Native sulfur/chlorine SAD phasing for serial femtosecond crystallography

Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous si...

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Autores principales: Nakane, Takanori, Song, Changyong, Suzuki, Mamoru, Nango, Eriko, Kobayashi, Jun, Masuda, Tetsuya, Inoue, Shigeyuki, Mizohata, Eiichi, Nakatsu, Toru, Tanaka, Tomoyuki, Tanaka, Rie, Shimamura, Tatsuro, Tono, Kensuke, Joti, Yasumasa, Kameshima, Takashi, Hatsui, Takaki, Yabashi, Makina, Nureki, Osamu, Iwata, So, Sugahara, Michihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667287/
https://www.ncbi.nlm.nih.gov/pubmed/26627659
http://dx.doi.org/10.1107/S139900471501857X
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author Nakane, Takanori
Song, Changyong
Suzuki, Mamoru
Nango, Eriko
Kobayashi, Jun
Masuda, Tetsuya
Inoue, Shigeyuki
Mizohata, Eiichi
Nakatsu, Toru
Tanaka, Tomoyuki
Tanaka, Rie
Shimamura, Tatsuro
Tono, Kensuke
Joti, Yasumasa
Kameshima, Takashi
Hatsui, Takaki
Yabashi, Makina
Nureki, Osamu
Iwata, So
Sugahara, Michihiro
author_facet Nakane, Takanori
Song, Changyong
Suzuki, Mamoru
Nango, Eriko
Kobayashi, Jun
Masuda, Tetsuya
Inoue, Shigeyuki
Mizohata, Eiichi
Nakatsu, Toru
Tanaka, Tomoyuki
Tanaka, Rie
Shimamura, Tatsuro
Tono, Kensuke
Joti, Yasumasa
Kameshima, Takashi
Hatsui, Takaki
Yabashi, Makina
Nureki, Osamu
Iwata, So
Sugahara, Michihiro
author_sort Nakane, Takanori
collection PubMed
description Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.
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spelling pubmed-46672872015-12-10 Native sulfur/chlorine SAD phasing for serial femtosecond crystallography Nakane, Takanori Song, Changyong Suzuki, Mamoru Nango, Eriko Kobayashi, Jun Masuda, Tetsuya Inoue, Shigeyuki Mizohata, Eiichi Nakatsu, Toru Tanaka, Tomoyuki Tanaka, Rie Shimamura, Tatsuro Tono, Kensuke Joti, Yasumasa Kameshima, Takashi Hatsui, Takaki Yabashi, Makina Nureki, Osamu Iwata, So Sugahara, Michihiro Acta Crystallogr D Biol Crystallogr Research Papers Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures. International Union of Crystallography 2015-11-27 /pmc/articles/PMC4667287/ /pubmed/26627659 http://dx.doi.org/10.1107/S139900471501857X Text en © Nakane et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Nakane, Takanori
Song, Changyong
Suzuki, Mamoru
Nango, Eriko
Kobayashi, Jun
Masuda, Tetsuya
Inoue, Shigeyuki
Mizohata, Eiichi
Nakatsu, Toru
Tanaka, Tomoyuki
Tanaka, Rie
Shimamura, Tatsuro
Tono, Kensuke
Joti, Yasumasa
Kameshima, Takashi
Hatsui, Takaki
Yabashi, Makina
Nureki, Osamu
Iwata, So
Sugahara, Michihiro
Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
title Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
title_full Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
title_fullStr Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
title_full_unstemmed Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
title_short Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
title_sort native sulfur/chlorine sad phasing for serial femtosecond crystallography
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667287/
https://www.ncbi.nlm.nih.gov/pubmed/26627659
http://dx.doi.org/10.1107/S139900471501857X
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