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Combination of α-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells

α-Tomatine is a glycoalkaloid found in tomatoes and curcumin is a major yellow pigment of turmeric. In the present study, the combined effect of these two compounds on prostate cancer cells was studied. Treatment of different prostate cancer cells with curcumin or α-tomatine alone resulted in growth...

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Autores principales: Huang, Huarong, Chen, Xuan, Li, Dongli, He, Yan, Li, Yu, Du, Zhiyun, Zhang, Kun, DiPaola, Robert, Goodin, Susan, Zheng, Xi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668011/
https://www.ncbi.nlm.nih.gov/pubmed/26630272
http://dx.doi.org/10.1371/journal.pone.0144293
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author Huang, Huarong
Chen, Xuan
Li, Dongli
He, Yan
Li, Yu
Du, Zhiyun
Zhang, Kun
DiPaola, Robert
Goodin, Susan
Zheng, Xi
author_facet Huang, Huarong
Chen, Xuan
Li, Dongli
He, Yan
Li, Yu
Du, Zhiyun
Zhang, Kun
DiPaola, Robert
Goodin, Susan
Zheng, Xi
author_sort Huang, Huarong
collection PubMed
description α-Tomatine is a glycoalkaloid found in tomatoes and curcumin is a major yellow pigment of turmeric. In the present study, the combined effect of these two compounds on prostate cancer cells was studied. Treatment of different prostate cancer cells with curcumin or α-tomatine alone resulted in growth inhibition and apoptosis in a concentration-dependent manner. Combinations of α-tomatine and curcumin synergistically inhibited the growth and induced apoptosis in prostate cancer PC-3 cells. Effects of the α-tomatine and curcumin combination were associated with synergistic inhibition of NF-κB activity and a potent decrease in the expression of its downstream gene Bcl-2 in the cells. Moreover, strong decreases in the levels of phospho-Akt and phosphor-ERK1/2 were found in PC-3 cells treated with α-tomatine and curcumin in combination. In animal experiment, SCID mice with PC-3 xenograft tumors were treated with α-tomatine and curcumin. Combination of α-tomatine and curcumin more potently inhibited the growth of PC-3 tumors than either agent alone. Results from the present study indicate that α-tomatine in combination with curcumin may be an effective strategy for inhibiting the growth of prostate cancer.
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spelling pubmed-46680112015-12-10 Combination of α-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells Huang, Huarong Chen, Xuan Li, Dongli He, Yan Li, Yu Du, Zhiyun Zhang, Kun DiPaola, Robert Goodin, Susan Zheng, Xi PLoS One Research Article α-Tomatine is a glycoalkaloid found in tomatoes and curcumin is a major yellow pigment of turmeric. In the present study, the combined effect of these two compounds on prostate cancer cells was studied. Treatment of different prostate cancer cells with curcumin or α-tomatine alone resulted in growth inhibition and apoptosis in a concentration-dependent manner. Combinations of α-tomatine and curcumin synergistically inhibited the growth and induced apoptosis in prostate cancer PC-3 cells. Effects of the α-tomatine and curcumin combination were associated with synergistic inhibition of NF-κB activity and a potent decrease in the expression of its downstream gene Bcl-2 in the cells. Moreover, strong decreases in the levels of phospho-Akt and phosphor-ERK1/2 were found in PC-3 cells treated with α-tomatine and curcumin in combination. In animal experiment, SCID mice with PC-3 xenograft tumors were treated with α-tomatine and curcumin. Combination of α-tomatine and curcumin more potently inhibited the growth of PC-3 tumors than either agent alone. Results from the present study indicate that α-tomatine in combination with curcumin may be an effective strategy for inhibiting the growth of prostate cancer. Public Library of Science 2015-12-02 /pmc/articles/PMC4668011/ /pubmed/26630272 http://dx.doi.org/10.1371/journal.pone.0144293 Text en © 2015 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Huang, Huarong
Chen, Xuan
Li, Dongli
He, Yan
Li, Yu
Du, Zhiyun
Zhang, Kun
DiPaola, Robert
Goodin, Susan
Zheng, Xi
Combination of α-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells
title Combination of α-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells
title_full Combination of α-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells
title_fullStr Combination of α-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells
title_full_unstemmed Combination of α-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells
title_short Combination of α-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells
title_sort combination of α-tomatine and curcumin inhibits growth and induces apoptosis in human prostate cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668011/
https://www.ncbi.nlm.nih.gov/pubmed/26630272
http://dx.doi.org/10.1371/journal.pone.0144293
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