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Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells

BACKGROUND: Dystroglycan has recently been characterised in blood tissue cells, as part of the dystrophin glycoprotein complex involved in the differentiation process of neutrophils. PURPOSE: In the present study we have investigated the role of dystroglycan in the human promyelocytic leukemic cell...

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Autores principales: Escárcega-Tame, Marco Antonio, Martínez-Vieyra, Ivette, Alonso-Rangel, Lea, Cisneros, Bulmaro, Winder, Steve J., Cerecedo, Doris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668107/
https://www.ncbi.nlm.nih.gov/pubmed/26630171
http://dx.doi.org/10.1371/journal.pone.0144078
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author Escárcega-Tame, Marco Antonio
Martínez-Vieyra, Ivette
Alonso-Rangel, Lea
Cisneros, Bulmaro
Winder, Steve J.
Cerecedo, Doris
author_facet Escárcega-Tame, Marco Antonio
Martínez-Vieyra, Ivette
Alonso-Rangel, Lea
Cisneros, Bulmaro
Winder, Steve J.
Cerecedo, Doris
author_sort Escárcega-Tame, Marco Antonio
collection PubMed
description BACKGROUND: Dystroglycan has recently been characterised in blood tissue cells, as part of the dystrophin glycoprotein complex involved in the differentiation process of neutrophils. PURPOSE: In the present study we have investigated the role of dystroglycan in the human promyelocytic leukemic cell line Kasumi-1 differentiated to macrophage-like cells. METHODS: We characterised the pattern expression and subcellular distribution of dystroglycans in non-differentiated and differentiated Kasumi-1 cells. RESULTS: Our results demonstrated by WB and flow cytometer assays that during the differentiation process to macrophages, dystroglycans were down-regulated; these results were confirmed with qRT-PCR assays. Additionally, depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated Kasumi-1 cells, including morphology, migration and phagocytic activities although secretion of IL-1β and expression of markers of differentiation are not altered. CONCLUSION: Our findings strongly implicate dystroglycan as a key membrane adhesion protein involved in actin-based structures during the differentiation process in Kasumi-1 cells.
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spelling pubmed-46681072015-12-10 Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells Escárcega-Tame, Marco Antonio Martínez-Vieyra, Ivette Alonso-Rangel, Lea Cisneros, Bulmaro Winder, Steve J. Cerecedo, Doris PLoS One Research Article BACKGROUND: Dystroglycan has recently been characterised in blood tissue cells, as part of the dystrophin glycoprotein complex involved in the differentiation process of neutrophils. PURPOSE: In the present study we have investigated the role of dystroglycan in the human promyelocytic leukemic cell line Kasumi-1 differentiated to macrophage-like cells. METHODS: We characterised the pattern expression and subcellular distribution of dystroglycans in non-differentiated and differentiated Kasumi-1 cells. RESULTS: Our results demonstrated by WB and flow cytometer assays that during the differentiation process to macrophages, dystroglycans were down-regulated; these results were confirmed with qRT-PCR assays. Additionally, depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated Kasumi-1 cells, including morphology, migration and phagocytic activities although secretion of IL-1β and expression of markers of differentiation are not altered. CONCLUSION: Our findings strongly implicate dystroglycan as a key membrane adhesion protein involved in actin-based structures during the differentiation process in Kasumi-1 cells. Public Library of Science 2015-12-02 /pmc/articles/PMC4668107/ /pubmed/26630171 http://dx.doi.org/10.1371/journal.pone.0144078 Text en © 2015 Escárcega-Tame et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Escárcega-Tame, Marco Antonio
Martínez-Vieyra, Ivette
Alonso-Rangel, Lea
Cisneros, Bulmaro
Winder, Steve J.
Cerecedo, Doris
Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells
title Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells
title_full Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells
title_fullStr Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells
title_full_unstemmed Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells
title_short Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells
title_sort dystroglycan depletion impairs actin-dependent functions of differentiated kasumi-1 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668107/
https://www.ncbi.nlm.nih.gov/pubmed/26630171
http://dx.doi.org/10.1371/journal.pone.0144078
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