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Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia
[Purpose] The purpose of this study was to investigate whether early hyperbaric oxygen is useful in rats with permanent cerebral ischemia, and whether its mechanism relates to the inhibition of the tumor necrosis factor-alpha-protein kinase C-alpha pathway. [Subjects] Healthy, male Sprague-Dawley ra...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society of Physical Therapy Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668181/ https://www.ncbi.nlm.nih.gov/pubmed/26644690 http://dx.doi.org/10.1589/jpts.27.3271 |
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author | Yu, Min Xue, Yixue Liang, Weidi Zhang, Yupeng Zhang, Zhiqiang |
author_facet | Yu, Min Xue, Yixue Liang, Weidi Zhang, Yupeng Zhang, Zhiqiang |
author_sort | Yu, Min |
collection | PubMed |
description | [Purpose] The purpose of this study was to investigate whether early hyperbaric oxygen is useful in rats with permanent cerebral ischemia, and whether its mechanism relates to the inhibition of the tumor necrosis factor-alpha-protein kinase C-alpha pathway. [Subjects] Healthy, male Sprague-Dawley rats (N = 108) were the subjects. [Methods] After middle cerebral artery occlusion models were successfully made, rats were randomly divided into sham-operated, cerebral ischemia, and hyperbaric oxygen groups. At 4 and 12 hours after modeling, the volume of cerebral infarction was determined by triphenyltetrazolium chloride staining, and brain water content was measured using the dry and wet method. The expression of tumor necrosis factor-alpha and protein kinase C-alpha in the ischemic penumbra tissue was measured using Western blot analysis. [Results] The data showed that at 4 and 12 hours after modeling, cerebral infarct volume and brain water content decreased in the hyperbaric oxygen group, and expression of tumor necrosis factor-alpha and phospho-protein kinase C-alpha in the ischemic penumbra tissue also decreased. [Conclusion] Our study demonstrates that early hyperbaric oxygen therapy has protective effects on brain tissue after cerebral ischemia, possibly via inhibition of tumor necrosis factor-alpha and phospho-protein kinase C-alpha. |
format | Online Article Text |
id | pubmed-4668181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Society of Physical Therapy Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46681812015-12-07 Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia Yu, Min Xue, Yixue Liang, Weidi Zhang, Yupeng Zhang, Zhiqiang J Phys Ther Sci Original Article [Purpose] The purpose of this study was to investigate whether early hyperbaric oxygen is useful in rats with permanent cerebral ischemia, and whether its mechanism relates to the inhibition of the tumor necrosis factor-alpha-protein kinase C-alpha pathway. [Subjects] Healthy, male Sprague-Dawley rats (N = 108) were the subjects. [Methods] After middle cerebral artery occlusion models were successfully made, rats were randomly divided into sham-operated, cerebral ischemia, and hyperbaric oxygen groups. At 4 and 12 hours after modeling, the volume of cerebral infarction was determined by triphenyltetrazolium chloride staining, and brain water content was measured using the dry and wet method. The expression of tumor necrosis factor-alpha and protein kinase C-alpha in the ischemic penumbra tissue was measured using Western blot analysis. [Results] The data showed that at 4 and 12 hours after modeling, cerebral infarct volume and brain water content decreased in the hyperbaric oxygen group, and expression of tumor necrosis factor-alpha and phospho-protein kinase C-alpha in the ischemic penumbra tissue also decreased. [Conclusion] Our study demonstrates that early hyperbaric oxygen therapy has protective effects on brain tissue after cerebral ischemia, possibly via inhibition of tumor necrosis factor-alpha and phospho-protein kinase C-alpha. The Society of Physical Therapy Science 2015-10-30 2015-10 /pmc/articles/PMC4668181/ /pubmed/26644690 http://dx.doi.org/10.1589/jpts.27.3271 Text en 2015©by the Society of Physical Therapy Science. Published by IPEC Inc. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Article Yu, Min Xue, Yixue Liang, Weidi Zhang, Yupeng Zhang, Zhiqiang Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia |
title | Protection mechanism of early hyperbaric oxygen therapy in rats with
permanent cerebral ischemia |
title_full | Protection mechanism of early hyperbaric oxygen therapy in rats with
permanent cerebral ischemia |
title_fullStr | Protection mechanism of early hyperbaric oxygen therapy in rats with
permanent cerebral ischemia |
title_full_unstemmed | Protection mechanism of early hyperbaric oxygen therapy in rats with
permanent cerebral ischemia |
title_short | Protection mechanism of early hyperbaric oxygen therapy in rats with
permanent cerebral ischemia |
title_sort | protection mechanism of early hyperbaric oxygen therapy in rats with
permanent cerebral ischemia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668181/ https://www.ncbi.nlm.nih.gov/pubmed/26644690 http://dx.doi.org/10.1589/jpts.27.3271 |
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