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Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia

[Purpose] The purpose of this study was to investigate whether early hyperbaric oxygen is useful in rats with permanent cerebral ischemia, and whether its mechanism relates to the inhibition of the tumor necrosis factor-alpha-protein kinase C-alpha pathway. [Subjects] Healthy, male Sprague-Dawley ra...

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Autores principales: Yu, Min, Xue, Yixue, Liang, Weidi, Zhang, Yupeng, Zhang, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society of Physical Therapy Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668181/
https://www.ncbi.nlm.nih.gov/pubmed/26644690
http://dx.doi.org/10.1589/jpts.27.3271
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author Yu, Min
Xue, Yixue
Liang, Weidi
Zhang, Yupeng
Zhang, Zhiqiang
author_facet Yu, Min
Xue, Yixue
Liang, Weidi
Zhang, Yupeng
Zhang, Zhiqiang
author_sort Yu, Min
collection PubMed
description [Purpose] The purpose of this study was to investigate whether early hyperbaric oxygen is useful in rats with permanent cerebral ischemia, and whether its mechanism relates to the inhibition of the tumor necrosis factor-alpha-protein kinase C-alpha pathway. [Subjects] Healthy, male Sprague-Dawley rats (N = 108) were the subjects. [Methods] After middle cerebral artery occlusion models were successfully made, rats were randomly divided into sham-operated, cerebral ischemia, and hyperbaric oxygen groups. At 4 and 12 hours after modeling, the volume of cerebral infarction was determined by triphenyltetrazolium chloride staining, and brain water content was measured using the dry and wet method. The expression of tumor necrosis factor-alpha and protein kinase C-alpha in the ischemic penumbra tissue was measured using Western blot analysis. [Results] The data showed that at 4 and 12 hours after modeling, cerebral infarct volume and brain water content decreased in the hyperbaric oxygen group, and expression of tumor necrosis factor-alpha and phospho-protein kinase C-alpha in the ischemic penumbra tissue also decreased. [Conclusion] Our study demonstrates that early hyperbaric oxygen therapy has protective effects on brain tissue after cerebral ischemia, possibly via inhibition of tumor necrosis factor-alpha and phospho-protein kinase C-alpha.
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spelling pubmed-46681812015-12-07 Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia Yu, Min Xue, Yixue Liang, Weidi Zhang, Yupeng Zhang, Zhiqiang J Phys Ther Sci Original Article [Purpose] The purpose of this study was to investigate whether early hyperbaric oxygen is useful in rats with permanent cerebral ischemia, and whether its mechanism relates to the inhibition of the tumor necrosis factor-alpha-protein kinase C-alpha pathway. [Subjects] Healthy, male Sprague-Dawley rats (N = 108) were the subjects. [Methods] After middle cerebral artery occlusion models were successfully made, rats were randomly divided into sham-operated, cerebral ischemia, and hyperbaric oxygen groups. At 4 and 12 hours after modeling, the volume of cerebral infarction was determined by triphenyltetrazolium chloride staining, and brain water content was measured using the dry and wet method. The expression of tumor necrosis factor-alpha and protein kinase C-alpha in the ischemic penumbra tissue was measured using Western blot analysis. [Results] The data showed that at 4 and 12 hours after modeling, cerebral infarct volume and brain water content decreased in the hyperbaric oxygen group, and expression of tumor necrosis factor-alpha and phospho-protein kinase C-alpha in the ischemic penumbra tissue also decreased. [Conclusion] Our study demonstrates that early hyperbaric oxygen therapy has protective effects on brain tissue after cerebral ischemia, possibly via inhibition of tumor necrosis factor-alpha and phospho-protein kinase C-alpha. The Society of Physical Therapy Science 2015-10-30 2015-10 /pmc/articles/PMC4668181/ /pubmed/26644690 http://dx.doi.org/10.1589/jpts.27.3271 Text en 2015©by the Society of Physical Therapy Science. Published by IPEC Inc. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
Yu, Min
Xue, Yixue
Liang, Weidi
Zhang, Yupeng
Zhang, Zhiqiang
Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia
title Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia
title_full Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia
title_fullStr Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia
title_full_unstemmed Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia
title_short Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia
title_sort protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668181/
https://www.ncbi.nlm.nih.gov/pubmed/26644690
http://dx.doi.org/10.1589/jpts.27.3271
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