanisms of soluble peroxisomal proteins are well understood, whereas less is known about the signals and targeting routes of peroxisomal membrane proteins (PMP). Pex15 and PEX26, tail-anchored proteins in yeast and mammals, respectively, exert a similar cellular function...

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Autores principales: Buentzel, Judith, Vilardi, Fabio, Lotz-Havla, Amelie, Gärtner, Jutta, Thoms, Sven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667187/
https://www.ncbi.nlm.nih.gov/pubmed/26627908
http://dx.doi.org/10.1038/srep17420
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author Buentzel, Judith
Vilardi, Fabio
Lotz-Havla, Amelie
Gärtner, Jutta
Thoms, Sven
author_facet Buentzel, Judith
Vilardi, Fabio
Lotz-Havla, Amelie
Gärtner, Jutta
Thoms, Sven
author_sort Buentzel, Judith
collection PubMed
description The targeting signals and mechanisms of soluble peroxisomal proteins are well understood, whereas less is known about the signals and targeting routes of peroxisomal membrane proteins (PMP). Pex15 and PEX26, tail-anchored proteins in yeast and mammals, respectively, exert a similar cellular function in the recruitment of AAA peroxins at the peroxisomal membrane. But despite their common role, Pex15 and PEX26 are neither homologs nor they are known to follow similar targeting principles. Here we show that Pex15 targets to peroxisomes in mammalian cells, and PEX26 reaches peroxisomes when expressed in yeast cells. In both proteins C-terminal targeting information is sufficient for correct sorting to the peroxisomal membrane. In yeast, PEX26 follows the pathway that also ensures correct targeting of Pex15: PEX26 enters the endoplasmic reticulum (ER) in a GET-dependent and Pex19-independent manner. Like in yeast, PEX26 enters the ER in mammalian cells, however, independently of GET/TRC40. These data show that conserved targeting information is employed in yeast and higher eukaryotes during the biogenesis of peroxisomal tail-anchored proteins.
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spelling pubmed-46671872015-12-08 Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins Buentzel, Judith Vilardi, Fabio Lotz-Havla, Amelie Gärtner, Jutta Thoms, Sven Sci Rep Article The targeting signals and mechanisms of soluble peroxisomal proteins are well understood, whereas less is known about the signals and targeting routes of peroxisomal membrane proteins (PMP). Pex15 and PEX26, tail-anchored proteins in yeast and mammals, respectively, exert a similar cellular function in the recruitment of AAA peroxins at the peroxisomal membrane. But despite their common role, Pex15 and PEX26 are neither homologs nor they are known to follow similar targeting principles. Here we show that Pex15 targets to peroxisomes in mammalian cells, and PEX26 reaches peroxisomes when expressed in yeast cells. In both proteins C-terminal targeting information is sufficient for correct sorting to the peroxisomal membrane. In yeast, PEX26 follows the pathway that also ensures correct targeting of Pex15: PEX26 enters the endoplasmic reticulum (ER) in a GET-dependent and Pex19-independent manner. Like in yeast, PEX26 enters the ER in mammalian cells, however, independently of GET/TRC40. These data show that conserved targeting information is employed in yeast and higher eukaryotes during the biogenesis of peroxisomal tail-anchored proteins. Nature Publishing Group 2015-12-02 /pmc/articles/PMC4667187/ /pubmed/26627908 http://dx.doi.org/10.1038/srep17420 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Buentzel, Judith
Vilardi, Fabio
Lotz-Havla, Amelie
Gärtner, Jutta
Thoms, Sven
Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins
title Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins
title_full Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins
title_fullStr Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins
title_full_unstemmed Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins
title_short Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins
title_sort conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667187/
https://www.ncbi.nlm.nih.gov/pubmed/26627908
http://dx.doi.org/10.1038/srep17420
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