Cargando…

Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology

Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome sca...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gasser, Brigitte, Steiger, Matthias G., Mattanovich, Diethard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Commentary
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667464/ https://www.ncbi.nlm.nih.gov/pubmed/26627685 http://dx.doi.org/10.1186/s12934-015-0387-1

Ejemplares similares

Yeast biotechnology: teaching the old dog new tricks
por: Mattanovich, Diethard, et al.
Publicado: (2014)

Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications
por: Zavec, Domen, et al.
Publicado: (2020)

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris
por: Prielhofer, Roland, et al.
Publicado: (2013)

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris
por: Cámara, Elena, et al.
Publicado: (2017)

The bud tip is the cellular hot spot of protein secretion in yeasts
por: Puxbaum, Verena, et al.
Publicado: (2016)

Beyond alcohol oxidase: the methylotrophic yeast Komagataella phaffii utilizes methanol also with its native alcohol dehydrogenase Adh2
por: Zavec, Domen, et al.
Publicado: (2021)

GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris
por: Prielhofer, Roland, et al.
Publicado: (2017)

The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO(2)
por: Gassler, Thomas, et al.
Publicado: (2019)

Repressible promoters – A novel tool to generate conditional mutants in Pichia pastoris
por: Delic, Marizela, et al.
Publicado: (2013)

Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris
por: Nocon, Justyna, et al.
Publicado: (2016)

Creation of Stable Heterothallic Strains of Komagataella phaffii Enables Dissection of Mating Gene Regulation
por: Heistinger, Lina, et al.
Publicado: (2017)

In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response
por: Rebnegger, Corinna, et al.
Publicado: (2014)

Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris
por: Maurer, Michael, et al.
Publicado: (2006)

Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
por: Moser, Josef W., et al.
Publicado: (2017)

Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose‐regulated GTH1 promoter of Pichia pastoris
por: Prielhofer, Roland, et al.
Publicado: (2018)

Systematic sequence engineering enhances the induction strength of the glucose-regulated GTH1 promoter of Komagataella phaffii
por: Flores-Villegas, Mirelle, et al.
Publicado: (2023)

Open access to sequence: Browsing the Pichia pastoris genome
por: Mattanovich, Diethard, et al.
Publicado: (2009)

The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO(2) in the yeast Komagataella phaffii
por: Mitic, Bernd M., et al.
Publicado: (2023)

Biochemistry of microbial itaconic acid production
por: Steiger, Matthias G., et al.
Publicado: (2013)

Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions
por: Gasser, Brigitte, et al.
Publicado: (2007)

From rumen to industry
por: Sauer, Michael, et al.
Publicado: (2012)

Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast
por: Nielsen, Jens
Publicado: (2014)

Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii
por: Barbay, Diane, et al.
Publicado: (2021)

Non-genetic impact factors on chronological lifespan and stress resistance of baker’s yeast
por: Sauer, Michael, et al.
Publicado: (2016)

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris
por: Marsalek, Lukas, et al.
Publicado: (2019)

Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and RNA Scaffolds
por: Baumschabl, Michael, et al.
Publicado: (2020)

Freedom and Responsibility in Synthetic Genomics: The Synthetic Yeast Project
por: Sliva, Anna, et al.
Publicado: (2015)

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures
por: Burgard, Jonas, et al.
Publicado: (2017)

Synthetic two-species allodiploid and three-species allotetraploid Saccharomyces hybrids with euploid (complete) parental subgenomes
por: Antunovics, Zsuzsa, et al.
Publicado: (2023)

Functional inclusion bodies produced in the yeast Pichia pastoris
por: Rueda, Fabián, et al.
Publicado: (2016)

Recombinant protein production in the new Millennium
por: Villaverde, Antonio, et al.
Publicado: (2007)

mSphere of Influence: Synthetic Biology of Natural Product Biosynthesis
por: Walker, Mark C.
Publicado: (2020)

Identification and characterization of the Komagataella phaffii mating pheromone genes
por: Heistinger, Lina, et al.
Publicado: (2018)

Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level
por: Prielhofer, Roland, et al.
Publicado: (2015)

Commentary: Not many tools in the toolbox
por: Mahle, William T.
Publicado: (2021)

Systems-level organization of yeast methylotrophic lifestyle
por: Rußmayer, Hannes, et al.
Publicado: (2015)

Models for synthetic biology
por: Kaznessis, Yiannis N
Publicado: (2007)

Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation
por: Sauer, Michael, et al.
Publicado: (2004)

Downscaling screening cultures in a multifunctional bioreactor array‐on‐a‐chip for speeding up optimization of yeast‐based lactic acid bioproduction
por: Totaro, Damiano, et al.
Publicado: (2020)

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris
por: Mattanovich, Diethard, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_lnvna47012orcn0c8fddf44c78