Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology

Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome sca...

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Detalles Bibliográficos
Autores principales: Gasser, Brigitte, Steiger, Matthias G., Mattanovich, Diethard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Commentary
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667464/
https://www.ncbi.nlm.nih.gov/pubmed/26627685
http://dx.doi.org/10.1186/s12934-015-0387-1
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author Gasser, Brigitte
Steiger, Matthias G.
Mattanovich, Diethard
author_facet Gasser, Brigitte
Steiger, Matthias G.
Mattanovich, Diethard
author_sort Gasser, Brigitte
collection PubMed
description Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome scale transcriptomics data for the primary selection of methanol inducible promoters of the yeast Pichia pastoris (Komagataella sp.). Such a promoter collection can serve as an excellent toolbox for cell and metabolic engineering, and for gene expression to produce heterologous proteins.
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spelling pubmed-46674642015-12-03 Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology Gasser, Brigitte Steiger, Matthias G. Mattanovich, Diethard Microb Cell Fact Commentary Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome scale transcriptomics data for the primary selection of methanol inducible promoters of the yeast Pichia pastoris (Komagataella sp.). Such a promoter collection can serve as an excellent toolbox for cell and metabolic engineering, and for gene expression to produce heterologous proteins. BioMed Central 2015-12-02 /pmc/articles/PMC4667464/ /pubmed/26627685 http://dx.doi.org/10.1186/s12934-015-0387-1 Text en © Gasser et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Commentary
Gasser, Brigitte
Steiger, Matthias G.
Mattanovich, Diethard
Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
title Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
title_full Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
title_fullStr Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
title_full_unstemmed Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
title_short Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
title_sort methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667464/
https://www.ncbi.nlm.nih.gov/pubmed/26627685
http://dx.doi.org/10.1186/s12934-015-0387-1
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