Cargando…
Endostatin inhibits bradykinin-induced cardiac contraction
.Endogenous fragments of extracellular matrix are known to possess various biological effects. Levels of endostatin, a fragment of collagen type XVIII, increase in certain cardiac diseases, such as cardiac hypertrophy and myocardial infarction. However, the influence of endostatin on cardiac contrac...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667655/ https://www.ncbi.nlm.nih.gov/pubmed/26050753 http://dx.doi.org/10.1292/jvms.15-0200 |
_version_ | 1782403870004084736 |
---|---|
author | YASUDA, Jumpei TAKADA, Lila KAJIWARA, Yuka OKADA, Muneyosi YAMAWAKI, Hideyuki |
author_facet | YASUDA, Jumpei TAKADA, Lila KAJIWARA, Yuka OKADA, Muneyosi YAMAWAKI, Hideyuki |
author_sort | YASUDA, Jumpei |
collection | PubMed |
description | .Endogenous fragments of extracellular matrix are known to possess various biological effects. Levels of endostatin, a fragment of collagen type XVIII, increase in certain cardiac diseases, such as cardiac hypertrophy and myocardial infarction. However, the influence of endostatin on cardiac contraction has not been clarified. In the present study, we investigated the effects of endostatin on bradykinin-induced atrial contraction. Isometric contractile force of mouse isolated left atria induced by electrical current pulse was measured. Voltage-dependent calcium current of guinea pig ventricular myocytes was measured by a whole-cell patch-clamp technique. Endostatin (100–1,000 ng/ml) alone treatment had no influence on left atrial contraction. On the other hand, pretreatment with endostatin (300 ng/ml) significantly inhibited bradykinin (1 µM)-induced contraction and voltage-dependent calcium current. These data suggest that endostatin may decrease bradykinin-induced cardiac contraction perhaps through the inhibition of voltage-dependent calcium channel. |
format | Online Article Text |
id | pubmed-4667655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46676552015-12-03 Endostatin inhibits bradykinin-induced cardiac contraction YASUDA, Jumpei TAKADA, Lila KAJIWARA, Yuka OKADA, Muneyosi YAMAWAKI, Hideyuki J Vet Med Sci Pharmacology .Endogenous fragments of extracellular matrix are known to possess various biological effects. Levels of endostatin, a fragment of collagen type XVIII, increase in certain cardiac diseases, such as cardiac hypertrophy and myocardial infarction. However, the influence of endostatin on cardiac contraction has not been clarified. In the present study, we investigated the effects of endostatin on bradykinin-induced atrial contraction. Isometric contractile force of mouse isolated left atria induced by electrical current pulse was measured. Voltage-dependent calcium current of guinea pig ventricular myocytes was measured by a whole-cell patch-clamp technique. Endostatin (100–1,000 ng/ml) alone treatment had no influence on left atrial contraction. On the other hand, pretreatment with endostatin (300 ng/ml) significantly inhibited bradykinin (1 µM)-induced contraction and voltage-dependent calcium current. These data suggest that endostatin may decrease bradykinin-induced cardiac contraction perhaps through the inhibition of voltage-dependent calcium channel. The Japanese Society of Veterinary Science 2015-06-05 2015-11 /pmc/articles/PMC4667655/ /pubmed/26050753 http://dx.doi.org/10.1292/jvms.15-0200 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Pharmacology YASUDA, Jumpei TAKADA, Lila KAJIWARA, Yuka OKADA, Muneyosi YAMAWAKI, Hideyuki Endostatin inhibits bradykinin-induced cardiac contraction |
title | Endostatin inhibits bradykinin-induced cardiac contraction |
title_full | Endostatin inhibits bradykinin-induced cardiac contraction |
title_fullStr | Endostatin inhibits bradykinin-induced cardiac contraction |
title_full_unstemmed | Endostatin inhibits bradykinin-induced cardiac contraction |
title_short | Endostatin inhibits bradykinin-induced cardiac contraction |
title_sort | endostatin inhibits bradykinin-induced cardiac contraction |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667655/ https://www.ncbi.nlm.nih.gov/pubmed/26050753 http://dx.doi.org/10.1292/jvms.15-0200 |
work_keys_str_mv | AT yasudajumpei endostatininhibitsbradykinininducedcardiaccontraction AT takadalila endostatininhibitsbradykinininducedcardiaccontraction AT kajiwarayuka endostatininhibitsbradykinininducedcardiaccontraction AT okadamuneyosi endostatininhibitsbradykinininducedcardiaccontraction AT yamawakihideyuki endostatininhibitsbradykinininducedcardiaccontraction |