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Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca(2+) and ERK1/ERK2 signaling

BACKGROUND: Pearl millet (PM), i.e., Pennisetum glaucum, is widely grown in Africa and known for its anti-oxidant and anti-hyperlipidemic properties. METHODS: The P. glaucum grains were obtained from the region of Ouled Aïssa (South of Algeria).  We assessed the effects of phenolic compounds and lip...

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Autores principales: Nani, Abdelhafid, Belarbi, Meriem, Ksouri-Megdiche, Wided, Abdoul-Azize, Souleymane, Benammar, Chahid, Ghiringhelli, François, Hichami, Aziz, Khan, Naim Akhtar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667445/
https://www.ncbi.nlm.nih.gov/pubmed/26627682
http://dx.doi.org/10.1186/s12906-015-0946-3
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author Nani, Abdelhafid
Belarbi, Meriem
Ksouri-Megdiche, Wided
Abdoul-Azize, Souleymane
Benammar, Chahid
Ghiringhelli, François
Hichami, Aziz
Khan, Naim Akhtar
author_facet Nani, Abdelhafid
Belarbi, Meriem
Ksouri-Megdiche, Wided
Abdoul-Azize, Souleymane
Benammar, Chahid
Ghiringhelli, François
Hichami, Aziz
Khan, Naim Akhtar
author_sort Nani, Abdelhafid
collection PubMed
description BACKGROUND: Pearl millet (PM), i.e., Pennisetum glaucum, is widely grown in Africa and known for its anti-oxidant and anti-hyperlipidemic properties. METHODS: The P. glaucum grains were obtained from the region of Ouled Aïssa (South of Algeria).  We assessed the effects of phenolic compounds and lipids, extracted from seeds of P. glaucum, on rat lymphocyte proliferation, activated by phorbol 12-myristate 13-acetate and ionomycin. In order to explore signaling pathway, triggered by these compounds, we assessed interleukin-2 (IL-2) mRNA expression and extracellular signal-regulated kinase-1/2 (ERK1/ERK2) phosphorylation. Finally, we determined increases in free intracellular Ca(2+) concentrations, [Ca(2+)]i, by employing Fura-2/AM in rat lymphocytes. RESULTS: The composition of P. glaucum grains in polyphenols was estimated to be 1660 µg gallic acid equivalents (GAE)/g. Lipids represented 4.5 %, and more than 72% of the fatty acids belonged to unsaturated family. Our investigation showed that both lipid and phenolic compounds inhibited mitogen-induced T-cell proliferation. Compared with phenolic compounds, lipids exerted weaker effects on ERK-1/ERK2 phosphorylation and Ca(2+) signaling in mitogen-activated T-cells. CONCLUSION: We conclude that the immunomodulatory effects of P. glaucum could be contributed by its phenolic and lipid contents.
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spelling pubmed-46674452015-12-03 Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca(2+) and ERK1/ERK2 signaling Nani, Abdelhafid Belarbi, Meriem Ksouri-Megdiche, Wided Abdoul-Azize, Souleymane Benammar, Chahid Ghiringhelli, François Hichami, Aziz Khan, Naim Akhtar BMC Complement Altern Med Research Article BACKGROUND: Pearl millet (PM), i.e., Pennisetum glaucum, is widely grown in Africa and known for its anti-oxidant and anti-hyperlipidemic properties. METHODS: The P. glaucum grains were obtained from the region of Ouled Aïssa (South of Algeria).  We assessed the effects of phenolic compounds and lipids, extracted from seeds of P. glaucum, on rat lymphocyte proliferation, activated by phorbol 12-myristate 13-acetate and ionomycin. In order to explore signaling pathway, triggered by these compounds, we assessed interleukin-2 (IL-2) mRNA expression and extracellular signal-regulated kinase-1/2 (ERK1/ERK2) phosphorylation. Finally, we determined increases in free intracellular Ca(2+) concentrations, [Ca(2+)]i, by employing Fura-2/AM in rat lymphocytes. RESULTS: The composition of P. glaucum grains in polyphenols was estimated to be 1660 µg gallic acid equivalents (GAE)/g. Lipids represented 4.5 %, and more than 72% of the fatty acids belonged to unsaturated family. Our investigation showed that both lipid and phenolic compounds inhibited mitogen-induced T-cell proliferation. Compared with phenolic compounds, lipids exerted weaker effects on ERK-1/ERK2 phosphorylation and Ca(2+) signaling in mitogen-activated T-cells. CONCLUSION: We conclude that the immunomodulatory effects of P. glaucum could be contributed by its phenolic and lipid contents. BioMed Central 2015-12-01 /pmc/articles/PMC4667445/ /pubmed/26627682 http://dx.doi.org/10.1186/s12906-015-0946-3 Text en © Nani et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Nani, Abdelhafid
Belarbi, Meriem
Ksouri-Megdiche, Wided
Abdoul-Azize, Souleymane
Benammar, Chahid
Ghiringhelli, François
Hichami, Aziz
Khan, Naim Akhtar
Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca(2+) and ERK1/ERK2 signaling
title Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca(2+) and ERK1/ERK2 signaling
title_full Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca(2+) and ERK1/ERK2 signaling
title_fullStr Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca(2+) and ERK1/ERK2 signaling
title_full_unstemmed Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca(2+) and ERK1/ERK2 signaling
title_short Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca(2+) and ERK1/ERK2 signaling
title_sort effects of polyphenols and lipids from pennisetum glaucum grains on t-cell activation: modulation of ca(2+) and erk1/erk2 signaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667445/
https://www.ncbi.nlm.nih.gov/pubmed/26627682
http://dx.doi.org/10.1186/s12906-015-0946-3
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