Cargando…

High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos

Assisted reproductive technologies (ARTs) represent the best chance for infertile couples to conceive, although increased risks for morbidities exist, including imprinting disorders. This increased risk could arise from ARTs disrupting genomic imprints during gametogenesis or preimplantation. The fe...

Descripción completa

Detalles Bibliográficos
Autores principales: White, Carlee R., Denomme, Michelle M., Tekpetey, Francis R., Feyles, Valter, Power, Stephen G. A., Mann, Mellissa R. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667293/
https://www.ncbi.nlm.nih.gov/pubmed/26626153
http://dx.doi.org/10.1038/srep17311
_version_ 1782403819301240832
author White, Carlee R.
Denomme, Michelle M.
Tekpetey, Francis R.
Feyles, Valter
Power, Stephen G. A.
Mann, Mellissa R. W.
author_facet White, Carlee R.
Denomme, Michelle M.
Tekpetey, Francis R.
Feyles, Valter
Power, Stephen G. A.
Mann, Mellissa R. W.
author_sort White, Carlee R.
collection PubMed
description Assisted reproductive technologies (ARTs) represent the best chance for infertile couples to conceive, although increased risks for morbidities exist, including imprinting disorders. This increased risk could arise from ARTs disrupting genomic imprints during gametogenesis or preimplantation. The few studies examining ART effects on genomic imprinting primarily assessed poor quality human embryos. Here, we examined day 3 and blastocyst stage, good to high quality, donated human embryos for imprinted SNRPN, KCNQ1OT1 and H19 methylation. Seventy-six percent day 3 embryos and 50% blastocysts exhibited perturbed imprinted methylation, demonstrating that extended culture did not pose greater risk for imprinting errors than short culture. Comparison of embryos with normal and abnormal methylation didn’t reveal any confounding factors. Notably, two embryos from male factor infertility patients using donor sperm harboured aberrant methylation, suggesting errors in these embryos cannot be explained by infertility alone. Overall, these results indicate that ART human preimplantation embryos possess a high frequency of imprinted methylation errors.
format Online
Article
Text
id pubmed-4667293
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-46672932015-12-08 High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos White, Carlee R. Denomme, Michelle M. Tekpetey, Francis R. Feyles, Valter Power, Stephen G. A. Mann, Mellissa R. W. Sci Rep Article Assisted reproductive technologies (ARTs) represent the best chance for infertile couples to conceive, although increased risks for morbidities exist, including imprinting disorders. This increased risk could arise from ARTs disrupting genomic imprints during gametogenesis or preimplantation. The few studies examining ART effects on genomic imprinting primarily assessed poor quality human embryos. Here, we examined day 3 and blastocyst stage, good to high quality, donated human embryos for imprinted SNRPN, KCNQ1OT1 and H19 methylation. Seventy-six percent day 3 embryos and 50% blastocysts exhibited perturbed imprinted methylation, demonstrating that extended culture did not pose greater risk for imprinting errors than short culture. Comparison of embryos with normal and abnormal methylation didn’t reveal any confounding factors. Notably, two embryos from male factor infertility patients using donor sperm harboured aberrant methylation, suggesting errors in these embryos cannot be explained by infertility alone. Overall, these results indicate that ART human preimplantation embryos possess a high frequency of imprinted methylation errors. Nature Publishing Group 2015-12-02 /pmc/articles/PMC4667293/ /pubmed/26626153 http://dx.doi.org/10.1038/srep17311 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
White, Carlee R.
Denomme, Michelle M.
Tekpetey, Francis R.
Feyles, Valter
Power, Stephen G. A.
Mann, Mellissa R. W.
High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos
title High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos
title_full High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos
title_fullStr High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos
title_full_unstemmed High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos
title_short High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos
title_sort high frequency of imprinted methylation errors in human preimplantation embryos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667293/
https://www.ncbi.nlm.nih.gov/pubmed/26626153
http://dx.doi.org/10.1038/srep17311
work_keys_str_mv AT whitecarleer highfrequencyofimprintedmethylationerrorsinhumanpreimplantationembryos
AT denommemichellem highfrequencyofimprintedmethylationerrorsinhumanpreimplantationembryos
AT tekpeteyfrancisr highfrequencyofimprintedmethylationerrorsinhumanpreimplantationembryos
AT feylesvalter highfrequencyofimprintedmethylationerrorsinhumanpreimplantationembryos
AT powerstephenga highfrequencyofimprintedmethylationerrorsinhumanpreimplantationembryos
AT mannmellissarw highfrequencyofimprintedmethylationerrorsinhumanpreimplantationembryos