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Gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach
BACKGROUND: Intrauterine growth restriction (IUGR) may program metabolic alterations affecting physiological functions and lead to diseases in later life. The adipose tissue is an important organ influencing energy homeostasis. The present study was aimed at exploring the consequences of IUGR on the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667418/ https://www.ncbi.nlm.nih.gov/pubmed/26633942 http://dx.doi.org/10.1186/s12953-015-0088-z |
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author | de Souza, Adriana Pereira Pedroso, Amanda Paula Watanabe, Regina Lúcia Harumi Dornellas, Ana Paula Segantine Boldarine, Valter Tadeu Laure, Helen Julie do Nascimento, Claudia Maria Oller Oyama, Lila Missae Rosa, José Cesar Ribeiro, Eliane Beraldi |
author_facet | de Souza, Adriana Pereira Pedroso, Amanda Paula Watanabe, Regina Lúcia Harumi Dornellas, Ana Paula Segantine Boldarine, Valter Tadeu Laure, Helen Julie do Nascimento, Claudia Maria Oller Oyama, Lila Missae Rosa, José Cesar Ribeiro, Eliane Beraldi |
author_sort | de Souza, Adriana Pereira |
collection | PubMed |
description | BACKGROUND: Intrauterine growth restriction (IUGR) may program metabolic alterations affecting physiological functions and lead to diseases in later life. The adipose tissue is an important organ influencing energy homeostasis. The present study was aimed at exploring the consequences of IUGR on the retroperitoneal adipose tissue of adult male and female rats, using a proteomic approach. METHODS AND RESULTS: Pregnant Wistar rats were fed with balanced chow, either ad libitum (control group) or restricted to 50 % of control intake (restricted group) during the whole gestation. The offspring were weaned to ad libitum chow and studied at 4 months of age. Retroperitoneal fat was analyzed by two-dimensional gel electrophoresis followed by mass spectrometry. Both male and female restricted groups had low body weight at birth and at weaning but normal body weight at adulthood. The restricted males had normal fat pads weight and serum glucose levels, with a trend to hyperinsulinemia. The restricted females had increased fat pads weight with normal glucose and insulin levels. The restricted males showed up-regulated levels of proteasome subunit α type 3, branched-chain-amino-acid aminotransferase, elongation 1- alpha 1, fatty acid synthase levels, cytosolic malate dehydrogenase and ATP synthase subunit alpha. These alterations point to increased proteolysis and lipogenesis rates and favoring of ATP generation. The restricted females showed down-regulated levels of L-lactate dehydrogenase perilipin-1, mitochondrial branched-chain alpha-keto acid dehydrogenase E1, and transketolase. These findings suggest impairment of glycemic control, stimulation of lipolysis and inhibition of proteolysis, pentose phosphate pathway and lipogenesis rates. In both genders, several proteins involved in oxidative stress and inflammation were affected, in a pattern compatible with impairment of these responses. CONCLUSIONS: The proteomic analysis of adipose tissue showed that, although IUGR affected pathways of substrate and energy metabolism in both males and females, important gender differences were evident. While IUGR males displayed alterations pointing to a predisposition to later development of obesity, the alterations observed in IUGR females pointed to a metabolic status of established obesity, in agreement with their increased fat pads mass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12953-015-0088-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4667418 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46674182015-12-03 Gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach de Souza, Adriana Pereira Pedroso, Amanda Paula Watanabe, Regina Lúcia Harumi Dornellas, Ana Paula Segantine Boldarine, Valter Tadeu Laure, Helen Julie do Nascimento, Claudia Maria Oller Oyama, Lila Missae Rosa, José Cesar Ribeiro, Eliane Beraldi Proteome Sci Research BACKGROUND: Intrauterine growth restriction (IUGR) may program metabolic alterations affecting physiological functions and lead to diseases in later life. The adipose tissue is an important organ influencing energy homeostasis. The present study was aimed at exploring the consequences of IUGR on the retroperitoneal adipose tissue of adult male and female rats, using a proteomic approach. METHODS AND RESULTS: Pregnant Wistar rats were fed with balanced chow, either ad libitum (control group) or restricted to 50 % of control intake (restricted group) during the whole gestation. The offspring were weaned to ad libitum chow and studied at 4 months of age. Retroperitoneal fat was analyzed by two-dimensional gel electrophoresis followed by mass spectrometry. Both male and female restricted groups had low body weight at birth and at weaning but normal body weight at adulthood. The restricted males had normal fat pads weight and serum glucose levels, with a trend to hyperinsulinemia. The restricted females had increased fat pads weight with normal glucose and insulin levels. The restricted males showed up-regulated levels of proteasome subunit α type 3, branched-chain-amino-acid aminotransferase, elongation 1- alpha 1, fatty acid synthase levels, cytosolic malate dehydrogenase and ATP synthase subunit alpha. These alterations point to increased proteolysis and lipogenesis rates and favoring of ATP generation. The restricted females showed down-regulated levels of L-lactate dehydrogenase perilipin-1, mitochondrial branched-chain alpha-keto acid dehydrogenase E1, and transketolase. These findings suggest impairment of glycemic control, stimulation of lipolysis and inhibition of proteolysis, pentose phosphate pathway and lipogenesis rates. In both genders, several proteins involved in oxidative stress and inflammation were affected, in a pattern compatible with impairment of these responses. CONCLUSIONS: The proteomic analysis of adipose tissue showed that, although IUGR affected pathways of substrate and energy metabolism in both males and females, important gender differences were evident. While IUGR males displayed alterations pointing to a predisposition to later development of obesity, the alterations observed in IUGR females pointed to a metabolic status of established obesity, in agreement with their increased fat pads mass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12953-015-0088-z) contains supplementary material, which is available to authorized users. BioMed Central 2015-12-02 /pmc/articles/PMC4667418/ /pubmed/26633942 http://dx.doi.org/10.1186/s12953-015-0088-z Text en © de Souza et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research de Souza, Adriana Pereira Pedroso, Amanda Paula Watanabe, Regina Lúcia Harumi Dornellas, Ana Paula Segantine Boldarine, Valter Tadeu Laure, Helen Julie do Nascimento, Claudia Maria Oller Oyama, Lila Missae Rosa, José Cesar Ribeiro, Eliane Beraldi Gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach |
title | Gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach |
title_full | Gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach |
title_fullStr | Gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach |
title_full_unstemmed | Gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach |
title_short | Gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach |
title_sort | gender-specific effects of intrauterine growth restriction on the adipose tissue of adult rats: a proteomic approach |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667418/ https://www.ncbi.nlm.nih.gov/pubmed/26633942 http://dx.doi.org/10.1186/s12953-015-0088-z |
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