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Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion
The aim of this study was to investigate the effect of a polyphenol-rich Açaí seed extract (ASE, 300 mg/kg-1d-1) on adiposity and hepatic steatosis in mice that were fed a high-fat (HF) diet and its underlying mechanisms based on hepatic lipid metabolism and oxidative stress. Four groups were studie...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668108/ https://www.ncbi.nlm.nih.gov/pubmed/26630290 http://dx.doi.org/10.1371/journal.pone.0143721 |
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author | de Oliveira, Paola Raquel B. da Costa, Cristiane A. de Bem, Graziele F. Cordeiro, Viviane S. C. Santos, Izabelle B. de Carvalho, Lenize C. R. M. da Conceição, Ellen Paula S. Lisboa, Patrícia Cristina Ognibene, Dayane T. Sousa, Pergentino José C. Martins, Gabriel R. da Silva, Antônio Jorge R. de Moura, Roberto S. Resende, Angela C. |
author_facet | de Oliveira, Paola Raquel B. da Costa, Cristiane A. de Bem, Graziele F. Cordeiro, Viviane S. C. Santos, Izabelle B. de Carvalho, Lenize C. R. M. da Conceição, Ellen Paula S. Lisboa, Patrícia Cristina Ognibene, Dayane T. Sousa, Pergentino José C. Martins, Gabriel R. da Silva, Antônio Jorge R. de Moura, Roberto S. Resende, Angela C. |
author_sort | de Oliveira, Paola Raquel B. |
collection | PubMed |
description | The aim of this study was to investigate the effect of a polyphenol-rich Açaí seed extract (ASE, 300 mg/kg-1d-1) on adiposity and hepatic steatosis in mice that were fed a high-fat (HF) diet and its underlying mechanisms based on hepatic lipid metabolism and oxidative stress. Four groups were studied: C57BL/6 mice that were fed with standard diet (10% fat, Control), 10% fat + ASE (ASE), 60% fat (HF), and 60% fat + ASE (HF + ASE) for 12 weeks. We evaluated the food intake, body weight gain, serum glucose and lipid profile, hepatic cholesterol and triacyglycerol (TG), hepatic expression of pAMPK, lipogenic proteins (SREBP-1c, pACC, ACC, HMG-CoA reductase) and cholesterol excretion transporters, ABCG5 and ABCG8. We also evaluated the steatosis in liver sections and oxidative stress. ASE reduced body weight gain, food intake, glucose levels, accumulation of cholesterol and TG in the liver, which was associated with a reduction of hepatic steatosis. The increased expressions of SREBP-1c and HMG-CoA reductase and reduced expressions of pAMPK and pACC/ACC in HF group were antagonized by ASE. The ABCG5 and ABCG8 transporters expressions were increased by the extract. The antioxidant effect of ASE was demonstrated in liver of HF mice by restoration of SOD, CAT and GPx activities and reduction of the increased levels of malondialdehyde and protein carbonylation. In conclusion, ASE substantially reduced the obesity and hepatic steatosis induced by HF diet by reducing lipogenesis, increasing cholesterol excretion and improving oxidative stress in the liver, providing a nutritional resource for prevention of obesity-related adiposity and hepatic steatosis. |
format | Online Article Text |
id | pubmed-4668108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46681082015-12-10 Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion de Oliveira, Paola Raquel B. da Costa, Cristiane A. de Bem, Graziele F. Cordeiro, Viviane S. C. Santos, Izabelle B. de Carvalho, Lenize C. R. M. da Conceição, Ellen Paula S. Lisboa, Patrícia Cristina Ognibene, Dayane T. Sousa, Pergentino José C. Martins, Gabriel R. da Silva, Antônio Jorge R. de Moura, Roberto S. Resende, Angela C. PLoS One Research Article The aim of this study was to investigate the effect of a polyphenol-rich Açaí seed extract (ASE, 300 mg/kg-1d-1) on adiposity and hepatic steatosis in mice that were fed a high-fat (HF) diet and its underlying mechanisms based on hepatic lipid metabolism and oxidative stress. Four groups were studied: C57BL/6 mice that were fed with standard diet (10% fat, Control), 10% fat + ASE (ASE), 60% fat (HF), and 60% fat + ASE (HF + ASE) for 12 weeks. We evaluated the food intake, body weight gain, serum glucose and lipid profile, hepatic cholesterol and triacyglycerol (TG), hepatic expression of pAMPK, lipogenic proteins (SREBP-1c, pACC, ACC, HMG-CoA reductase) and cholesterol excretion transporters, ABCG5 and ABCG8. We also evaluated the steatosis in liver sections and oxidative stress. ASE reduced body weight gain, food intake, glucose levels, accumulation of cholesterol and TG in the liver, which was associated with a reduction of hepatic steatosis. The increased expressions of SREBP-1c and HMG-CoA reductase and reduced expressions of pAMPK and pACC/ACC in HF group were antagonized by ASE. The ABCG5 and ABCG8 transporters expressions were increased by the extract. The antioxidant effect of ASE was demonstrated in liver of HF mice by restoration of SOD, CAT and GPx activities and reduction of the increased levels of malondialdehyde and protein carbonylation. In conclusion, ASE substantially reduced the obesity and hepatic steatosis induced by HF diet by reducing lipogenesis, increasing cholesterol excretion and improving oxidative stress in the liver, providing a nutritional resource for prevention of obesity-related adiposity and hepatic steatosis. Public Library of Science 2015-12-02 /pmc/articles/PMC4668108/ /pubmed/26630290 http://dx.doi.org/10.1371/journal.pone.0143721 Text en © 2015 de Oliveira et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article de Oliveira, Paola Raquel B. da Costa, Cristiane A. de Bem, Graziele F. Cordeiro, Viviane S. C. Santos, Izabelle B. de Carvalho, Lenize C. R. M. da Conceição, Ellen Paula S. Lisboa, Patrícia Cristina Ognibene, Dayane T. Sousa, Pergentino José C. Martins, Gabriel R. da Silva, Antônio Jorge R. de Moura, Roberto S. Resende, Angela C. Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion |
title |
Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion |
title_full |
Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion |
title_fullStr |
Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion |
title_full_unstemmed |
Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion |
title_short |
Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion |
title_sort | euterpe oleracea mart.-derived polyphenols protect mice from diet-induced obesity and fatty liver by regulating hepatic lipogenesis and cholesterol excretion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668108/ https://www.ncbi.nlm.nih.gov/pubmed/26630290 http://dx.doi.org/10.1371/journal.pone.0143721 |
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